Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols
Lipopolysaccharide (LPS) induces host inflammatory responses and tissue injury and has been implicated in the pathogenesis of various age-related diseases such as acute respiratory distress syndrome, vascular diseases, and periodontal disease. Antioxidants, particularly vitamin E, have been shown to...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Redox Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231712000122 |
| _version_ | 1818808168511176704 |
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| author | Keiko Nishio Masanori Horie Yoko Akazawa Mototada Shichiri Hitoshi Iwahashi Yoshihisa Hagihara Yasukazu Yoshida Etsuo Niki |
| author_facet | Keiko Nishio Masanori Horie Yoko Akazawa Mototada Shichiri Hitoshi Iwahashi Yoshihisa Hagihara Yasukazu Yoshida Etsuo Niki |
| author_sort | Keiko Nishio |
| collection | DOAJ |
| description | Lipopolysaccharide (LPS) induces host inflammatory responses and tissue injury and has been implicated in the pathogenesis of various age-related diseases such as acute respiratory distress syndrome, vascular diseases, and periodontal disease. Antioxidants, particularly vitamin E, have been shown to suppress oxidative stress induced by LPS, but the previous studies with different vitamin E isoforms gave inconsistent results. In the present study, the protective effects of α- and γ-tocopherols and α- and γ-tocotrienols on the oxidative stress induced by LPS against human lung carcinoma A549 cells were studied. They suppressed intracellular reactive oxygen formation, lipid peroxidation, induction of inflammatory mediator cytokines, and cell death. Tocopherols were incorporated into cultured cells much slower than tocotrienols but could suppress LPS-induced oxidative stress at much lower intracellular concentration than tocotrienols. Considering the bioavailability, it was concluded that α-tocopherol may exhibit the highest protective capacity among the vitamin E isoforms against LPS-induced oxidative stress. |
| first_indexed | 2024-12-18T19:37:16Z |
| format | Article |
| id | doaj.art-24d05bc2ea9d4b678a79067c7c5a0cb6 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-12-18T19:37:16Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-24d05bc2ea9d4b678a79067c7c5a0cb62022-12-21T20:55:34ZengElsevierRedox Biology2213-23172013-01-01119710310.1016/j.redox.2012.10.002Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienolsKeiko NishioMasanori HorieYoko AkazawaMototada ShichiriHitoshi IwahashiYoshihisa HagiharaYasukazu YoshidaEtsuo NikiLipopolysaccharide (LPS) induces host inflammatory responses and tissue injury and has been implicated in the pathogenesis of various age-related diseases such as acute respiratory distress syndrome, vascular diseases, and periodontal disease. Antioxidants, particularly vitamin E, have been shown to suppress oxidative stress induced by LPS, but the previous studies with different vitamin E isoforms gave inconsistent results. In the present study, the protective effects of α- and γ-tocopherols and α- and γ-tocotrienols on the oxidative stress induced by LPS against human lung carcinoma A549 cells were studied. They suppressed intracellular reactive oxygen formation, lipid peroxidation, induction of inflammatory mediator cytokines, and cell death. Tocopherols were incorporated into cultured cells much slower than tocotrienols but could suppress LPS-induced oxidative stress at much lower intracellular concentration than tocotrienols. Considering the bioavailability, it was concluded that α-tocopherol may exhibit the highest protective capacity among the vitamin E isoforms against LPS-induced oxidative stress.http://www.sciencedirect.com/science/article/pii/S2213231712000122Lipid peroxidationLipopolysaccharide (LPS)Oxidative stressTocopherolTocotrienolVitamin E |
| spellingShingle | Keiko Nishio Masanori Horie Yoko Akazawa Mototada Shichiri Hitoshi Iwahashi Yoshihisa Hagihara Yasukazu Yoshida Etsuo Niki Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols Redox Biology Lipid peroxidation Lipopolysaccharide (LPS) Oxidative stress Tocopherol Tocotrienol Vitamin E |
| title | Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols |
| title_full | Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols |
| title_fullStr | Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols |
| title_full_unstemmed | Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols |
| title_short | Attenuation of lipopolysaccharide (LPS)-induced cytotoxicity by tocopherols and tocotrienols |
| title_sort | attenuation of lipopolysaccharide lps induced cytotoxicity by tocopherols and tocotrienols |
| topic | Lipid peroxidation Lipopolysaccharide (LPS) Oxidative stress Tocopherol Tocotrienol Vitamin E |
| url | http://www.sciencedirect.com/science/article/pii/S2213231712000122 |
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