De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition
β-Poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in food, medicine and other industries. To date, the biosynthesis pathway of PMLA has not been fully elucidated. In this study, we sequenced the transcriptome of strain Aureobasidium melanogenum under 20 g/L CaCO3 addition. The resulting...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002269 |
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| author | Genan Wang Haisong Yin Tingbin Zhao Donglin Yang Shiru Jia Changsheng Qiao |
| author_facet | Genan Wang Haisong Yin Tingbin Zhao Donglin Yang Shiru Jia Changsheng Qiao |
| author_sort | Genan Wang |
| collection | DOAJ |
| description | β-Poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in food, medicine and other industries. To date, the biosynthesis pathway of PMLA has not been fully elucidated. In this study, we sequenced the transcriptome of strain Aureobasidium melanogenum under 20 g/L CaCO3 addition. The resulting sequencing reads were assembled and annotated for the differentially expressed genes (DEGs) analysis and novel transcripts identification. The result indicated that with the CaCO3 addition, the tricarboxylic cycle (TCA) cycle and glyoxylate pathway were up-regulated, and it also found that a non-ribosomal peptide synthetase (NRPS) like protein was highly expressed. The DEGs analysis showed a high expression level of malate dehydrogenase (MDHC) and phosphoenolpyruvate carboxykinase (PCKA) in the CaCO3 group, which indicated a cytosolic malate activity. We speculated that the malate should be transported to or synthesized in the cytoplasm, which was then polymerized to PMLA by the NRPS-like protein, accompanied by the up-regulated TCA cycle providing ATP for the polymerization. Depending on the analysis, we assumed that an NRPS-like protein, the TCA cycle, and the cytosolic malate together are contributing to the PMLA biosynthesis. |
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| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2025-02-16T09:26:27Z |
| publishDate | 2023-07-01 |
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| spelling | doaj.art-24e1a2d0c51c43b99ae39bee987be8952025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412481256De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 additionGenan Wang0Haisong Yin1Tingbin Zhao2Donglin Yang3Shiru Jia4Changsheng Qiao5Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; School of Bioengineering, Tianjin Modern Vocational Technology College, Tianjin 300350, ChinaTianjin Huizhi Biotrans Bioengineering Co., Ltd., Tianjin 300457, ChinaKey Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaKey Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Corresponding authors at: Tianjin University of Science and Technology, Tianjin 300457, China.Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457,China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Huizhi Biotrans Bioengineering Co., Ltd., Tianjin 300457, China; Corresponding authors at: Tianjin University of Science and Technology, Tianjin 300457, China.β-Poly(L-malic acid) (PMLA) is a water-soluble biopolymer used in food, medicine and other industries. To date, the biosynthesis pathway of PMLA has not been fully elucidated. In this study, we sequenced the transcriptome of strain Aureobasidium melanogenum under 20 g/L CaCO3 addition. The resulting sequencing reads were assembled and annotated for the differentially expressed genes (DEGs) analysis and novel transcripts identification. The result indicated that with the CaCO3 addition, the tricarboxylic cycle (TCA) cycle and glyoxylate pathway were up-regulated, and it also found that a non-ribosomal peptide synthetase (NRPS) like protein was highly expressed. The DEGs analysis showed a high expression level of malate dehydrogenase (MDHC) and phosphoenolpyruvate carboxykinase (PCKA) in the CaCO3 group, which indicated a cytosolic malate activity. We speculated that the malate should be transported to or synthesized in the cytoplasm, which was then polymerized to PMLA by the NRPS-like protein, accompanied by the up-regulated TCA cycle providing ATP for the polymerization. Depending on the analysis, we assumed that an NRPS-like protein, the TCA cycle, and the cytosolic malate together are contributing to the PMLA biosynthesis.http://www.sciencedirect.com/science/article/pii/S2213453022002269De novo transcriptome analysisβ-Poly(L-malic acid)Aureobasidium melanogenum |
| spellingShingle | Genan Wang Haisong Yin Tingbin Zhao Donglin Yang Shiru Jia Changsheng Qiao De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition Food Science and Human Wellness De novo transcriptome analysis β-Poly(L-malic acid) Aureobasidium melanogenum |
| title | De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition |
| title_full | De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition |
| title_fullStr | De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition |
| title_full_unstemmed | De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition |
| title_short | De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze the β-poly(L-malic acid) biosynthesis pathway under the CaCO3 addition |
| title_sort | de novo transcriptome assembly of aureobasidium melanogenum cgmcc18996 to analyze the β poly l malic acid biosynthesis pathway under the caco3 addition |
| topic | De novo transcriptome analysis β-Poly(L-malic acid) Aureobasidium melanogenum |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002269 |
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