Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial
The development of biocompatible nanocomposite hydrogels with effective wound healing/microbicidal properties is needed to bring out their distinguished characteristics in clinical applications. The positive interaction between graphene oxide/reduced graphene oxide (GO/rGO) and hydrogels and aloe ve...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/9/1/22 |
| _version_ | 1797442215072497664 |
|---|---|
| author | Dilip Kumar Shanmugam Yasasve Madhavan Aashabharathi Manimaran Gobi Saravanan Kaliaraj Karthik Ganesh Mohanraj Narthana Kandhasamy Kamalan Kirubaharan Amirtharaj Mosas |
| author_facet | Dilip Kumar Shanmugam Yasasve Madhavan Aashabharathi Manimaran Gobi Saravanan Kaliaraj Karthik Ganesh Mohanraj Narthana Kandhasamy Kamalan Kirubaharan Amirtharaj Mosas |
| author_sort | Dilip Kumar Shanmugam |
| collection | DOAJ |
| description | The development of biocompatible nanocomposite hydrogels with effective wound healing/microbicidal properties is needed to bring out their distinguished characteristics in clinical applications. The positive interaction between graphene oxide/reduced graphene oxide (GO/rGO) and hydrogels and aloe vera gel represents a strong strategy for the advancement of therapeutic approaches for wound healing. In this study, the synthesis, characterization, and angiogenic properties of graphene-based nanocomposite gels have been corroborated and substantiated through several in vitro and in vivo assays. In this respect, graphene oxide was synthesized by incorporating a modified Hummer’s method and ascertained by Raman spectroscopy. The obtained GO and rGO were uniformly dispersed into the aloe vera gel and hydrogel, respectively, as wound healing materials. These formulations were characterized via in vitro bio-chemical techniques and were found suitable for the appropriate cell viability, attachment, and proliferation. In addition, in vivo experiments were conducted using male Wistar rats. This revealed that the GO/rGO-based gels stimulated wound contraction and re-epithelialization compared to that of the non-treatment group. From the study, it is suggested that GO/rGO-based aloe vera gel can be recommended as a promising candidate for wound healing applications. |
| first_indexed | 2024-03-09T12:38:36Z |
| format | Article |
| id | doaj.art-24bea002d0224304accda288ec987b9f |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T12:38:36Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-24bea002d0224304accda288ec987b9f2023-11-30T22:21:19ZengMDPI AGGels2310-28612022-12-01912210.3390/gels9010022Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing BiomaterialDilip Kumar Shanmugam0Yasasve Madhavan1Aashabharathi Manimaran2Gobi Saravanan Kaliaraj3Karthik Ganesh Mohanraj4Narthana Kandhasamy5Kamalan Kirubaharan Amirtharaj Mosas6Centre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, IndiaDepartment of Oral Medicine and Radiology, Faculty of Dental Sciences, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, IndiaDepartment of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chembarambakkam, Chennai 600123, IndiaCentre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, IndiaDepartment of Anatomy, Saveetha Dental College and Hospital, Chennai 600077, IndiaCentre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, IndiaCoating Department, FunGlass—Centre for Functional and Surface Functionalized Glass, Alexander Dubcek University of Trencin, 91150 Trencin, SlovakiaThe development of biocompatible nanocomposite hydrogels with effective wound healing/microbicidal properties is needed to bring out their distinguished characteristics in clinical applications. The positive interaction between graphene oxide/reduced graphene oxide (GO/rGO) and hydrogels and aloe vera gel represents a strong strategy for the advancement of therapeutic approaches for wound healing. In this study, the synthesis, characterization, and angiogenic properties of graphene-based nanocomposite gels have been corroborated and substantiated through several in vitro and in vivo assays. In this respect, graphene oxide was synthesized by incorporating a modified Hummer’s method and ascertained by Raman spectroscopy. The obtained GO and rGO were uniformly dispersed into the aloe vera gel and hydrogel, respectively, as wound healing materials. These formulations were characterized via in vitro bio-chemical techniques and were found suitable for the appropriate cell viability, attachment, and proliferation. In addition, in vivo experiments were conducted using male Wistar rats. This revealed that the GO/rGO-based gels stimulated wound contraction and re-epithelialization compared to that of the non-treatment group. From the study, it is suggested that GO/rGO-based aloe vera gel can be recommended as a promising candidate for wound healing applications.https://www.mdpi.com/2310-2861/9/1/22microbial infectionaloe verahydrogelgraphene oxide (GO)reduced graphene oxide (rGO)wound healing |
| spellingShingle | Dilip Kumar Shanmugam Yasasve Madhavan Aashabharathi Manimaran Gobi Saravanan Kaliaraj Karthik Ganesh Mohanraj Narthana Kandhasamy Kamalan Kirubaharan Amirtharaj Mosas Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial Gels microbial infection aloe vera hydrogel graphene oxide (GO) reduced graphene oxide (rGO) wound healing |
| title | Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial |
| title_full | Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial |
| title_fullStr | Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial |
| title_full_unstemmed | Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial |
| title_short | Efficacy of Graphene-Based Nanocomposite Gels as a Promising Wound Healing Biomaterial |
| title_sort | efficacy of graphene based nanocomposite gels as a promising wound healing biomaterial |
| topic | microbial infection aloe vera hydrogel graphene oxide (GO) reduced graphene oxide (rGO) wound healing |
| url | https://www.mdpi.com/2310-2861/9/1/22 |
| work_keys_str_mv | AT dilipkumarshanmugam efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT yasasvemadhavan efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT aashabharathimanimaran efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT gobisaravanankaliaraj efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT karthikganeshmohanraj efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT narthanakandhasamy efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial AT kamalankirubaharanamirtharajmosas efficacyofgraphenebasednanocompositegelsasapromisingwoundhealingbiomaterial |