Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period
Background Oral glucose loading may affect oxidative status during oral glucose tolerance test (OGTT). We aimed to investigate how oxidant and antioxidant markers and thiol/disulfide parameters change during OGTT. Methods OGTT was performed to 42 volunteers who were considered risk of type 2 diabete...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-07-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2022.2084189 |
| _version_ | 1827817119675318272 |
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| author | Zeynep Küskü Kiraz Ezgi Kar Fatih Kar Evin Kocatürk Medine Nur Kebapçı İ. Özkan Alataş Sema Uslu |
| author_facet | Zeynep Küskü Kiraz Ezgi Kar Fatih Kar Evin Kocatürk Medine Nur Kebapçı İ. Özkan Alataş Sema Uslu |
| author_sort | Zeynep Küskü Kiraz |
| collection | DOAJ |
| description | Background Oral glucose loading may affect oxidative status during oral glucose tolerance test (OGTT). We aimed to investigate how oxidant and antioxidant markers and thiol/disulfide parameters change during OGTT. Methods OGTT was performed to 42 volunteers who were considered risk of type 2 diabetes and were divided into three groups (normoglycemic, prediabetes, diabetes) according glucose levels during OGTT. Glucose, insulin, c-peptide, total antioxidant status (TAS), total oxidant status (TOS), total thiol and native thiol were investigated with auto-anaylzer for five-hours period. Results Decrease of TAS and increase of TOS levels began with the increase in glucose and insulin levels. The increase of TAS started at third hour and reached the highest levels at fifth hour. OSI levels were higher at fourth hour than fasting and first hours in normoglycemic and diabetes groups. In the prediabetic group, TAS were higher than the other groups, TOS peak was at the second hour (p < 0.05). Native thiol and total thiol levels showed variable course during OGTT, both parameters increased at the end of the process (p < 0.05). Disulfide levels showed an increase trend but it was not statistically different in normoglycemic and diabetes groups. In prediabetes group, second hour disulfide level was lower than fasting state and disulfide was significantly increased at third, fourth and fifth hours and fifth hour disulfide level was also higher than fasting. Conclusion Oxidative stress parameters and thiol/disulfide balance were found to deteriorate within five-hours after glucose loading in all groups. These results indicates that oxidative stress occurs during OGTT. |
| first_indexed | 2024-03-12T00:29:52Z |
| format | Article |
| id | doaj.art-24e3b2136334442a8a1c385d4437d3fe |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:29:52Z |
| publishDate | 2022-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-24e3b2136334442a8a1c385d4437d3fe2023-09-15T10:21:30ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532022-07-013581626163410.1080/08941939.2022.20841892084189Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour PeriodZeynep Küskü Kiraz0Ezgi Kar1Fatih Kar2Evin Kocatürk3Medine Nur Kebapçı4İ. Özkan Alataş5Sema Uslu6Faculty of Medicine Department of Medical Biochemistry, Eskişehir Osmangazi UniversityTraining and Research Center, Kütahya Health Science UniversityFaculty of Engineering and Natural Department of Basic Sciences, Kütahya Health Science UniversityFaculty of Medicine Department of Medical Biochemistry, Eskişehir Osmangazi UniversityFaculty of Medicine Department of Endocrinology Eskişehir Osmangazi University, EskişehirFaculty of Medicine Department of Medical Biochemistry, Eskişehir Osmangazi UniversityFaculty of Medicine Department of Medical Biochemistry, Eskişehir Osmangazi UniversityBackground Oral glucose loading may affect oxidative status during oral glucose tolerance test (OGTT). We aimed to investigate how oxidant and antioxidant markers and thiol/disulfide parameters change during OGTT. Methods OGTT was performed to 42 volunteers who were considered risk of type 2 diabetes and were divided into three groups (normoglycemic, prediabetes, diabetes) according glucose levels during OGTT. Glucose, insulin, c-peptide, total antioxidant status (TAS), total oxidant status (TOS), total thiol and native thiol were investigated with auto-anaylzer for five-hours period. Results Decrease of TAS and increase of TOS levels began with the increase in glucose and insulin levels. The increase of TAS started at third hour and reached the highest levels at fifth hour. OSI levels were higher at fourth hour than fasting and first hours in normoglycemic and diabetes groups. In the prediabetic group, TAS were higher than the other groups, TOS peak was at the second hour (p < 0.05). Native thiol and total thiol levels showed variable course during OGTT, both parameters increased at the end of the process (p < 0.05). Disulfide levels showed an increase trend but it was not statistically different in normoglycemic and diabetes groups. In prediabetes group, second hour disulfide level was lower than fasting state and disulfide was significantly increased at third, fourth and fifth hours and fifth hour disulfide level was also higher than fasting. Conclusion Oxidative stress parameters and thiol/disulfide balance were found to deteriorate within five-hours after glucose loading in all groups. These results indicates that oxidative stress occurs during OGTT.http://dx.doi.org/10.1080/08941939.2022.2084189oral]glucose tolerance testoxidative stresstotal antioxidant statustotal oxidant statusthiol-disulfide |
| spellingShingle | Zeynep Küskü Kiraz Ezgi Kar Fatih Kar Evin Kocatürk Medine Nur Kebapçı İ. Özkan Alataş Sema Uslu Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period Journal of Investigative Surgery oral] glucose tolerance test oxidative stress total antioxidant status total oxidant status thiol-disulfide |
| title | Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period |
| title_full | Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period |
| title_fullStr | Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period |
| title_full_unstemmed | Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period |
| title_short | Oxidative Status and Thiol/Disulfide Homeostasis Are Changed During 75 g Oral Glucose Tolerance Test over a Five-Hour Period |
| title_sort | oxidative status and thiol disulfide homeostasis are changed during 75 g oral glucose tolerance test over a five hour period |
| topic | oral] glucose tolerance test oxidative stress total antioxidant status total oxidant status thiol-disulfide |
| url | http://dx.doi.org/10.1080/08941939.2022.2084189 |
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