Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment
Background: Human adipose-derived stem cells (hADSCs) are population of cells isolated from adipose tissue. hADSCs have ability to differentiate along multiple cell lineages and secret verity of growth factors, which affect the neighboring cells. Previous studies had assessed the effects of hADSCs a...
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| Format: | Article |
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Isfahan University of Medical Sciences
2017-02-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/7040 |
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| author | Leila Naseri Noushin Amirpour Hamid Bahramian Hossein Salehi |
| author_facet | Leila Naseri Noushin Amirpour Hamid Bahramian Hossein Salehi |
| author_sort | Leila Naseri |
| collection | DOAJ |
| description | Background: Human adipose-derived stem cells (hADSCs) are population of cells isolated from adipose tissue. hADSCs have ability to differentiate along multiple cell lineages and secret verity of growth factors, which affect the neighboring cells. Previous studies had assessed the effects of hADSCs and their conditioned medium on neural cells. To our knowledge, there were no studies done on the effects of hADSCs on retinal cells in vitro. Therefore, the aim of this study was to investigate the effect of hADSCs and their conditioned medium on retinal cell survival and migration in vitro.
Methods: The adipose tissue samples were obtained from a healthy donor after signing the consent. The stem cells were isolated and expanded through several subcultures. Rat retinal explants were isolated and cultured on coated wells. Retinal explants were co-cultured with hADSCs (direct) and their conditioned medium (indirect) for 1, 7 and 14 days.
Findings: There were significant differences (P < 0.05) in cell survival and migration between co-culture groups (Retina/hADSCs and Retina/Conditioned medium) and control group (Retina) at the day 14.
Conclusion: These data suggest that hADSCs and their conditioned medium improve retinal cell survival and migration in vitro culture environment. We hope that our study paves the way to use these cells for treatment of retinal degenerative diseases. |
| first_indexed | 2024-03-12T10:29:29Z |
| format | Article |
| id | doaj.art-24aa807faeb949d995d898596b253058 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T10:29:29Z |
| publishDate | 2017-02-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-24aa807faeb949d995d898596b2530582023-09-02T09:23:19ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2017-02-0134412154415492397Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro EnvironmentLeila Naseri0Noushin Amirpour1Hamid Bahramian2Hossein Salehi3MSc Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Human adipose-derived stem cells (hADSCs) are population of cells isolated from adipose tissue. hADSCs have ability to differentiate along multiple cell lineages and secret verity of growth factors, which affect the neighboring cells. Previous studies had assessed the effects of hADSCs and their conditioned medium on neural cells. To our knowledge, there were no studies done on the effects of hADSCs on retinal cells in vitro. Therefore, the aim of this study was to investigate the effect of hADSCs and their conditioned medium on retinal cell survival and migration in vitro. Methods: The adipose tissue samples were obtained from a healthy donor after signing the consent. The stem cells were isolated and expanded through several subcultures. Rat retinal explants were isolated and cultured on coated wells. Retinal explants were co-cultured with hADSCs (direct) and their conditioned medium (indirect) for 1, 7 and 14 days. Findings: There were significant differences (P < 0.05) in cell survival and migration between co-culture groups (Retina/hADSCs and Retina/Conditioned medium) and control group (Retina) at the day 14. Conclusion: These data suggest that hADSCs and their conditioned medium improve retinal cell survival and migration in vitro culture environment. We hope that our study paves the way to use these cells for treatment of retinal degenerative diseases.http://jims.mui.ac.ir/index.php/jims/article/view/7040Human adipose-derived stem cells (hADSCs)RetinaConditioned mediumCo-culture |
| spellingShingle | Leila Naseri Noushin Amirpour Hamid Bahramian Hossein Salehi Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment مجله دانشکده پزشکی اصفهان Human adipose-derived stem cells (hADSCs) Retina Conditioned medium Co-culture |
| title | Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment |
| title_full | Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment |
| title_fullStr | Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment |
| title_full_unstemmed | Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment |
| title_short | Investigating the Effect of Co-culturing Human Adipose-Derived Stem Cells on Retinal Cells Survival and Migration in Vitro Environment |
| title_sort | investigating the effect of co culturing human adipose derived stem cells on retinal cells survival and migration in vitro environment |
| topic | Human adipose-derived stem cells (hADSCs) Retina Conditioned medium Co-culture |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/7040 |
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