Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i>
<i>Cheilinus undulatus</i> is a valuable seawater economic fish with tender meat, fresh taste, and high nutritional value; however, its population is rapidly declining because of massive fishing and habitat destruction. Assessing changes in genetic diversity and inbreeding levels is a ve...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/12/2000 |
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| author | Fangcao Zhao Liang Guo Nan Zhang Kecheng Zhu Jingwen Yang Baosuo Liu Huayang Guo Dianchang Zhang |
| author_facet | Fangcao Zhao Liang Guo Nan Zhang Kecheng Zhu Jingwen Yang Baosuo Liu Huayang Guo Dianchang Zhang |
| author_sort | Fangcao Zhao |
| collection | DOAJ |
| description | <i>Cheilinus undulatus</i> is a valuable seawater economic fish with tender meat, fresh taste, and high nutritional value; however, its population is rapidly declining because of massive fishing and habitat destruction. Assessing changes in genetic diversity and inbreeding levels is a very valuable monitoring tool, and multiplex PCR has the advantages of being time-efficient and economical. Here, we selected 12 pairs of polymorphic microsatellite loci, developed two multiplex PCR amplification systems based on these microsatellites, and used them to examine 30 <i>C. undulatus</i> specimens. The number of alleles (Na) for the 12 microsatellite markers ranged from 2 to 8. The effective allele number (Ne) ranged from 1.724 to 4.592. The expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.420 to 0.782 and 0.100 to 0.900, respectively. The polymorphic information content (PIC) ranged from 0.422 to 0.746, with a mean value of 0.557. 5 loci deviated from Hardy-Weinberg equilibrium (HWE, <i>p</i> < 0.05 after Bonferroni correction). The multiplex PCR amplification system established in this study provides a basis for germplasm identification, genetic diversity analysis, and assessment of the effects of accretion and release of <i>C. undulatus</i>. |
| first_indexed | 2024-03-09T16:13:37Z |
| format | Article |
| id | doaj.art-24c7b26b5f4a49e1ba409acf7d30f6b3 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-09T16:13:37Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-24c7b26b5f4a49e1ba409acf7d30f6b32023-11-24T15:57:56ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-12-011012200010.3390/jmse10122000Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i>Fangcao Zhao0Liang Guo1Nan Zhang2Kecheng Zhu3Jingwen Yang4Baosuo Liu5Huayang Guo6Dianchang Zhang7Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China<i>Cheilinus undulatus</i> is a valuable seawater economic fish with tender meat, fresh taste, and high nutritional value; however, its population is rapidly declining because of massive fishing and habitat destruction. Assessing changes in genetic diversity and inbreeding levels is a very valuable monitoring tool, and multiplex PCR has the advantages of being time-efficient and economical. Here, we selected 12 pairs of polymorphic microsatellite loci, developed two multiplex PCR amplification systems based on these microsatellites, and used them to examine 30 <i>C. undulatus</i> specimens. The number of alleles (Na) for the 12 microsatellite markers ranged from 2 to 8. The effective allele number (Ne) ranged from 1.724 to 4.592. The expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.420 to 0.782 and 0.100 to 0.900, respectively. The polymorphic information content (PIC) ranged from 0.422 to 0.746, with a mean value of 0.557. 5 loci deviated from Hardy-Weinberg equilibrium (HWE, <i>p</i> < 0.05 after Bonferroni correction). The multiplex PCR amplification system established in this study provides a basis for germplasm identification, genetic diversity analysis, and assessment of the effects of accretion and release of <i>C. undulatus</i>.https://www.mdpi.com/2077-1312/10/12/2000<i>Cheilinus undulatus</i>microsatellitemultiplex PCRgenetic diversity |
| spellingShingle | Fangcao Zhao Liang Guo Nan Zhang Kecheng Zhu Jingwen Yang Baosuo Liu Huayang Guo Dianchang Zhang Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> Journal of Marine Science and Engineering <i>Cheilinus undulatus</i> microsatellite multiplex PCR genetic diversity |
| title | Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> |
| title_full | Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> |
| title_fullStr | Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> |
| title_full_unstemmed | Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> |
| title_short | Establishment and Application of Microsatellite Multiplex PCR System for <i>Cheilinus undulatus</i> |
| title_sort | establishment and application of microsatellite multiplex pcr system for i cheilinus undulatus i |
| topic | <i>Cheilinus undulatus</i> microsatellite multiplex PCR genetic diversity |
| url | https://www.mdpi.com/2077-1312/10/12/2000 |
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