Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome
Defects in mitochondrial ribosomal proteins (MRPs) cause various diseases in humans. Because of the essential role of MRPs in synthesizing the essential subunits of oxidative phosphorylation (OXPHOS) complexes, identifying all of the protein components involved in the mitochondrial translational ma...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00183/full |
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| author | Emine C Koc Huseyin eCimen Beril eKumcuoglu Nadiah eAbu Gurler eAkpinar Md Emdadul Haque Linda L Spremulli Hasan eKoc |
| author_facet | Emine C Koc Huseyin eCimen Beril eKumcuoglu Nadiah eAbu Gurler eAkpinar Md Emdadul Haque Linda L Spremulli Hasan eKoc |
| author_sort | Emine C Koc |
| collection | DOAJ |
| description | Defects in mitochondrial ribosomal proteins (MRPs) cause various diseases in humans. Because of the essential role of MRPs in synthesizing the essential subunits of oxidative phosphorylation (OXPHOS) complexes, identifying all of the protein components involved in the mitochondrial translational machinery is critical. Initially, we identified 79 MRPs; however, identifying MRPs with no clear homologs in bacteria and yeast mitochondria was challenging, due to limited availability of expressed sequence tags (ESTs) in the databases available at that time. With the improvement in genome sequencing and increased sensitivity of mass spectrometry (MS)-based technologies, we have established that four previously known proteins as MRPs and have confirmed the identification of ICT1 (MRP58) as a ribosomal protein. The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in the large subunit. Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins. |
| first_indexed | 2024-12-10T21:29:22Z |
| format | Article |
| id | doaj.art-24dce0ad3e054c9485d8fa20b4af5c10 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T21:29:22Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-24dce0ad3e054c9485d8fa20b4af5c102022-12-22T01:32:53ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2013-07-01410.3389/fphys.2013.0018348292Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial RibosomeEmine C Koc0Huseyin eCimen1Beril eKumcuoglu2Nadiah eAbu3Gurler eAkpinar4Md Emdadul Haque5Linda L Spremulli6Hasan eKoc7Marshall University School of MedicineMarshall University School of MedicinePennsylvania State UniversityPennsylvania State UniversityPennsylvania State UniversityUniversity of North CarolinaUniversity of North CarolinaMarshall University School of PharmacyDefects in mitochondrial ribosomal proteins (MRPs) cause various diseases in humans. Because of the essential role of MRPs in synthesizing the essential subunits of oxidative phosphorylation (OXPHOS) complexes, identifying all of the protein components involved in the mitochondrial translational machinery is critical. Initially, we identified 79 MRPs; however, identifying MRPs with no clear homologs in bacteria and yeast mitochondria was challenging, due to limited availability of expressed sequence tags (ESTs) in the databases available at that time. With the improvement in genome sequencing and increased sensitivity of mass spectrometry (MS)-based technologies, we have established that four previously known proteins as MRPs and have confirmed the identification of ICT1 (MRP58) as a ribosomal protein. The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in the large subunit. Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins.http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00183/fullMitochondrial translationMRPS37 (CHCHD1)MRPS38 (AURKAIP1)MRPS39 (PTCD3)MRPL58 (ICT1)MRPL59 (CRIF1) |
| spellingShingle | Emine C Koc Huseyin eCimen Beril eKumcuoglu Nadiah eAbu Gurler eAkpinar Md Emdadul Haque Linda L Spremulli Hasan eKoc Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome Frontiers in Physiology Mitochondrial translation MRPS37 (CHCHD1) MRPS38 (AURKAIP1) MRPS39 (PTCD3) MRPL58 (ICT1) MRPL59 (CRIF1) |
| title | Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome |
| title_full | Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome |
| title_fullStr | Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome |
| title_full_unstemmed | Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome |
| title_short | Identification and Characterization of CHCHD1, AURKAIP1, and CRIF1 as New Members of the Mammalian Mitochondrial Ribosome |
| title_sort | identification and characterization of chchd1 aurkaip1 and crif1 as new members of the mammalian mitochondrial ribosome |
| topic | Mitochondrial translation MRPS37 (CHCHD1) MRPS38 (AURKAIP1) MRPS39 (PTCD3) MRPL58 (ICT1) MRPL59 (CRIF1) |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2013.00183/full |
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