Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs
High-grain diet is commonly used in intensive production to boost yield in short term, which may cause adverse effects such as rumen and colonic acidosis in ruminants. Maize is one of the key components of high-grain diet, and different processing methods of maize affect the digestive absorption and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1018284/full |
| _version_ | 1828091249596301312 |
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| author | Chao Cheng Chao Cheng Yuyang Yin Gaorui Bian |
| author_facet | Chao Cheng Chao Cheng Yuyang Yin Gaorui Bian |
| author_sort | Chao Cheng |
| collection | DOAJ |
| description | High-grain diet is commonly used in intensive production to boost yield in short term, which may cause adverse effects such as rumen and colonic acidosis in ruminants. Maize is one of the key components of high-grain diet, and different processing methods of maize affect the digestive absorption and gastrointestinal development of ruminants. To investigate the effects of maize form in high-grain diets on colonic fermentation and bacterial community of weaned lambs, twenty-two 2.5-month-old healthy Hu lambs were fed separately a maize meal low-grain diet (19.2% grain; CON), a maize meal high-grain diet (50.4% grain; CM), and a whole maize high-grain diet (50.4% grain; CG). After 7 weeks of feeding, the total volatile fatty acid concentration (P = 0.035) were significantly higher in lambs from CM than that from CON. The sequencing results of colonic content microbial composition revealed that the relative abundance of genera Parasutterella (P = 0.028), Comamonas (P = 0.031), Butyricicoccus (P = 0.049), and Olsenella (P = 0.010) were higher in CM than those in CON; compared with CM, the CG diet had the higher relative abundance of genera Bacteroides (P = 0.024) and Angelakisella (P = 0.020), while the lower relative abundance of genera Olsenella (P = 0.031) and Paraprevotella (P = 0.006). For colonic mucosal microbiota, the relative abundance of genera Duncaniella (P = 0.024), Succiniclasticum (P = 0.044), and Comamonas (P = 0.012) were significantly higher in CM than those in CON. In comparison, the relative abundance of genera Alistipes (P = 0.020) and Campylobacter (P = 0.017) were significantly lower. And the relative abundance of genera Colidextribacter (P = 0.005), Duncaniella (P = 0.032), Christensenella (P = 0.042), and Lawsonibacter (P = 0.018) were increased in the CG than those in the CM. Furthermore, the CG downregulated the relative abundance of genes encoding infectious-disease-parasitic (P = 0.049), cancer-specific-types (P = 0.049), and neurodegenerative-disease (P = 0.037) in colonic microbiota than those in the CM. Overall, these results indicated that maize with different grain sizes might influence the colonic health of weaned lambs by altering the composition of the colonic bacterial community. |
| first_indexed | 2024-04-11T06:09:24Z |
| format | Article |
| id | doaj.art-24be515b9f14479b819dc7f84838c277 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T06:09:24Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-24be515b9f14479b819dc7f84838c2772022-12-22T04:41:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10182841018284Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambsChao Cheng0Chao Cheng1Yuyang Yin2Gaorui Bian3College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaHuzhou Academy of Agricultural Sciences, Huzhou, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaHigh-grain diet is commonly used in intensive production to boost yield in short term, which may cause adverse effects such as rumen and colonic acidosis in ruminants. Maize is one of the key components of high-grain diet, and different processing methods of maize affect the digestive absorption and gastrointestinal development of ruminants. To investigate the effects of maize form in high-grain diets on colonic fermentation and bacterial community of weaned lambs, twenty-two 2.5-month-old healthy Hu lambs were fed separately a maize meal low-grain diet (19.2% grain; CON), a maize meal high-grain diet (50.4% grain; CM), and a whole maize high-grain diet (50.4% grain; CG). After 7 weeks of feeding, the total volatile fatty acid concentration (P = 0.035) were significantly higher in lambs from CM than that from CON. The sequencing results of colonic content microbial composition revealed that the relative abundance of genera Parasutterella (P = 0.028), Comamonas (P = 0.031), Butyricicoccus (P = 0.049), and Olsenella (P = 0.010) were higher in CM than those in CON; compared with CM, the CG diet had the higher relative abundance of genera Bacteroides (P = 0.024) and Angelakisella (P = 0.020), while the lower relative abundance of genera Olsenella (P = 0.031) and Paraprevotella (P = 0.006). For colonic mucosal microbiota, the relative abundance of genera Duncaniella (P = 0.024), Succiniclasticum (P = 0.044), and Comamonas (P = 0.012) were significantly higher in CM than those in CON. In comparison, the relative abundance of genera Alistipes (P = 0.020) and Campylobacter (P = 0.017) were significantly lower. And the relative abundance of genera Colidextribacter (P = 0.005), Duncaniella (P = 0.032), Christensenella (P = 0.042), and Lawsonibacter (P = 0.018) were increased in the CG than those in the CM. Furthermore, the CG downregulated the relative abundance of genes encoding infectious-disease-parasitic (P = 0.049), cancer-specific-types (P = 0.049), and neurodegenerative-disease (P = 0.037) in colonic microbiota than those in the CM. Overall, these results indicated that maize with different grain sizes might influence the colonic health of weaned lambs by altering the composition of the colonic bacterial community.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1018284/fulllambsmaizecolonic contentcolonic mucosabacterial community |
| spellingShingle | Chao Cheng Chao Cheng Yuyang Yin Gaorui Bian Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs Frontiers in Microbiology lambs maize colonic content colonic mucosa bacterial community |
| title | Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| title_full | Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| title_fullStr | Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| title_full_unstemmed | Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| title_short | Effects of whole maize high-grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| title_sort | effects of whole maize high grain diet feeding on colonic fermentation and bacterial community in weaned lambs |
| topic | lambs maize colonic content colonic mucosa bacterial community |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1018284/full |
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