DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region
Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to charac...
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| Format: | Article |
| Language: | English |
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Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya
2022-09-01
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| Series: | Jurnal Riset Biologi dan Aplikasinya |
| Subjects: | |
| Online Access: | https://journal.unesa.ac.id/index.php/risetbiologi/article/view/18920 |
| _version_ | 1811213534764204032 |
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| author | Risqi Aprilianingsih Baiq Farhatul Wahidah Muhammad Rifqi Hariri |
| author_facet | Risqi Aprilianingsih Baiq Farhatul Wahidah Muhammad Rifqi Hariri |
| author_sort | Risqi Aprilianingsih |
| collection | DOAJ |
| description | Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species. |
| first_indexed | 2024-04-12T05:48:09Z |
| format | Article |
| id | doaj.art-24e39817619a460d892c32d901d8b8a8 |
| institution | Directory Open Access Journal |
| issn | 2655-9927 |
| language | English |
| last_indexed | 2024-04-12T05:48:09Z |
| publishDate | 2022-09-01 |
| publisher | Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya |
| record_format | Article |
| series | Jurnal Riset Biologi dan Aplikasinya |
| spelling | doaj.art-24e39817619a460d892c32d901d8b8a82022-12-22T03:45:22ZengDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri SurabayaJurnal Riset Biologi dan Aplikasinya2655-99272022-09-0142697410.26740/jrba.v4n2.p69-7420571DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer RegionRisqi Aprilianingsih0Baiq Farhatul Wahidah1Muhammad Rifqi Hariri2Program Study of Biology, Faculty of Science and Technology, Walisongo State Islamic UniversityProgram Study of Biology, Faculty of Science and Technology, Walisongo State Islamic UniversityResearch Center for Biosystematics and Evolution - National Research and Innovation AgencyHomalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.https://journal.unesa.ac.id/index.php/risetbiologi/article/view/18920dna barcode, its, phylogenetic, homalomena pexa |
| spellingShingle | Risqi Aprilianingsih Baiq Farhatul Wahidah Muhammad Rifqi Hariri DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region Jurnal Riset Biologi dan Aplikasinya dna barcode, its, phylogenetic, homalomena pexa |
| title | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region |
| title_full | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region |
| title_fullStr | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region |
| title_full_unstemmed | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region |
| title_short | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region |
| title_sort | dna barcode of homalomena pexa inferred from internal transcribed spacer region |
| topic | dna barcode, its, phylogenetic, homalomena pexa |
| url | https://journal.unesa.ac.id/index.php/risetbiologi/article/view/18920 |
| work_keys_str_mv | AT risqiaprilianingsih dnabarcodeofhomalomenapexainferredfrominternaltranscribedspacerregion AT baiqfarhatulwahidah dnabarcodeofhomalomenapexainferredfrominternaltranscribedspacerregion AT muhammadrifqihariri dnabarcodeofhomalomenapexainferredfrominternaltranscribedspacerregion |