SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer
Infiltration of diverse cell types into tumor microenvironment plays a critical role in cancer progression including metastasis. We previously reported that SFMBT2 (Scm-like with four mbt domains 2) regulates the expression of matrix metalloproteinases (MMPs) and migration and invasion of cancer cel...
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MDPI AG
2020-09-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/9/2718 |
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| author | Jungsug Gwak Hayan Jeong Kwanghyun Lee Jee Yoon Shin Taejun Sim Jungtae Na Jongchan Kim Bong-Gun Ju |
| author_facet | Jungsug Gwak Hayan Jeong Kwanghyun Lee Jee Yoon Shin Taejun Sim Jungtae Na Jongchan Kim Bong-Gun Ju |
| author_sort | Jungsug Gwak |
| collection | DOAJ |
| description | Infiltration of diverse cell types into tumor microenvironment plays a critical role in cancer progression including metastasis. We previously reported that SFMBT2 (Scm-like with four mbt domains 2) regulates the expression of matrix metalloproteinases (MMPs) and migration and invasion of cancer cells in prostate cancer. Here we investigated whether the down-regulation of SFMBT2 regulates the infiltration of preadipocytes and tumor-associated macrophages (TAMs) in prostate cancer. We found that the down-regulation of SFMBT2 promotes the infiltration of preadipocytes and TAMs through up-regulation of CXCL8, CCL2, CXCL10, and CCL20 expression in prostate cancer. Expression of CXCL8, CCL2, CXCL10, and CCL20 was also elevated in prostate cancer patients having a higher Gleason score (≥8), which had substantially lower SFMBT2 expression. We also found that the up-regulation of CXCL8, CCL2, CXCL10, and CCL20 expression is dependent on NF-κB activation in prostate cancer cells expressing a low level of SFMBT2. Moreover, increased IL-6 from infiltrated preadipocytes and TAMs promoted migration and invasion of prostate cancer cells expressing a low level of SFMBT2. Our study may suggest that SFMBT2 a critical regulator for the infiltration of preadipocytes and TAMs into the prostate tumor microenvironment. Thus, the regulation of SFMBT2 may provide a new therapeutic strategy to inhibit prostate cancer metastasis, and SFMBT2 could be used as a potential biomarker in prostate cancer metastasis. |
| first_indexed | 2024-03-10T16:08:27Z |
| format | Article |
| id | doaj.art-24b60136046b4a8c9236432e497b3b4e |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T16:08:27Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-24b60136046b4a8c9236432e497b3b4e2023-11-20T14:37:53ZengMDPI AGCancers2072-66942020-09-01129271810.3390/cancers12092718SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate CancerJungsug Gwak0Hayan Jeong1Kwanghyun Lee2Jee Yoon Shin3Taejun Sim4Jungtae Na5Jongchan Kim6Bong-Gun Ju7Department of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaDepartment of Life Science, Sogang University, Seoul 04107, KoreaInfiltration of diverse cell types into tumor microenvironment plays a critical role in cancer progression including metastasis. We previously reported that SFMBT2 (Scm-like with four mbt domains 2) regulates the expression of matrix metalloproteinases (MMPs) and migration and invasion of cancer cells in prostate cancer. Here we investigated whether the down-regulation of SFMBT2 regulates the infiltration of preadipocytes and tumor-associated macrophages (TAMs) in prostate cancer. We found that the down-regulation of SFMBT2 promotes the infiltration of preadipocytes and TAMs through up-regulation of CXCL8, CCL2, CXCL10, and CCL20 expression in prostate cancer. Expression of CXCL8, CCL2, CXCL10, and CCL20 was also elevated in prostate cancer patients having a higher Gleason score (≥8), which had substantially lower SFMBT2 expression. We also found that the up-regulation of CXCL8, CCL2, CXCL10, and CCL20 expression is dependent on NF-κB activation in prostate cancer cells expressing a low level of SFMBT2. Moreover, increased IL-6 from infiltrated preadipocytes and TAMs promoted migration and invasion of prostate cancer cells expressing a low level of SFMBT2. Our study may suggest that SFMBT2 a critical regulator for the infiltration of preadipocytes and TAMs into the prostate tumor microenvironment. Thus, the regulation of SFMBT2 may provide a new therapeutic strategy to inhibit prostate cancer metastasis, and SFMBT2 could be used as a potential biomarker in prostate cancer metastasis.https://www.mdpi.com/2072-6694/12/9/2718SFMBT2prostate cancer metastasispreadipocyteTAMsNF-κBchemokine |
| spellingShingle | Jungsug Gwak Hayan Jeong Kwanghyun Lee Jee Yoon Shin Taejun Sim Jungtae Na Jongchan Kim Bong-Gun Ju SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer Cancers SFMBT2 prostate cancer metastasis preadipocyte TAMs NF-κB chemokine |
| title | SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer |
| title_full | SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer |
| title_fullStr | SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer |
| title_full_unstemmed | SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer |
| title_short | SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer |
| title_sort | sfmbt2 mediated infiltration of preadipocytes and tams in prostate cancer |
| topic | SFMBT2 prostate cancer metastasis preadipocyte TAMs NF-κB chemokine |
| url | https://www.mdpi.com/2072-6694/12/9/2718 |
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