Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study
Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps gene...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-01-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6374/9/1/12 |
| _version_ | 1818489560627150848 |
|---|---|
| author | Helena Ukkonen Simo Vuokila Jopi J. W. Mikkonen Hannah Dekker Engelbert A. J. M. Schulten Elisabeth Bloemena Arto Koistinen Tulio A. Valdez Arja M. Kullaa Surya Pratap Singh |
| author_facet | Helena Ukkonen Simo Vuokila Jopi J. W. Mikkonen Hannah Dekker Engelbert A. J. M. Schulten Elisabeth Bloemena Arto Koistinen Tulio A. Valdez Arja M. Kullaa Surya Pratap Singh |
| author_sort | Helena Ukkonen |
| collection | DOAJ |
| description | Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy. |
| first_indexed | 2024-12-10T17:05:33Z |
| format | Article |
| id | doaj.art-24e84349c70f455c8d1ef08efc16044f |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-10T17:05:33Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-24e84349c70f455c8d1ef08efc16044f2022-12-22T01:40:27ZengMDPI AGBiosensors2079-63742019-01-01911210.3390/bios9010012bios9010012Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic StudyHelena Ukkonen0Simo Vuokila1Jopi J. W. Mikkonen2Hannah Dekker3Engelbert A. J. M. Schulten4Elisabeth Bloemena5Arto Koistinen6Tulio A. Valdez7Arja M. Kullaa8Surya Pratap Singh9Research Group of Oral Health Sciences, Faculty of Medicine, University of Oulu, and Oulu University Hospital, P.O. Box 8000, 90014 Oulu, FinlandSIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, FinlandSIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, FinlandAmsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The NetherlandsAmsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The NetherlandsAmsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Department of Oral and Maxillofacial Surgery/Oral Pathology, De Boelelaan 1117, 1081 HV Amsterdam, The NetherlandsSIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, FinlandDepartment of Otolaryngology, School of Medicine, Stanford University, Patlo Alto, CA 94305, USAResearch Group of Oral Health Sciences, Faculty of Medicine, University of Oulu, and Oulu University Hospital, P.O. Box 8000, 90014 Oulu, FinlandSIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, FinlandRadiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Spectra extracted from this layer suggests changes in overall nucleic acid and protein content in response to the therapeutic irradiation. Discrimination among control and irradiated groups have been achieved using principal component analysis. Findings of this preliminary study further support prospective utilization of Fourier Transform InfraRed (FTIR) imaging as a non-destructive, label-free tool for objective assessment of the oral mucosa in patient groups with or without radiation therapy.http://www.mdpi.com/2079-6374/9/1/12FTIR spectroscopyhead and neck cancerradiotherapy |
| spellingShingle | Helena Ukkonen Simo Vuokila Jopi J. W. Mikkonen Hannah Dekker Engelbert A. J. M. Schulten Elisabeth Bloemena Arto Koistinen Tulio A. Valdez Arja M. Kullaa Surya Pratap Singh Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study Biosensors FTIR spectroscopy head and neck cancer radiotherapy |
| title | Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study |
| title_full | Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study |
| title_fullStr | Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study |
| title_full_unstemmed | Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study |
| title_short | Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study |
| title_sort | biochemical changes in irradiated oral mucosa a ftir spectroscopic study |
| topic | FTIR spectroscopy head and neck cancer radiotherapy |
| url | http://www.mdpi.com/2079-6374/9/1/12 |
| work_keys_str_mv | AT helenaukkonen biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT simovuokila biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT jopijwmikkonen biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT hannahdekker biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT engelbertajmschulten biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT elisabethbloemena biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT artokoistinen biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT tulioavaldez biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT arjamkullaa biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy AT suryapratapsingh biochemicalchangesinirradiatedoralmucosaaftirspectroscopicstudy |