Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla
Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in human dental pulp stem cells (hDPSCs). This study aimed to investigate the characteristics of the Jagged1-treated hDPSC-derived decellularized extracellular matrix (dECM) and its biological activity on odon...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.948812/full |
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| author | Suphalak Phothichailert Nunthawan Nowwarote Nunthawan Nowwarote Benjamin P.J. Fournier Benjamin P.J. Fournier Vorapat Trachoo Sittiruk Roytrakul Worachat Namangkalakul Thanaphum Osathanon Thanaphum Osathanon |
| author_facet | Suphalak Phothichailert Nunthawan Nowwarote Nunthawan Nowwarote Benjamin P.J. Fournier Benjamin P.J. Fournier Vorapat Trachoo Sittiruk Roytrakul Worachat Namangkalakul Thanaphum Osathanon Thanaphum Osathanon |
| author_sort | Suphalak Phothichailert |
| collection | DOAJ |
| description | Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in human dental pulp stem cells (hDPSCs). This study aimed to investigate the characteristics of the Jagged1-treated hDPSC-derived decellularized extracellular matrix (dECM) and its biological activity on odonto/osteogenic differentiation of stem cells isolated from apical papilla (SCAPs).Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed using NetworkAnalyst. hDPSCs seeded on the Jagged1 immobilized surface were maintained with normal or osteogenic induction medium (OM) followed by decellularization procedure, dECM-N, or dECM-OM, respectively. SCAPs were reseeded on each dECM with either the normal medium or the OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining, and alcian blue staining. Mineralization was assessed by alizarin red S, Von Kossa, and alkaline phosphatase staining. Statistical significance was considered at p < 0.05.Results: RNA-seq database revealed upregulation of several genes involved in ECM organization, ECM–receptor interaction, and focal adhesion in Jagged1-treated hDPSCs. Immobilized Jagged1 increased the osteogenesis of the hDPSC culture with OM. dECMs showed fibrillar-like network structure and maintained major ECM proteins, fibronectin, type I-collagen, and glycosaminoglycans. A decrease in calcium and phosphate components was observed in dECMs after the decellularized process. Cell viability on dECMs did not alter by 7 days. Cell attachment and f-actin cytoskeletal organization of SCAPs proliferated on Jagged1-treated dECMs were comparable to those of the control dECMs. SCAPs exhibited significantly higher mineralization on dECM-N in OM and markedly enhanced on dECM-OM with normal medium or OM conditions.Conclusion: Jagged1-treated hDPSC-derived dECMs are biocompatible and increase odonto/osteogenic differentiation of SCAPs. The results suggested the potential of Jagged1 dECMs, which could be further developed into ECM scaffolds for application in regenerative medicine. |
| first_indexed | 2024-12-11T00:44:45Z |
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| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-11T00:44:45Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-24d4b987f0e2412485ed3c351d585b472022-12-22T01:26:49ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-08-011010.3389/fcell.2022.948812948812Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papillaSuphalak Phothichailert0Nunthawan Nowwarote1Nunthawan Nowwarote2Benjamin P.J. Fournier3Benjamin P.J. Fournier4Vorapat Trachoo5Sittiruk Roytrakul6Worachat Namangkalakul7Thanaphum Osathanon8Thanaphum Osathanon9Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandUniversite Paris Cite, Faculty of Dentistry, Department of Oral Biology, Paris, FranceCentre de Recherche des Cordeliers, Sorbonne Universite, INSERM UMRS, Molecular Oral Pathophysiology, Paris, FranceUniversite Paris Cite, Faculty of Dentistry, Department of Oral Biology, Paris, FranceCentre de Recherche des Cordeliers, Sorbonne Universite, INSERM UMRS, Molecular Oral Pathophysiology, Paris, FranceDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandProteomics Research Laboratory, Genome Institute, National Center of Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, ThailandDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandDental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandObjective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in human dental pulp stem cells (hDPSCs). This study aimed to investigate the characteristics of the Jagged1-treated hDPSC-derived decellularized extracellular matrix (dECM) and its biological activity on odonto/osteogenic differentiation of stem cells isolated from apical papilla (SCAPs).Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed using NetworkAnalyst. hDPSCs seeded on the Jagged1 immobilized surface were maintained with normal or osteogenic induction medium (OM) followed by decellularization procedure, dECM-N, or dECM-OM, respectively. SCAPs were reseeded on each dECM with either the normal medium or the OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining, and alcian blue staining. Mineralization was assessed by alizarin red S, Von Kossa, and alkaline phosphatase staining. Statistical significance was considered at p < 0.05.Results: RNA-seq database revealed upregulation of several genes involved in ECM organization, ECM–receptor interaction, and focal adhesion in Jagged1-treated hDPSCs. Immobilized Jagged1 increased the osteogenesis of the hDPSC culture with OM. dECMs showed fibrillar-like network structure and maintained major ECM proteins, fibronectin, type I-collagen, and glycosaminoglycans. A decrease in calcium and phosphate components was observed in dECMs after the decellularized process. Cell viability on dECMs did not alter by 7 days. Cell attachment and f-actin cytoskeletal organization of SCAPs proliferated on Jagged1-treated dECMs were comparable to those of the control dECMs. SCAPs exhibited significantly higher mineralization on dECM-N in OM and markedly enhanced on dECM-OM with normal medium or OM conditions.Conclusion: Jagged1-treated hDPSC-derived dECMs are biocompatible and increase odonto/osteogenic differentiation of SCAPs. The results suggested the potential of Jagged1 dECMs, which could be further developed into ECM scaffolds for application in regenerative medicine.https://www.frontiersin.org/articles/10.3389/fcell.2022.948812/fullbiocompatibilitydecellularizeddifferentiationJagged1stem cells isolated from apical papilla |
| spellingShingle | Suphalak Phothichailert Nunthawan Nowwarote Nunthawan Nowwarote Benjamin P.J. Fournier Benjamin P.J. Fournier Vorapat Trachoo Sittiruk Roytrakul Worachat Namangkalakul Thanaphum Osathanon Thanaphum Osathanon Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla Frontiers in Cell and Developmental Biology biocompatibility decellularized differentiation Jagged1 stem cells isolated from apical papilla |
| title | Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| title_full | Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| title_fullStr | Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| title_full_unstemmed | Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| title_short | Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| title_sort | effects of decellularized extracellular matrix derived from jagged1 treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla |
| topic | biocompatibility decellularized differentiation Jagged1 stem cells isolated from apical papilla |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.948812/full |
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