Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide)
The red palm weevil, Rhynchophorus ferrugineus (Olivier), is one of the most destructive pests of palm trees in the southeast of Iran. Digestion in the alimentary canal of the red palm weevil is facilitated by some carbohydrases. Results of the in vitro studies indicated the presence of α- and β-glu...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2012-06-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201202-0005_biochemical-characterisation-of-alpha-and-beta-glucosidases-and-alpha-and-beta-galactosidases-from-re.php |
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| author | Nasir Saberi RISEH Mohammad GHADAMYARI Behnam MOTAMEDINIYA |
| author_facet | Nasir Saberi RISEH Mohammad GHADAMYARI Behnam MOTAMEDINIYA |
| author_sort | Nasir Saberi RISEH |
| collection | DOAJ |
| description | The red palm weevil, Rhynchophorus ferrugineus (Olivier), is one of the most destructive pests of palm trees in the southeast of Iran. Digestion in the alimentary canal of the red palm weevil is facilitated by some carbohydrases. Results of the in vitro studies indicated the presence of α- and β-glucosidases and α- and β-galactosidases in the digestive system and haemolymph of this pest. In the digestive system of females, the activities of α-glucosidase and β-galactosidase were higher than those of β-glucosidase and α-galactosidase. Also, the specific activity of α- and β-glucosidases and α- and β-galactosidases in the female digestive system was much higher than that in larvae. Results showed that the highest activities of α- and β-glucosidases were at pH 5 and of α- and β-galactosidases at pH 4. The R. ferrugineus α- and β-glucosidases and α- and β-galactosidases have an optimum temperature activity at 50, 50-60, 40-60, and 40°C, respectively. A zymogram pattern in the native gel revealed that R. ferrugineus α- and β-glucosidases and α- and β-galactosidases in the digestive system showed 2, 3, 1 and 1 major bands, respectively. The activity of α-glucosidase in the digestive system of larvae and female adults was higher than that of the other carbohydrases. Therefore, it is the most important subject for further study and design of a new approach to the control of this pest using carbohydrase inhibitors. |
| first_indexed | 2024-04-10T08:14:53Z |
| format | Article |
| id | doaj.art-24e6cd04bb5e454fb8037998946ac3d7 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:14:53Z |
| publishDate | 2012-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-24e6cd04bb5e454fb8037998946ac3d72023-02-23T03:44:05ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412012-06-01482859310.17221/19/2011-PPSpps-201202-0005Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide)Nasir Saberi RISEH0Mohammad GHADAMYARI1Behnam MOTAMEDINIYA2Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, IranDepartment of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, IranAgricultural and Natural Resource Research Centre of Baluchestan, Baluchestan, IranThe red palm weevil, Rhynchophorus ferrugineus (Olivier), is one of the most destructive pests of palm trees in the southeast of Iran. Digestion in the alimentary canal of the red palm weevil is facilitated by some carbohydrases. Results of the in vitro studies indicated the presence of α- and β-glucosidases and α- and β-galactosidases in the digestive system and haemolymph of this pest. In the digestive system of females, the activities of α-glucosidase and β-galactosidase were higher than those of β-glucosidase and α-galactosidase. Also, the specific activity of α- and β-glucosidases and α- and β-galactosidases in the female digestive system was much higher than that in larvae. Results showed that the highest activities of α- and β-glucosidases were at pH 5 and of α- and β-galactosidases at pH 4. The R. ferrugineus α- and β-glucosidases and α- and β-galactosidases have an optimum temperature activity at 50, 50-60, 40-60, and 40°C, respectively. A zymogram pattern in the native gel revealed that R. ferrugineus α- and β-glucosidases and α- and β-galactosidases in the digestive system showed 2, 3, 1 and 1 major bands, respectively. The activity of α-glucosidase in the digestive system of larvae and female adults was higher than that of the other carbohydrases. Therefore, it is the most important subject for further study and design of a new approach to the control of this pest using carbohydrase inhibitors.https://pps.agriculturejournals.cz/artkey/pps-201202-0005_biochemical-characterisation-of-alpha-and-beta-glucosidases-and-alpha-and-beta-galactosidases-from-re.phpcarbohydratesdigestive systempalm trees |
| spellingShingle | Nasir Saberi RISEH Mohammad GHADAMYARI Behnam MOTAMEDINIYA Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) Plant Protection Science carbohydrates digestive system palm trees |
| title | Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) |
| title_full | Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) |
| title_fullStr | Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) |
| title_full_unstemmed | Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) |
| title_short | Biochemical characterisation of α- and β-glucosidases and α- and β-galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col.: Curculionide) |
| title_sort | biochemical characterisation of α and β glucosidases and α and β galactosidases from red palm weevil rhynchophorus ferrugineus olivier col curculionide |
| topic | carbohydrates digestive system palm trees |
| url | https://pps.agriculturejournals.cz/artkey/pps-201202-0005_biochemical-characterisation-of-alpha-and-beta-glucosidases-and-alpha-and-beta-galactosidases-from-re.php |
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