Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients
Abstract Background In the study on hand, we investigated the effect of IL-6 (−174 G/C; rs 1800795) and TGF-β1 (+915G/C; rs 1800471) gene polymorphisms on the susceptibility to Ovarian Cancer and their effect on plasma levels. IL-6 (−174 G/C) SNP was analyzed using mutagenically separated polymerase...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-03-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-022-00211-5 |
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| author | Zainab M. Almolakab Khalid A. El-Nesr Ehab Hassanin Mohamad Hassanin Rasha Elkaffas Ahmed Nabil |
| author_facet | Zainab M. Almolakab Khalid A. El-Nesr Ehab Hassanin Mohamad Hassanin Rasha Elkaffas Ahmed Nabil |
| author_sort | Zainab M. Almolakab |
| collection | DOAJ |
| description | Abstract Background In the study on hand, we investigated the effect of IL-6 (−174 G/C; rs 1800795) and TGF-β1 (+915G/C; rs 1800471) gene polymorphisms on the susceptibility to Ovarian Cancer and their effect on plasma levels. IL-6 (−174 G/C) SNP was analyzed using mutagenically separated polymerase chain reaction (MS-PCR) while TGF-β1 +915G/C (codon 25) SNP was investigated by the sequence-specific primer polymerase chain reaction (SSP-PCR). An enzyme-linked immunosorbent assay (ELISA) was used to quantify IL-6 and TGF-β1 plasma levels in 48 ovarian cancer patients and 48 normal controls. Results Regarding IL 6 (−174 G/C), a significant increase in CC and GC+CC genotypes parallel with the C allele was considered as risk factors for ovarian cancer; on the other hand, the G allele was considered as a protective factor for ovarian cancer. TGF-β1 (+915G/C) investigations showed a significant elevation in GC and GC+CC genotypes which can be considered as a risk factor for ovarian cancer. Plasma IL-6 and TGF-β1 were higher in ovarian cancer patients compared with controls. No specific genotype or allele could be responsible for the elevation of TGF-β1 in ovarian cancer patients’ plasma, while the highest significant value for IL6 in subjects carrying GG and CC genotypes in comparison with GC genotype. Conclusions This study supports an association of IL6 (−174G/C) and TGF-β1 (+915G/C) gene polymorphisms with the susceptibility to ovarian cancer. |
| first_indexed | 2024-12-19T23:42:09Z |
| format | Article |
| id | doaj.art-24be2dc97d6f4d2b97926efaedf011c8 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-19T23:42:09Z |
| publishDate | 2022-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-24be2dc97d6f4d2b97926efaedf011c82022-12-21T20:01:23ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432022-03-0111111910.1186/s43088-022-00211-5Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patientsZainab M. Almolakab0Khalid A. El-Nesr1Ehab Hassanin Mohamad Hassanin2Rasha Elkaffas3Ahmed Nabil4Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef UniversityPathology Department, Faculty of Veterinary Medicine, Beni-Suef UniversityObstetrics and Gynecology Department, Faculty of Medicine, Al-Azhar UniversityChemical Pathology Department, Faculty of Medicine, Cairo UniversityBiotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef UniversityAbstract Background In the study on hand, we investigated the effect of IL-6 (−174 G/C; rs 1800795) and TGF-β1 (+915G/C; rs 1800471) gene polymorphisms on the susceptibility to Ovarian Cancer and their effect on plasma levels. IL-6 (−174 G/C) SNP was analyzed using mutagenically separated polymerase chain reaction (MS-PCR) while TGF-β1 +915G/C (codon 25) SNP was investigated by the sequence-specific primer polymerase chain reaction (SSP-PCR). An enzyme-linked immunosorbent assay (ELISA) was used to quantify IL-6 and TGF-β1 plasma levels in 48 ovarian cancer patients and 48 normal controls. Results Regarding IL 6 (−174 G/C), a significant increase in CC and GC+CC genotypes parallel with the C allele was considered as risk factors for ovarian cancer; on the other hand, the G allele was considered as a protective factor for ovarian cancer. TGF-β1 (+915G/C) investigations showed a significant elevation in GC and GC+CC genotypes which can be considered as a risk factor for ovarian cancer. Plasma IL-6 and TGF-β1 were higher in ovarian cancer patients compared with controls. No specific genotype or allele could be responsible for the elevation of TGF-β1 in ovarian cancer patients’ plasma, while the highest significant value for IL6 in subjects carrying GG and CC genotypes in comparison with GC genotype. Conclusions This study supports an association of IL6 (−174G/C) and TGF-β1 (+915G/C) gene polymorphisms with the susceptibility to ovarian cancer.https://doi.org/10.1186/s43088-022-00211-5CytokineIL-6Ovarian cancerPolymorphismTGF-β1 |
| spellingShingle | Zainab M. Almolakab Khalid A. El-Nesr Ehab Hassanin Mohamad Hassanin Rasha Elkaffas Ahmed Nabil Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients Beni-Suef University Journal of Basic and Applied Sciences Cytokine IL-6 Ovarian cancer Polymorphism TGF-β1 |
| title | Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients |
| title_full | Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients |
| title_fullStr | Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients |
| title_full_unstemmed | Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients |
| title_short | Gene polymorphisms of Interleukin 6 (−174 G/C) and transforming growth factor β-1(+915 G/C) in ovarian cancer patients |
| title_sort | gene polymorphisms of interleukin 6 174 g c and transforming growth factor β 1 915 g c in ovarian cancer patients |
| topic | Cytokine IL-6 Ovarian cancer Polymorphism TGF-β1 |
| url | https://doi.org/10.1186/s43088-022-00211-5 |
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