Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans
Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarct...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006991/full |
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| author | Shenghao Liu Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Bailin Cong Zhaohui Zhang Zhaohui Zhang Linlin Zhao Linlin Zhao |
| author_facet | Shenghao Liu Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Bailin Cong Zhaohui Zhang Zhaohui Zhang Linlin Zhao Linlin Zhao |
| author_sort | Shenghao Liu |
| collection | DOAJ |
| description | Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants. |
| first_indexed | 2024-04-12T21:55:56Z |
| format | Article |
| id | doaj.art-24eeed0268814e818badc347dc07f49c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T21:55:56Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-24eeed0268814e818badc347dc07f49c2022-12-22T03:15:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10069911006991Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutansShenghao Liu0Shenghao Liu1Tingting Li2Shuo Fang3Pengying Zhang4Dan Yi5Bailin Cong6Bailin Cong7Zhaohui Zhang8Zhaohui Zhang9Linlin Zhao10Linlin Zhao11Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaSchool of Advanced Manufacturing, Fuzhou University, Jinjiang, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaNational Glycoengineering Research Center, School of Life Sciences, Shandong University, Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaSchool of Advanced Manufacturing, Fuzhou University, Jinjiang, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaLaboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology, Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, ChinaSchool of Advanced Manufacturing, Fuzhou University, Jinjiang, ChinaAntarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.1006991/fullcold stressflavonoidsvery-long-chain fatty acidsmetabolomic profilingtranscriptomic sequencingbryophytes |
| spellingShingle | Shenghao Liu Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Bailin Cong Zhaohui Zhang Zhaohui Zhang Linlin Zhao Linlin Zhao Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans Frontiers in Plant Science cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes |
| title | Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
| title_full | Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
| title_fullStr | Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
| title_full_unstemmed | Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
| title_short | Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
| title_sort | metabolic profiling and gene expression analyses provide insights into cold adaptation of an antarctic moss pohlia nutans |
| topic | cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006991/full |
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