Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr.
Female sterility is a common phenomenon in the plant world, and systematic research has not been carried out in gymnosperms. In this study, the ovules of No. 28 sterile line and No. 15 fertile line <i>Pinus tabuliformis</i> were used as materials, and a total of 18 cDNA libraries were se...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1915 |
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| author | Zaixin Gong Hailin Hu Li Xu Yuanyuan Zhao Caixia Zheng |
| author_facet | Zaixin Gong Hailin Hu Li Xu Yuanyuan Zhao Caixia Zheng |
| author_sort | Zaixin Gong |
| collection | DOAJ |
| description | Female sterility is a common phenomenon in the plant world, and systematic research has not been carried out in gymnosperms. In this study, the ovules of No. 28 sterile line and No. 15 fertile line <i>Pinus tabuliformis</i> were used as materials, and a total of 18 cDNA libraries were sequenced by the HiSeqTM 4000 platform to analyze the differentially expressed genes (DEGs) and simple sequence repeats (SSRs) between the two lines. In addition, this study further analyzed the DEGs involved in the signal transduction of plant hormones, revealing that the signal pathways related to auxin, cytokinin, and gibberellin were blocked in the sterile ovule. Additionally, real-time fluorescent quantitative PCR verified that the expression trend of DEGs related to plant hormones was consistent with the results of high-throughput sequencing. Frozen sections and fluorescence in situ hybridization (FISH) were used to study the temporal and spatial expression patterns of <i>PtRab</i> in the ovules of <i>P. tabuliformis</i>. It was found that <i>PtRab</i> was significantly expressed in female gametophytes and rarely expressed in the surrounding diploid tissues. This study further explained the molecular regulation mechanism of female sterility in <i>P. tabuliformis</i>, preliminarily mining the key factors of ovule abortion in gymnosperms at the transcriptional level. |
| first_indexed | 2024-03-09T23:42:38Z |
| format | Article |
| id | doaj.art-24d629bd62a644e194866d2a0d0be400 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:42:38Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-24d629bd62a644e194866d2a0d0be4002023-11-23T16:48:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01233191510.3390/ijms23031915Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr.Zaixin Gong0Hailin Hu1Li Xu2Yuanyuan Zhao3Caixia Zheng4College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaFemale sterility is a common phenomenon in the plant world, and systematic research has not been carried out in gymnosperms. In this study, the ovules of No. 28 sterile line and No. 15 fertile line <i>Pinus tabuliformis</i> were used as materials, and a total of 18 cDNA libraries were sequenced by the HiSeqTM 4000 platform to analyze the differentially expressed genes (DEGs) and simple sequence repeats (SSRs) between the two lines. In addition, this study further analyzed the DEGs involved in the signal transduction of plant hormones, revealing that the signal pathways related to auxin, cytokinin, and gibberellin were blocked in the sterile ovule. Additionally, real-time fluorescent quantitative PCR verified that the expression trend of DEGs related to plant hormones was consistent with the results of high-throughput sequencing. Frozen sections and fluorescence in situ hybridization (FISH) were used to study the temporal and spatial expression patterns of <i>PtRab</i> in the ovules of <i>P. tabuliformis</i>. It was found that <i>PtRab</i> was significantly expressed in female gametophytes and rarely expressed in the surrounding diploid tissues. This study further explained the molecular regulation mechanism of female sterility in <i>P. tabuliformis</i>, preliminarily mining the key factors of ovule abortion in gymnosperms at the transcriptional level.https://www.mdpi.com/1422-0067/23/3/1915<i>Pinus tabuliformis</i>female infertilitytranscriptome sequencingplant hormonesfluorescence in situ hybridization (FISH) |
| spellingShingle | Zaixin Gong Hailin Hu Li Xu Yuanyuan Zhao Caixia Zheng Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. International Journal of Molecular Sciences <i>Pinus tabuliformis</i> female infertility transcriptome sequencing plant hormones fluorescence in situ hybridization (FISH) |
| title | Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. |
| title_full | Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. |
| title_fullStr | Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. |
| title_full_unstemmed | Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. |
| title_short | Screening of Differentially Expressed Genes and Localization Analysis of Female Gametophyte at the Free Nuclear Mitosis Stage in <i>Pinus tabuliformis</i> Carr. |
| title_sort | screening of differentially expressed genes and localization analysis of female gametophyte at the free nuclear mitosis stage in i pinus tabuliformis i carr |
| topic | <i>Pinus tabuliformis</i> female infertility transcriptome sequencing plant hormones fluorescence in situ hybridization (FISH) |
| url | https://www.mdpi.com/1422-0067/23/3/1915 |
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