Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia
Ventilator associated pneumonia (VAP) caused by P. aeruginosa is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the investigation of novel therapies. Specific polyclonal anti- P. aeruginosa IgY-antibodies ( Pa- IgY) migh...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-10-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/17534259221114217 |
| _version_ | 1797939006054334464 |
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| author | A. Otterbeck P. Skorup K. Hanslin A. Larsson J. Stålberg H. Hjelmqvist M. Lipcsey |
| author_facet | A. Otterbeck P. Skorup K. Hanslin A. Larsson J. Stålberg H. Hjelmqvist M. Lipcsey |
| author_sort | A. Otterbeck |
| collection | DOAJ |
| description | Ventilator associated pneumonia (VAP) caused by P. aeruginosa is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the investigation of novel therapies. Specific polyclonal anti- P. aeruginosa IgY-antibodies ( Pa- IgY) might be effective for VAP caused by P. aeruginosa. The objective of this study was to investigate if intravenous Pa- IgY decreases the lower airway concentration of P. aeruginosa in VAP. We used a double blind randomized placebo controlled porcine model of VAP caused by P. aeruginosa . Eighteen pigs were randomized to either receive intravenous Pa- IgY or placebo. Repeated registration of physiological parameters and sampling was performed for 27 h. Concentration of P. aeruginosa in BAL-cultures was similar in both groups with 10 4.97 ± 10 2.09 CFU/mL in the intervention group vs 10 4.37 ± 10 2.62 CFU/mL in the control group at the end of the experiment. The intervention group had higher heart rate, cardiac index, oxygen delivery and arterial oxygen tension/fraction of inspired oxygen-ratio, but lower plasma lactate and blood hemoglobin levels than the control group. In summary, in an anesthetized and mechanically ventilated porcine model of VAP, Pa- IgY at the dose used did not decrease concentrations of P. aeruginosa in the lower airways. |
| first_indexed | 2024-04-10T19:08:34Z |
| format | Article |
| id | doaj.art-24d64c34b72645f491817081f2daa241 |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-04-10T19:08:34Z |
| publishDate | 2022-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-24d64c34b72645f491817081f2daa2412023-01-30T19:44:56ZengSAGE PublishingInnate Immunity1753-42591753-42672022-10-012810.1177/17534259221114217Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumoniaA. Otterbeck0P. Skorup1K. Hanslin2A. Larsson3J. Stålberg4H. Hjelmqvist5M. Lipcsey6 Anesthesiology and Intensive Care, , Uppsala University, Uppsala, Sweden Section of Infectious Diseases, , Uppsala University, Uppsala, Sweden Anesthesiology and Intensive Care, , Uppsala University, Uppsala, Sweden Section of Clinical Chemistry, , Uppsala University, Uppsala, Sweden Section of Clinical Chemistry, , Uppsala University, Uppsala, Sweden Anesthesiology and Intensive Care, School of Medical Sciences, , Örebro, Sweden Hedenstierna laboratory, Department of Surgical Sciences, , Uppsala, SwedenVentilator associated pneumonia (VAP) caused by P. aeruginosa is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the investigation of novel therapies. Specific polyclonal anti- P. aeruginosa IgY-antibodies ( Pa- IgY) might be effective for VAP caused by P. aeruginosa. The objective of this study was to investigate if intravenous Pa- IgY decreases the lower airway concentration of P. aeruginosa in VAP. We used a double blind randomized placebo controlled porcine model of VAP caused by P. aeruginosa . Eighteen pigs were randomized to either receive intravenous Pa- IgY or placebo. Repeated registration of physiological parameters and sampling was performed for 27 h. Concentration of P. aeruginosa in BAL-cultures was similar in both groups with 10 4.97 ± 10 2.09 CFU/mL in the intervention group vs 10 4.37 ± 10 2.62 CFU/mL in the control group at the end of the experiment. The intervention group had higher heart rate, cardiac index, oxygen delivery and arterial oxygen tension/fraction of inspired oxygen-ratio, but lower plasma lactate and blood hemoglobin levels than the control group. In summary, in an anesthetized and mechanically ventilated porcine model of VAP, Pa- IgY at the dose used did not decrease concentrations of P. aeruginosa in the lower airways.https://doi.org/10.1177/17534259221114217 |
| spellingShingle | A. Otterbeck P. Skorup K. Hanslin A. Larsson J. Stålberg H. Hjelmqvist M. Lipcsey Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia Innate Immunity |
| title | Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia |
| title_full | Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia |
| title_fullStr | Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia |
| title_full_unstemmed | Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia |
| title_short | Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia |
| title_sort | intravenous anti igy antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator associated pneumonia |
| url | https://doi.org/10.1177/17534259221114217 |
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