Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202
Scenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope up the stress...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.585632/full |
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| author | Sujitha Balakrishnan Sulochana Sujitha Balakrishnan Sulochana Muthu Arumugam Muthu Arumugam |
| author_facet | Sujitha Balakrishnan Sulochana Sujitha Balakrishnan Sulochana Muthu Arumugam Muthu Arumugam |
| author_sort | Sujitha Balakrishnan Sulochana |
| collection | DOAJ |
| description | Scenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope up the stress condition. The nitrogen stress-induced biochemical changes in mitochondrion exhibits due to the oxidative stress-induced Reactive Oxygen species (ROS) generation at high membrane potential (Δψm). The predominant ROS generated during nitrogen starvation was H2O2, OH–, O2⋅− and to suppress them, scavenging enzymes such as peroxidase and catalase increased to about 23.16 and 0.79 U/ml as compared to control (20.2, 0.19 U/ml). The targeted metabolic analysis showed, stress-related non-proteinogenic amino acids and energy equivalents elevated during the initial hours of nitrogen starvation. The nitrogen stress-triggered biochemical and metabolic changes along with other cellular events eventually lead to lipid accumulation in S. quadricauda. |
| first_indexed | 2024-12-12T13:29:19Z |
| format | Article |
| id | doaj.art-24ce5ca1f07f4bebb4b7e5388716136a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T13:29:19Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-24ce5ca1f07f4bebb4b7e5388716136a2022-12-22T00:23:06ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.585632585632Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202Sujitha Balakrishnan Sulochana0Sujitha Balakrishnan Sulochana1Muthu Arumugam2Muthu Arumugam3Microbial Processes and Technology Division, Council of Scientific and Industrial Research – National Institute for Interdisciplinary Science and Technology, Trivandrum, IndiaAcademy of Scientific and Innovative Research, Ghaziabad, IndiaMicrobial Processes and Technology Division, Council of Scientific and Industrial Research – National Institute for Interdisciplinary Science and Technology, Trivandrum, IndiaAcademy of Scientific and Innovative Research, Ghaziabad, IndiaScenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope up the stress condition. The nitrogen stress-induced biochemical changes in mitochondrion exhibits due to the oxidative stress-induced Reactive Oxygen species (ROS) generation at high membrane potential (Δψm). The predominant ROS generated during nitrogen starvation was H2O2, OH–, O2⋅− and to suppress them, scavenging enzymes such as peroxidase and catalase increased to about 23.16 and 0.79 U/ml as compared to control (20.2, 0.19 U/ml). The targeted metabolic analysis showed, stress-related non-proteinogenic amino acids and energy equivalents elevated during the initial hours of nitrogen starvation. The nitrogen stress-triggered biochemical and metabolic changes along with other cellular events eventually lead to lipid accumulation in S. quadricauda.https://www.frontiersin.org/articles/10.3389/fbioe.2020.585632/fullmicroalgaebiofuelnitrogen stressReactive Oxygen speciesmitochondriametabolic changes |
| spellingShingle | Sujitha Balakrishnan Sulochana Sujitha Balakrishnan Sulochana Muthu Arumugam Muthu Arumugam Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 Frontiers in Bioengineering and Biotechnology microalgae biofuel nitrogen stress Reactive Oxygen species mitochondria metabolic changes |
| title | Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 |
| title_full | Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 |
| title_fullStr | Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 |
| title_full_unstemmed | Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 |
| title_short | Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation in Scenedesmus quadricauda CASA CC202 |
| title_sort | targeted metabolomic and biochemical changes during nitrogen stress mediated lipid accumulation in scenedesmus quadricauda casa cc202 |
| topic | microalgae biofuel nitrogen stress Reactive Oxygen species mitochondria metabolic changes |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2020.585632/full |
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