Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process
The treatment of catechol via biocatalysis and adsorption with a commercial laccase immobilized on polyacrylonitrile/montmorillonite/graphene oxide (PAN/MMT/GO) composite nanofibers was evaluated with a homemade nanofibrous membrane reactor. The properties in this process of the immobilized laccase...
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MDPI AG
2014-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/3/3376 |
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| author | Qingqing Wang Jing Cui Guohui Li Jinning Zhang Dawei Li Fenglin Huang Qufu Wei |
| author_facet | Qingqing Wang Jing Cui Guohui Li Jinning Zhang Dawei Li Fenglin Huang Qufu Wei |
| author_sort | Qingqing Wang |
| collection | DOAJ |
| description | The treatment of catechol via biocatalysis and adsorption with a commercial laccase immobilized on polyacrylonitrile/montmorillonite/graphene oxide (PAN/MMT/GO) composite nanofibers was evaluated with a homemade nanofibrous membrane reactor. The properties in this process of the immobilized laccase on PAN, PAN/MMT as well as PAN/MMT/GO with different weight ratios of MMT and GO were investigated. These membranes were successfully applied for removal of catechol from an aqueous solution. Scanning electron microscope images revealed different morphologies of the enzyme aggregates on different supports. After incorporation of MMT or MMT/GO, the optimum pH showed an alkaline shift to 4, compared to 3.5 for laccase immobilized on pure PAN nanofibers. The optimum temperature was at 55 °C for all the immobilized enzymes. Besides, the addition of GO improved the operational stability and storage stability. A 39% ± 2.23% chemical oxygen demand (COD) removal from the catechol aqueous solution was achieved. Experimental results suggested that laccase, PAN, adsorbent nanoparticles (MMT/GO) can be combined together for catechol treatment in industrial applications. |
| first_indexed | 2024-12-24T00:44:05Z |
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| id | doaj.art-24cb9e019b864e0ab3c007a6c4023b31 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-24T00:44:05Z |
| publishDate | 2014-03-01 |
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| series | Molecules |
| spelling | doaj.art-24cb9e019b864e0ab3c007a6c4023b312022-12-21T17:23:51ZengMDPI AGMolecules1420-30492014-03-011933376338810.3390/molecules19033376molecules19033376Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption ProcessQingqing Wang0Jing Cui1Guohui Li2Jinning Zhang3Dawei Li4Fenglin Huang5Qufu Wei6Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, ChinaThe treatment of catechol via biocatalysis and adsorption with a commercial laccase immobilized on polyacrylonitrile/montmorillonite/graphene oxide (PAN/MMT/GO) composite nanofibers was evaluated with a homemade nanofibrous membrane reactor. The properties in this process of the immobilized laccase on PAN, PAN/MMT as well as PAN/MMT/GO with different weight ratios of MMT and GO were investigated. These membranes were successfully applied for removal of catechol from an aqueous solution. Scanning electron microscope images revealed different morphologies of the enzyme aggregates on different supports. After incorporation of MMT or MMT/GO, the optimum pH showed an alkaline shift to 4, compared to 3.5 for laccase immobilized on pure PAN nanofibers. The optimum temperature was at 55 °C for all the immobilized enzymes. Besides, the addition of GO improved the operational stability and storage stability. A 39% ± 2.23% chemical oxygen demand (COD) removal from the catechol aqueous solution was achieved. Experimental results suggested that laccase, PAN, adsorbent nanoparticles (MMT/GO) can be combined together for catechol treatment in industrial applications.http://www.mdpi.com/1420-3049/19/3/3376laccaseenzyme immobilizationadsorbentcatecholadsorption |
| spellingShingle | Qingqing Wang Jing Cui Guohui Li Jinning Zhang Dawei Li Fenglin Huang Qufu Wei Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process Molecules laccase enzyme immobilization adsorbent catechol adsorption |
| title | Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process |
| title_full | Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process |
| title_fullStr | Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process |
| title_full_unstemmed | Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process |
| title_short | Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process |
| title_sort | laccase immobilized on a pan adsorbents composite nanofibrous membrane for catechol treatment by a biocatalysis adsorption process |
| topic | laccase enzyme immobilization adsorbent catechol adsorption |
| url | http://www.mdpi.com/1420-3049/19/3/3376 |
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