Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses
Abstract Background Cerebrospinal fluid (CSF) is highly labile and delayed processing might alter results of analysis. Hypothesis/Objectives To determine the effects of time and addition of autologous serum on cytological evaluation of CSF. Animals Ten client‐owned adult horses requiring euthanasia....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.16637 |
| _version_ | 1797856195566895104 |
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| author | Camilla Quattrini Rebeca Scalco William Vernau Pouya Dini Monica Aleman |
| author_facet | Camilla Quattrini Rebeca Scalco William Vernau Pouya Dini Monica Aleman |
| author_sort | Camilla Quattrini |
| collection | DOAJ |
| description | Abstract Background Cerebrospinal fluid (CSF) is highly labile and delayed processing might alter results of analysis. Hypothesis/Objectives To determine the effects of time and addition of autologous serum on cytological evaluation of CSF. Animals Ten client‐owned adult horses requiring euthanasia. Methods Prospective study. Serum and CSF were collected from each horse before and within 10 minutes after euthanasia. CSF samples were divided into 15 aliquots (2 mL each); 1 aliquot was submitted for routine CSF analysis within 60 minutes of collection. Four drops of autologous serum were added to 7 of the aliquots, and stored at 4°C (serum group); the remaining 7 samples were stored unaltered at 4°C (control group). Total nucleated cell count (TNCC) and cell morphology score were done at T4, T8, T12, T24, T48, T72, and T96 hours after collection. Protein concentration was measured in the control group at T0 and T96 hours. Results The cell morphology scores were significantly different in the control group at T48 (median 2, range 0‐4), T72 (2, 0‐4), and T96 (3, 0‐4) in comparison to T0 (1). No change was observed in the serum group. TNCC remained stable over time in both groups. No statistically significant difference in CSF protein concentration was found between T0 and T96. Conclusions and Clinical Importance The addition of autologous serum to an aliquot of CSF sample before shipping improves the preservation of cell morphology up to 96 hours after collection. |
| first_indexed | 2024-04-09T20:36:28Z |
| format | Article |
| id | doaj.art-24f246e2d2574baa8c697b6167e14545 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-09T20:36:28Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-24f246e2d2574baa8c697b6167e145452023-03-30T07:47:06ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-03-0137271371710.1111/jvim.16637Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horsesCamilla Quattrini0Rebeca Scalco1William Vernau2Pouya Dini3Monica Aleman4William R. Pritchard Veterinary Medical Teaching Hospital (Quatrini) and Department of Medicine and Epidemiology (Scalco, Aleman), Pathology, Microbiology and Immunology (Vernau), Population, Health and Reproduction (Dini), School of Veterinary Medicine University of California Davis Davis California USAWilliam R. Pritchard Veterinary Medical Teaching Hospital (Quatrini) and Department of Medicine and Epidemiology (Scalco, Aleman), Pathology, Microbiology and Immunology (Vernau), Population, Health and Reproduction (Dini), School of Veterinary Medicine University of California Davis Davis California USAWilliam R. Pritchard Veterinary Medical Teaching Hospital (Quatrini) and Department of Medicine and Epidemiology (Scalco, Aleman), Pathology, Microbiology and Immunology (Vernau), Population, Health and Reproduction (Dini), School of Veterinary Medicine University of California Davis Davis California USAWilliam R. Pritchard Veterinary Medical Teaching Hospital (Quatrini) and Department of Medicine and Epidemiology (Scalco, Aleman), Pathology, Microbiology and Immunology (Vernau), Population, Health and Reproduction (Dini), School of Veterinary Medicine University of California Davis Davis California USAWilliam R. Pritchard Veterinary Medical Teaching Hospital (Quatrini) and Department of Medicine and Epidemiology (Scalco, Aleman), Pathology, Microbiology and Immunology (Vernau), Population, Health and Reproduction (Dini), School of Veterinary Medicine University of California Davis Davis California USAAbstract Background Cerebrospinal fluid (CSF) is highly labile and delayed processing might alter results of analysis. Hypothesis/Objectives To determine the effects of time and addition of autologous serum on cytological evaluation of CSF. Animals Ten client‐owned adult horses requiring euthanasia. Methods Prospective study. Serum and CSF were collected from each horse before and within 10 minutes after euthanasia. CSF samples were divided into 15 aliquots (2 mL each); 1 aliquot was submitted for routine CSF analysis within 60 minutes of collection. Four drops of autologous serum were added to 7 of the aliquots, and stored at 4°C (serum group); the remaining 7 samples were stored unaltered at 4°C (control group). Total nucleated cell count (TNCC) and cell morphology score were done at T4, T8, T12, T24, T48, T72, and T96 hours after collection. Protein concentration was measured in the control group at T0 and T96 hours. Results The cell morphology scores were significantly different in the control group at T48 (median 2, range 0‐4), T72 (2, 0‐4), and T96 (3, 0‐4) in comparison to T0 (1). No change was observed in the serum group. TNCC remained stable over time in both groups. No statistically significant difference in CSF protein concentration was found between T0 and T96. Conclusions and Clinical Importance The addition of autologous serum to an aliquot of CSF sample before shipping improves the preservation of cell morphology up to 96 hours after collection.https://doi.org/10.1111/jvim.16637autologous serumcerebroCSFhorsespinal fluidspinal fluid |
| spellingShingle | Camilla Quattrini Rebeca Scalco William Vernau Pouya Dini Monica Aleman Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses Journal of Veterinary Internal Medicine autologous serum cerebro CSF horse spinal fluid spinal fluid |
| title | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| title_full | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| title_fullStr | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| title_full_unstemmed | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| title_short | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| title_sort | effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
| topic | autologous serum cerebro CSF horse spinal fluid spinal fluid |
| url | https://doi.org/10.1111/jvim.16637 |
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