Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances
Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.891538/full |
| _version_ | 1817982552775852032 |
|---|---|
| author | Virginia Panara Rui Monteiro Rui Monteiro Katarzyna Koltowska |
| author_facet | Virginia Panara Rui Monteiro Rui Monteiro Katarzyna Koltowska |
| author_sort | Virginia Panara |
| collection | DOAJ |
| description | Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of cis-regulatory elements in vivo by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as gata2a, notch1b and dll4. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the cis-regulatory sequences that shape the zebrafish vascular network. |
| first_indexed | 2024-04-13T23:22:15Z |
| format | Article |
| id | doaj.art-24e3ad698a4245b5a69e9b4875d1214b |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T23:22:15Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-24e3ad698a4245b5a69e9b4875d1214b2022-12-22T02:25:11ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-05-011010.3389/fcell.2022.891538891538Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical AdvancesVirginia Panara0Rui Monteiro1Rui Monteiro2Katarzyna Koltowska3Immunology Genetics and Pathology, Uppsala University, Uppsala, SwedenInstitute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United KingdomBirmingham Centre of Genome Biology, University of Birmingham, Birmingham, United KingdomImmunology Genetics and Pathology, Uppsala University, Uppsala, SwedenEpigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of cis-regulatory elements in vivo by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as gata2a, notch1b and dll4. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the cis-regulatory sequences that shape the zebrafish vascular network.https://www.frontiersin.org/articles/10.3389/fcell.2022.891538/fullblood endothelial cellslymphatic endothelial cellsepigeneticsATAC-seqChIP-seqenhancers |
| spellingShingle | Virginia Panara Rui Monteiro Rui Monteiro Katarzyna Koltowska Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances Frontiers in Cell and Developmental Biology blood endothelial cells lymphatic endothelial cells epigenetics ATAC-seq ChIP-seq enhancers |
| title | Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances |
| title_full | Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances |
| title_fullStr | Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances |
| title_full_unstemmed | Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances |
| title_short | Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances |
| title_sort | epigenetic regulation of endothelial cell lineages during zebrafish development new insights from technical advances |
| topic | blood endothelial cells lymphatic endothelial cells epigenetics ATAC-seq ChIP-seq enhancers |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.891538/full |
| work_keys_str_mv | AT virginiapanara epigeneticregulationofendothelialcelllineagesduringzebrafishdevelopmentnewinsightsfromtechnicaladvances AT ruimonteiro epigeneticregulationofendothelialcelllineagesduringzebrafishdevelopmentnewinsightsfromtechnicaladvances AT ruimonteiro epigeneticregulationofendothelialcelllineagesduringzebrafishdevelopmentnewinsightsfromtechnicaladvances AT katarzynakoltowska epigeneticregulationofendothelialcelllineagesduringzebrafishdevelopmentnewinsightsfromtechnicaladvances |