Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel
Charcoal rot (CR) disease caused by Macrophomina phaseolina is responsible for significant yield losses in soybean production. Among the methods available for controlling this disease, breeding for resistance is the most promising. Progress in breeding efforts has been slow due to the insufficient i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01626/full |
| _version_ | 1819030788616749056 |
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| author | Sara M. Coser R. V. Chowda Reddy Jiaoping Zhang Daren S. Mueller Alemu Mengistu Kiersten A. Wise Tom W. Allen Arti Singh Asheesh K. Singh |
| author_facet | Sara M. Coser R. V. Chowda Reddy Jiaoping Zhang Daren S. Mueller Alemu Mengistu Kiersten A. Wise Tom W. Allen Arti Singh Asheesh K. Singh |
| author_sort | Sara M. Coser |
| collection | DOAJ |
| description | Charcoal rot (CR) disease caused by Macrophomina phaseolina is responsible for significant yield losses in soybean production. Among the methods available for controlling this disease, breeding for resistance is the most promising. Progress in breeding efforts has been slow due to the insufficient information available on the genetic mechanisms related to resistance. Genome-wide association studies (GWAS) enable unraveling the genetic architecture of resistance and identification of causal genes. The aims of this study were to identify new sources of resistance to CR in a collection of 459 diverse plant introductions from the USDA Soybean Germplasm Core Collection using field and greenhouse screenings, and to conduct GWAS to identify candidate genes and associated molecular markers. New sources for CR resistance were identified from both field and greenhouse screening from maturity groups I, II, and III. Five significant single nucleotide polymorphism (SNP) and putative candidate genes related to abiotic and biotic stress responses are reported from the field screening; while greenhouse screening revealed eight loci associated with eight candidate gene families, all associated with functions controlling plant defense response. No overlap of markers or genes was observed between field and greenhouse screenings suggesting a complex molecular mechanism underlying resistance to CR in soybean with varied response to different environments; but our findings provide useful information for advancing breeding for CR resistance as well as the genetic mechanism of resistance. |
| first_indexed | 2024-12-21T06:35:43Z |
| format | Article |
| id | doaj.art-24f29d56371a4665b3c5cec74b13554c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T06:35:43Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-24f29d56371a4665b3c5cec74b13554c2022-12-21T19:12:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-09-01810.3389/fpls.2017.01626278088Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse PanelSara M. Coser0R. V. Chowda Reddy1Jiaoping Zhang2Daren S. Mueller3Alemu Mengistu4Kiersten A. Wise5Tom W. Allen6Arti Singh7Asheesh K. Singh8Department of Agronomy, Iowa State UniversityAmes, IA, United StatesDepartment of Agronomy, Iowa State UniversityAmes, IA, United StatesDepartment of Agronomy, Iowa State UniversityAmes, IA, United StatesDepartment of Plant Pathology and Microbiology, Iowa State UniversityAmes, IA, United StatesCrop Genetics Research Unit, United States Department of Agriculture, Agricultural Research ServiceJackson, TN, United StatesDepartment of Botany and Plant Pathology, Purdue UniversityWest Lafayette, IN, United StatesDelta Research and Extension Center, Mississippi State UniversityStoneville, MS, United StatesDepartment of Agronomy, Iowa State UniversityAmes, IA, United StatesDepartment of Agronomy, Iowa State UniversityAmes, IA, United StatesCharcoal rot (CR) disease caused by Macrophomina phaseolina is responsible for significant yield losses in soybean production. Among the methods available for controlling this disease, breeding for resistance is the most promising. Progress in breeding efforts has been slow due to the insufficient information available on the genetic mechanisms related to resistance. Genome-wide association studies (GWAS) enable unraveling the genetic architecture of resistance and identification of causal genes. The aims of this study were to identify new sources of resistance to CR in a collection of 459 diverse plant introductions from the USDA Soybean Germplasm Core Collection using field and greenhouse screenings, and to conduct GWAS to identify candidate genes and associated molecular markers. New sources for CR resistance were identified from both field and greenhouse screening from maturity groups I, II, and III. Five significant single nucleotide polymorphism (SNP) and putative candidate genes related to abiotic and biotic stress responses are reported from the field screening; while greenhouse screening revealed eight loci associated with eight candidate gene families, all associated with functions controlling plant defense response. No overlap of markers or genes was observed between field and greenhouse screenings suggesting a complex molecular mechanism underlying resistance to CR in soybean with varied response to different environments; but our findings provide useful information for advancing breeding for CR resistance as well as the genetic mechanism of resistance.http://journal.frontiersin.org/article/10.3389/fpls.2017.01626/fullGWASMacrophomina phaseolinasoybeanbreedingresistancecharcoal rot |
| spellingShingle | Sara M. Coser R. V. Chowda Reddy Jiaoping Zhang Daren S. Mueller Alemu Mengistu Kiersten A. Wise Tom W. Allen Arti Singh Asheesh K. Singh Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel Frontiers in Plant Science GWAS Macrophomina phaseolina soybean breeding resistance charcoal rot |
| title | Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel |
| title_full | Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel |
| title_fullStr | Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel |
| title_full_unstemmed | Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel |
| title_short | Genetic Architecture of Charcoal Rot (Macrophomina phaseolina) Resistance in Soybean Revealed Using a Diverse Panel |
| title_sort | genetic architecture of charcoal rot macrophomina phaseolina resistance in soybean revealed using a diverse panel |
| topic | GWAS Macrophomina phaseolina soybean breeding resistance charcoal rot |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01626/full |
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