Diversity of root nodule bacteria from leguminous crops
In the present study, a total of 353 nodule-associated bacteria were isolated from 220 legume plant samples belonging to Cicer arietinum (85), Glycine max (74), Vigna radiata (21) and Cajanus cajan (40). A total of 224 bacteria were identified as fast-growing Rhizobium spp. on the basis of...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500142A.pdf |
| _version_ | 1811192575923585024 |
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| author | Agrawal Pooja Shukla Shruti |
| author_facet | Agrawal Pooja Shukla Shruti |
| author_sort | Agrawal Pooja |
| collection | DOAJ |
| description | In the present study, a total of 353 nodule-associated bacteria were isolated
from 220 legume plant samples belonging to Cicer arietinum (85), Glycine max
(74), Vigna radiata (21) and Cajanus cajan (40). A total of 224 bacteria were
identified as fast-growing Rhizobium spp. on the basis of differential
staining (Gram staining and carbol fuchsin staining) and biochemical tests.
All the isolates were tested for indole acetic acid production (IAA),
phosphate solubilization and siderophore production on plate assay. To
examine the effect of volatile organic metabolites (VOM) and water soluble
soil components (WSSC) on nodule bacteria, culture conditions were optimized
by observing the effects of various parameters such as pH, salt content and
temperatures on the growth of bacteria. Selected rhizobia were subjected to
random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA
restriction analysis (ARDRA) analysis to identify their species. On the basis
of RAPD and ARDRA, 10 isolates were identified as Rhizobium meliloti. In this
study, Rhizobium GO4, G16, G20, G77, S43, S81, M07, M37, A15 and A55 were
observed as the best candidates among the tested bacteria and can be further
used as potent bioinoculants. |
| first_indexed | 2024-04-11T23:54:55Z |
| format | Article |
| id | doaj.art-24c416a18a6542d8a07b44e89106236a |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-11T23:54:55Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-24c416a18a6542d8a07b44e89106236a2022-12-22T03:56:23ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168119520510.2298/ABS150504142A0354-46641500142ADiversity of root nodule bacteria from leguminous cropsAgrawal Pooja0Shukla Shruti1Dr.H.S. Gour Vishwavidyalaya, Department of Microbiology and Biotechnology, Sagar, IndiaYeungnam University, School of Biotechnology, Department of Food Science and Technology, Gyeongsan, Gyeongbuk, Republic of KoreaIn the present study, a total of 353 nodule-associated bacteria were isolated from 220 legume plant samples belonging to Cicer arietinum (85), Glycine max (74), Vigna radiata (21) and Cajanus cajan (40). A total of 224 bacteria were identified as fast-growing Rhizobium spp. on the basis of differential staining (Gram staining and carbol fuchsin staining) and biochemical tests. All the isolates were tested for indole acetic acid production (IAA), phosphate solubilization and siderophore production on plate assay. To examine the effect of volatile organic metabolites (VOM) and water soluble soil components (WSSC) on nodule bacteria, culture conditions were optimized by observing the effects of various parameters such as pH, salt content and temperatures on the growth of bacteria. Selected rhizobia were subjected to random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA) analysis to identify their species. On the basis of RAPD and ARDRA, 10 isolates were identified as Rhizobium meliloti. In this study, Rhizobium GO4, G16, G20, G77, S43, S81, M07, M37, A15 and A55 were observed as the best candidates among the tested bacteria and can be further used as potent bioinoculants.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500142A.pdflegumesrhizobiumRAPDARDRArhizobacteria |
| spellingShingle | Agrawal Pooja Shukla Shruti Diversity of root nodule bacteria from leguminous crops Archives of Biological Sciences legumes rhizobium RAPD ARDRA rhizobacteria |
| title | Diversity of root nodule bacteria from leguminous crops |
| title_full | Diversity of root nodule bacteria from leguminous crops |
| title_fullStr | Diversity of root nodule bacteria from leguminous crops |
| title_full_unstemmed | Diversity of root nodule bacteria from leguminous crops |
| title_short | Diversity of root nodule bacteria from leguminous crops |
| title_sort | diversity of root nodule bacteria from leguminous crops |
| topic | legumes rhizobium RAPD ARDRA rhizobacteria |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500142A.pdf |
| work_keys_str_mv | AT agrawalpooja diversityofrootnodulebacteriafromleguminouscrops AT shuklashruti diversityofrootnodulebacteriafromleguminouscrops |