Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity
<i>Pleurotus eryngii</i> (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of <i>P. eryngii</i> is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elemen...
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MDPI AG
2020-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/4/889 |
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| author | Piotr Zięba Katarzyna Kała Anna Włodarczyk Agnieszka Szewczyk Edward Kunicki Agnieszka Sękara Bożena Muszyńska |
| author_facet | Piotr Zięba Katarzyna Kała Anna Włodarczyk Agnieszka Szewczyk Edward Kunicki Agnieszka Sękara Bożena Muszyńska |
| author_sort | Piotr Zięba |
| collection | DOAJ |
| description | <i>Pleurotus eryngii</i> (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of <i>P. eryngii</i> is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elements with a functional activity in the human body. In this study, the ability of <i>P. eryngii</i> mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of <i>P. eryngii</i> was determined as well. To enhance Se and Zn content in <i>P. eryngii</i> fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L<sup>−1</sup>, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L<sup>−1</sup>, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20 mg L<sup>−1</sup>. The content of organic compounds include phenolic compounds and lovastatin, which were determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of <i>P. eryngii</i> to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be different depending on the type of salt used. The present study also showed that conducting mycelium of <i>P. eryngii</i> in in vitro culture, with a higher content of zinc ions, can result in obtaining the materials with better antioxidant ability. The results of this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements. |
| first_indexed | 2024-12-12T20:04:37Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T20:04:37Z |
| publishDate | 2020-02-01 |
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| spelling | doaj.art-24c907c867684eaea3d4ba89209051c72022-12-22T00:13:40ZengMDPI AGMolecules1420-30492020-02-0125488910.3390/molecules25040889molecules25040889Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological ActivityPiotr Zięba0Katarzyna Kała1Anna Włodarczyk2Agnieszka Szewczyk3Edward Kunicki4Agnieszka Sękara5Bożena Muszyńska6Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, PolandDepartment of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, PolandDepartment of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31-425 Krakow, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland<i>Pleurotus eryngii</i> (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of <i>P. eryngii</i> is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elements with a functional activity in the human body. In this study, the ability of <i>P. eryngii</i> mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of <i>P. eryngii</i> was determined as well. To enhance Se and Zn content in <i>P. eryngii</i> fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L<sup>−1</sup>, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L<sup>−1</sup>, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20 mg L<sup>−1</sup>. The content of organic compounds include phenolic compounds and lovastatin, which were determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of <i>P. eryngii</i> to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be different depending on the type of salt used. The present study also showed that conducting mycelium of <i>P. eryngii</i> in in vitro culture, with a higher content of zinc ions, can result in obtaining the materials with better antioxidant ability. The results of this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements.https://www.mdpi.com/1420-3049/25/4/889biofortificationlovastatinphenoloic compounds<i>pleurotus eryngii</i>seleniumzinc |
| spellingShingle | Piotr Zięba Katarzyna Kała Anna Włodarczyk Agnieszka Szewczyk Edward Kunicki Agnieszka Sękara Bożena Muszyńska Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity Molecules biofortification lovastatin phenoloic compounds <i>pleurotus eryngii</i> selenium zinc |
| title | Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity |
| title_full | Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity |
| title_fullStr | Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity |
| title_full_unstemmed | Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity |
| title_short | Selenium and Zinc Biofortification of <i>Pleurotus eryngii</i> Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity |
| title_sort | selenium and zinc biofortification of i pleurotus eryngii i mycelium and fruiting bodies as a tool for controlling their biological activity |
| topic | biofortification lovastatin phenoloic compounds <i>pleurotus eryngii</i> selenium zinc |
| url | https://www.mdpi.com/1420-3049/25/4/889 |
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