Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn
Sinus gland from Penaeus merguiensis De man, 1888 was extracted and proteins were fractionated on a HPLC column (μBondapack-phenyl column, 9 mm i.d., 10 μm particle size, Waters). Three major peaks (peaks 3, 5 and 6) were collected and investigated for crustacean hyperglycemic hormone (CHH) and gona...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2005-12-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/27_openweek_suppl3_pdf/16_Banana_prawn.pdf |
| _version_ | 1818134658969239552 |
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| author | Wilaiwan Chotigeat Ausa Chanumpai Amornrat Phongdara Parin Wongsawang |
| author_facet | Wilaiwan Chotigeat Ausa Chanumpai Amornrat Phongdara Parin Wongsawang |
| author_sort | Wilaiwan Chotigeat |
| collection | DOAJ |
| description | Sinus gland from Penaeus merguiensis De man, 1888 was extracted and proteins were fractionated on a HPLC column (μBondapack-phenyl column, 9 mm i.d., 10 μm particle size, Waters). Three major peaks (peaks 3, 5 and 6) were collected and investigated for crustacean hyperglycemic hormone (CHH) and gonad inhibiting hormone activity (GIH). The CHH activity of the peak 5 fraction was significantly higher than the control while peak 6 did not have significant CHH activity. No CHH activity was found in the peak 3 fraction. GIH activity was determined by inhibition of total protein and vitellin synthesis in ovarian tissue. An antivitellin antibody was prepared and used for immunoprecipitation in the GIH activity assay. Hence GIH activity was detected in peaks 5 and 6 while peak 3 had gonad stimulating hormone like activity (GSH-like activity). |
| first_indexed | 2024-12-11T09:12:08Z |
| format | Article |
| id | doaj.art-24d5cf967c114106a47995c2d4bbc7d8 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-11T09:12:08Z |
| publishDate | 2005-12-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-24d5cf967c114106a47995c2d4bbc7d82022-12-22T01:13:27ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952005-12-0127Suppl.3789798Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawnWilaiwan ChotigeatAusa ChanumpaiAmornrat PhongdaraParin WongsawangSinus gland from Penaeus merguiensis De man, 1888 was extracted and proteins were fractionated on a HPLC column (μBondapack-phenyl column, 9 mm i.d., 10 μm particle size, Waters). Three major peaks (peaks 3, 5 and 6) were collected and investigated for crustacean hyperglycemic hormone (CHH) and gonad inhibiting hormone activity (GIH). The CHH activity of the peak 5 fraction was significantly higher than the control while peak 6 did not have significant CHH activity. No CHH activity was found in the peak 3 fraction. GIH activity was determined by inhibition of total protein and vitellin synthesis in ovarian tissue. An antivitellin antibody was prepared and used for immunoprecipitation in the GIH activity assay. Hence GIH activity was detected in peaks 5 and 6 while peak 3 had gonad stimulating hormone like activity (GSH-like activity).http://www.sjst.psu.ac.th/journal/27_openweek_suppl3_pdf/16_Banana_prawn.pdfanti-vitellincrustacean hyperglycemic hormone (CHH)gonad inhibiting hormone activity (GIH)ovaryPenaeus merguiensisprawnvitellin |
| spellingShingle | Wilaiwan Chotigeat Ausa Chanumpai Amornrat Phongdara Parin Wongsawang Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn Songklanakarin Journal of Science and Technology (SJST) anti-vitellin crustacean hyperglycemic hormone (CHH) gonad inhibiting hormone activity (GIH) ovary Penaeus merguiensis prawn vitellin |
| title | Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn |
| title_full | Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn |
| title_fullStr | Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn |
| title_full_unstemmed | Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn |
| title_short | Detection of CHH/GIH activity in fractionated extracts from the eyestalk of Banana prawn |
| title_sort | detection of chh gih activity in fractionated extracts from the eyestalk of banana prawn |
| topic | anti-vitellin crustacean hyperglycemic hormone (CHH) gonad inhibiting hormone activity (GIH) ovary Penaeus merguiensis prawn vitellin |
| url | http://www.sjst.psu.ac.th/journal/27_openweek_suppl3_pdf/16_Banana_prawn.pdf |
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