Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons

Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neurons

Summary: Genetic variants that affect neurological function will often produce changes visible at the level of gross morphology, either of the whole brain or of specific neuronal types. Here we describe how to perfuse and dissect the brain in preparation for Nissl staining. Then we outline steps for...

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Bibliographic Details
Main Authors: Nicola de Prisco, Alexei Chemiakine, Winston Lee, Salvatore Botta, Vincenzo A. Gennarino
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Cell-based Assays
Genetics
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001241

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