Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load
Ingredients rich in phenolic compounds and antioxidants of winemaking wastes, which play an important role in the prevention of various diseases and the control of viruses, are being explored. Currently, there is a concern about honeybee population loss, with deformed wing virus (DWV) being the most...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/54 |
| _version_ | 1797446875008204800 |
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| author | Guillermo Pascual Diego Silva Marisol Vargas Mario Aranda Juan Antonio Cañumir María Dolores López |
| author_facet | Guillermo Pascual Diego Silva Marisol Vargas Mario Aranda Juan Antonio Cañumir María Dolores López |
| author_sort | Guillermo Pascual |
| collection | DOAJ |
| description | Ingredients rich in phenolic compounds and antioxidants of winemaking wastes, which play an important role in the prevention of various diseases and the control of viruses, are being explored. Currently, there is a concern about honeybee population loss, with deformed wing virus (DWV) being the most common virus infecting apiaries and one of the main causes of honeybee decline. Hence, the effect of grape pomace powder (GPP) as a dietary supplement to enhance the immune system of honeybees affected by DWV was evaluated. The characteristics of the ingredient GPP, obtained by spray-drying, revealed a high anthocyanin content (1102.45 mg 100 g<sup>−1</sup>), and it was applied at doses of 0.5, 1, 2.5 and 5% as a dietary supplement for bees infected by DWV. The results showed that the GPP treatments strengthened the immune response of honeybees against DWV. Moreover, the expression of the Relish gene was significantly higher in bees fed with GPP compared to the infected control. This study, which is framed in the search of food waste valorization for environmental sustainability, proves the feasibility of using grape wastes as dietary supplements for pollinators, and provides knowledge of the influence of polyphenols on the expression profiles of immune-related genes in honeybees. |
| first_indexed | 2024-03-09T13:46:52Z |
| format | Article |
| id | doaj.art-24b5e54b7caf48099741aa0f1507d6dc |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:46:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-24b5e54b7caf48099741aa0f1507d6dc2023-11-30T20:57:41ZengMDPI AGAntioxidants2076-39212022-12-011215410.3390/antiox12010054Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) LoadGuillermo Pascual0Diego Silva1Marisol Vargas2Mario Aranda3Juan Antonio Cañumir4María Dolores López5Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Vicente Méndez #595, Chillán 3780000, ChileDepartamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Vicente Méndez #595, Chillán 3780000, ChileDepartamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Vicente Méndez #595, Chillán 3780000, ChileLaboratorio de Investigación en Fármacos y Alimentos, Departamento de Farmacia, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Macul, Santiago 7810000, ChileLaboratorio de Bioprocesos, Departamento de Agroindustría, Facultad de Ingenería Agrícola, Universidad de Concepción, Vicente Méndez #595, Chillán 3780000, ChileDepartamento de Producción Vegetal, Facultad de Agronomía, Universidad de Concepción, Vicente Méndez #595, Chillán 3780000, ChileIngredients rich in phenolic compounds and antioxidants of winemaking wastes, which play an important role in the prevention of various diseases and the control of viruses, are being explored. Currently, there is a concern about honeybee population loss, with deformed wing virus (DWV) being the most common virus infecting apiaries and one of the main causes of honeybee decline. Hence, the effect of grape pomace powder (GPP) as a dietary supplement to enhance the immune system of honeybees affected by DWV was evaluated. The characteristics of the ingredient GPP, obtained by spray-drying, revealed a high anthocyanin content (1102.45 mg 100 g<sup>−1</sup>), and it was applied at doses of 0.5, 1, 2.5 and 5% as a dietary supplement for bees infected by DWV. The results showed that the GPP treatments strengthened the immune response of honeybees against DWV. Moreover, the expression of the Relish gene was significantly higher in bees fed with GPP compared to the infected control. This study, which is framed in the search of food waste valorization for environmental sustainability, proves the feasibility of using grape wastes as dietary supplements for pollinators, and provides knowledge of the influence of polyphenols on the expression profiles of immune-related genes in honeybees.https://www.mdpi.com/2076-3921/12/1/54winemaking industryanthocyanins<i>Apis mellifera</i>antiviral activityagro-sustainabilityfood waste valorization |
| spellingShingle | Guillermo Pascual Diego Silva Marisol Vargas Mario Aranda Juan Antonio Cañumir María Dolores López Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load Antioxidants winemaking industry anthocyanins <i>Apis mellifera</i> antiviral activity agro-sustainability food waste valorization |
| title | Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load |
| title_full | Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load |
| title_fullStr | Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load |
| title_full_unstemmed | Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load |
| title_short | Dietary Supplement of Grape Wastes Enhances Honeybee Immune System and Reduces Deformed Wing Virus (DWV) Load |
| title_sort | dietary supplement of grape wastes enhances honeybee immune system and reduces deformed wing virus dwv load |
| topic | winemaking industry anthocyanins <i>Apis mellifera</i> antiviral activity agro-sustainability food waste valorization |
| url | https://www.mdpi.com/2076-3921/12/1/54 |
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