Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method
Abstract Background: Stress oxidative factors are known to causes diseases resulting from metabolic disorders. Therefore, preventing, or at least decreasing the amount of these factors may have a positive impact on prevention or improvement of the metabolic problems. Recently, the herbal medicines...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Arak Medical University
2017-03-01
|
| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-4742-en.pdf |
| _version_ | 1811282616401264640 |
|---|---|
| author | Masoud Soheili Mohammad Ali Khandan Mahmoud Salami |
| author_facet | Masoud Soheili Mohammad Ali Khandan Mahmoud Salami |
| author_sort | Masoud Soheili |
| collection | DOAJ |
| description | Abstract
Background: Stress oxidative factors are known to causes diseases resulting from metabolic disorders. Therefore, preventing, or at least decreasing the amount of these factors may have a positive impact on prevention or improvement of the metabolic problems. Recently, the herbal medicines are more considered due to more effectiveness. We designed the present study to evaluate anti-oxidant effect of aqueous extract of lavender (Lavandula angustifolia).
Materials and Methods: For extract preparation, the dry aerial part of lavender mixed with boiling water for 4 hours and then the container was filtered and condensed in a bain marie. Finally, the extract was powdered by freeze dryer. The anti-oxidant activities of the herbal medicine samples in 5, 10, 20 and 40 ppm concentrations were determined via DPPH method. This method is based on free radical scavenging of 2, 2- dipheny L-1-picrylhydrazyl(DPPH) reflected in the color and absorbance changes in spectrophotometery method in 520 nm. Finally the IC50 was calculated and compared with that of for vitamin C as a standard.
Results: All doses of the aqueous extract of the lavender showed dose- dependent potent anti-oxidant activity, So that, their differences were significant compared to control sample. The IC50 of the herbal medicine was 24.66 ppm that was less than the vitamin C of 2.3 ppm.
Conclusion: As a potent anti-oxidant, the lavender aqueous extract can be effective in treatment of metabolic diseases. |
| first_indexed | 2024-04-13T01:55:48Z |
| format | Article |
| id | doaj.art-24ae8fd04ad147c3863416672e20fd3a |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-04-13T01:55:48Z |
| publishDate | 2017-03-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-24ae8fd04ad147c3863416672e20fd3a2022-12-22T03:07:46ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2017-03-0119127077Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH MethodMasoud Soheili0Mohammad Ali Khandan1Mahmoud Salami2PhD in Applied Proteomics, Student Research Committee, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Dentistry Student, Kashan University of Medical Sciences, Kashan, IranProfessor, PhD of Physiology, Physiology Research Center, Kashan University of Medical Sciences, Kashan, IranAbstract Background: Stress oxidative factors are known to causes diseases resulting from metabolic disorders. Therefore, preventing, or at least decreasing the amount of these factors may have a positive impact on prevention or improvement of the metabolic problems. Recently, the herbal medicines are more considered due to more effectiveness. We designed the present study to evaluate anti-oxidant effect of aqueous extract of lavender (Lavandula angustifolia). Materials and Methods: For extract preparation, the dry aerial part of lavender mixed with boiling water for 4 hours and then the container was filtered and condensed in a bain marie. Finally, the extract was powdered by freeze dryer. The anti-oxidant activities of the herbal medicine samples in 5, 10, 20 and 40 ppm concentrations were determined via DPPH method. This method is based on free radical scavenging of 2, 2- dipheny L-1-picrylhydrazyl(DPPH) reflected in the color and absorbance changes in spectrophotometery method in 520 nm. Finally the IC50 was calculated and compared with that of for vitamin C as a standard. Results: All doses of the aqueous extract of the lavender showed dose- dependent potent anti-oxidant activity, So that, their differences were significant compared to control sample. The IC50 of the herbal medicine was 24.66 ppm that was less than the vitamin C of 2.3 ppm. Conclusion: As a potent anti-oxidant, the lavender aqueous extract can be effective in treatment of metabolic diseases.http://amuj.arakmu.ac.ir/article-1-4742-en.pdfAnti-oxidantAqueous extractLavandula angustifoliaMetabolic disorders |
| spellingShingle | Masoud Soheili Mohammad Ali Khandan Mahmoud Salami Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Anti-oxidant Aqueous extract Lavandula angustifolia Metabolic disorders |
| title | Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method |
| title_full | Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method |
| title_fullStr | Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method |
| title_full_unstemmed | Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method |
| title_short | Evaluation of Anti-Oxidant Activity of Lavandula angustifolia using DPPH Method |
| title_sort | evaluation of anti oxidant activity of lavandula angustifolia using dpph method |
| topic | Anti-oxidant Aqueous extract Lavandula angustifolia Metabolic disorders |
| url | http://amuj.arakmu.ac.ir/article-1-4742-en.pdf |
| work_keys_str_mv | AT masoudsoheili evaluationofantioxidantactivityoflavandulaangustifoliausingdpphmethod AT mohammadalikhandan evaluationofantioxidantactivityoflavandulaangustifoliausingdpphmethod AT mahmoudsalami evaluationofantioxidantactivityoflavandulaangustifoliausingdpphmethod |