No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease
Abstract Prominent features of HD neuropathology are the intranuclear and cytoplasmic inclusions of huntingtin and striatal and cortical neuronal cell death. Recently, synaptic defects have been reported on HD-related studies, including impairment of neurotransmitter release and alterations of synap...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Acta Neuropathologica Communications |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40478-020-00949-y |
| _version_ | 1818516481786249216 |
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| author | Ting-Ting Huang Ruben Smith Karl Bacos Dong-Yan Song Richard M. Faull Henry J. Waldvogel Jia-Yi Li |
| author_facet | Ting-Ting Huang Ruben Smith Karl Bacos Dong-Yan Song Richard M. Faull Henry J. Waldvogel Jia-Yi Li |
| author_sort | Ting-Ting Huang |
| collection | DOAJ |
| description | Abstract Prominent features of HD neuropathology are the intranuclear and cytoplasmic inclusions of huntingtin and striatal and cortical neuronal cell death. Recently, synaptic defects have been reported on HD-related studies, including impairment of neurotransmitter release and alterations of synaptic components. However, the definite characteristics of synapse dysfunction and the underlying mechanisms remain largely unknown. We studied the gene expression levels and patterns of a number of proteins forming the cytoskeletal matrix of the presynaptic active zones in HD transgenic mice (R6/1), in hippocampal neuronal cultures overexpressing mutant huntingtin and in postmortem brain tissues of HD patients. To investigate the interactions between huntingtin and active proteins, we performed confocal microscopic imaging and immunoprecipitation in mouse and HEK 293 cell line models. The mRNA and protein levels of Bassoon were reduced in mouse and cell culture models of HD and in brain tissues of patients with HD. Moreover, a striking re-distribution of a complex of proteins including Bassoon, Piccolo and Munc 13–1 from the cytoplasm and synapses into intranuclear huntingtin aggregates with loss of active zone proteins and dendritic spines. This re-localization was age-dependent and coincided with the formation of huntingtin aggregates. Using co-immunoprecipitation, we demonstrated that huntingtin interacts with Bassoon, and that this interaction is likely mediated by a third linking protein. Three structural proteins involved in neurotransmitter release in the presynaptic active zones of neurons are altered in expression and that the proteins are redistributed from their normal functional site into mutant huntingtin aggregates. |
| first_indexed | 2024-12-11T00:42:51Z |
| format | Article |
| id | doaj.art-24f25994bcd54b5da2d577ede39c731c |
| institution | Directory Open Access Journal |
| issn | 2051-5960 |
| language | English |
| last_indexed | 2024-12-11T00:42:51Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Neuropathologica Communications |
| spelling | doaj.art-24f25994bcd54b5da2d577ede39c731c2022-12-22T01:26:51ZengBMCActa Neuropathologica Communications2051-59602020-06-018111610.1186/s40478-020-00949-yNo symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s diseaseTing-Ting Huang0Ruben Smith1Karl Bacos2Dong-Yan Song3Richard M. Faull4Henry J. Waldvogel5Jia-Yi Li6Institute of Neuroscience, College of Life and Health Sciences, Northeastern UniversityDepartment of Neurology, Skåne University Hospital, Lund UniversityEpigenetics and Diabetes Unit, Department of Clinical Sciences, Lund University Diabetes CentreInstitute of Neuroscience, College of Life and Health Sciences, Northeastern UniversityCentre for Brain Research and Department of Anatomy and Medical Imaging, Faculty of Medical and Health Sciences, The University of AucklandCentre for Brain Research and Department of Anatomy and Medical Imaging, Faculty of Medical and Health Sciences, The University of AucklandInstitute of Neuroscience, College of Life and Health Sciences, Northeastern UniversityAbstract Prominent features of HD neuropathology are the intranuclear and cytoplasmic inclusions of huntingtin and striatal and cortical neuronal cell death. Recently, synaptic defects have been reported on HD-related studies, including impairment of neurotransmitter release and alterations of synaptic components. However, the definite characteristics of synapse dysfunction and the underlying mechanisms remain largely unknown. We studied the gene expression levels and patterns of a number of proteins forming the cytoskeletal matrix of the presynaptic active zones in HD transgenic mice (R6/1), in hippocampal neuronal cultures overexpressing mutant huntingtin and in postmortem brain tissues of HD patients. To investigate the interactions between huntingtin and active proteins, we performed confocal microscopic imaging and immunoprecipitation in mouse and HEK 293 cell line models. The mRNA and protein levels of Bassoon were reduced in mouse and cell culture models of HD and in brain tissues of patients with HD. Moreover, a striking re-distribution of a complex of proteins including Bassoon, Piccolo and Munc 13–1 from the cytoplasm and synapses into intranuclear huntingtin aggregates with loss of active zone proteins and dendritic spines. This re-localization was age-dependent and coincided with the formation of huntingtin aggregates. Using co-immunoprecipitation, we demonstrated that huntingtin interacts with Bassoon, and that this interaction is likely mediated by a third linking protein. Three structural proteins involved in neurotransmitter release in the presynaptic active zones of neurons are altered in expression and that the proteins are redistributed from their normal functional site into mutant huntingtin aggregates.http://link.springer.com/article/10.1186/s40478-020-00949-yHuntington’s diseaseActive zone proteinsSynaptic dysfunctionProtein aggregation |
| spellingShingle | Ting-Ting Huang Ruben Smith Karl Bacos Dong-Yan Song Richard M. Faull Henry J. Waldvogel Jia-Yi Li No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease Acta Neuropathologica Communications Huntington’s disease Active zone proteins Synaptic dysfunction Protein aggregation |
| title | No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease |
| title_full | No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease |
| title_fullStr | No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease |
| title_full_unstemmed | No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease |
| title_short | No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington’s disease |
| title_sort | no symphony without bassoon and piccolo changes in synaptic active zone proteins in huntington s disease |
| topic | Huntington’s disease Active zone proteins Synaptic dysfunction Protein aggregation |
| url | http://link.springer.com/article/10.1186/s40478-020-00949-y |
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