Antimicrobial Activity and Stability of Electron Beam Irradiated Dental Irrigants
Background: The electron beam (e-beam) radiation is considered as an effective means of sterilization of healthcare products as well as to induce the structural changes in the pharmaceutical agents/drug molecules. In addition to structural changes of pharmaceutical it also induces the formation...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5180/9449_CE(Ra)_F(Sh)_PF1(SNAK)_PFA(Sh).pdf |
| Summary: | Background: The electron beam (e-beam) radiation is
considered as an effective means of sterilization of healthcare
products as well as to induce the structural changes in the
pharmaceutical agents/drug molecules. In addition to structural
changes of pharmaceutical it also induces the formation of
low molecular weight compounds with altered microbiological,
physicochemical and toxicological properties. Among the
several known medicaments, sodium hypochlorite (NaOCl) and
chlorhexidine digluconate (CHX) are used as irrigants in dentistry
to kill the pathogenic microorganisms like Enterococcus faecalis,
Staphylococcus aureus, Streptococcus mutans and Candida
albicans inhabiting the oral cavity.
Objectives: The aim of this study was to evaluate the
antimicrobial activity and stability of e-beam irradiated dental
irrigants, NaOCl and CHX.
Materials and Methods: Two dental irrigants NaOCl (1.25% and
2.5%) and CHX (1% and 2%) were exposed to various doses
of e-beam radiation. The antimicrobial activities of e-beam
irradiated irrigants were compared with the non-irradiated
(control) irrigants against E. faecalis, S. aureus, S. mutans and
C. albicans by disc diffusion method. Following the storage,
physico-chemical properties of the irrigants were recorded and
the cytotoxic effect was evaluated on human gingival fibroblast
cells.
Result: The irrigants, 1.25% NaOCl and 1% CHX showed
significantly increased antimicrobial activity against both E.
faecalis, (16+0.0) and S. aureus (25+0.0) after irradiation with
1 kGy e-beam. Whereas, 2.5% NaOCl and 2% CHX showed
slightly increased antimicrobial activity only against S.
aureus (28+0.0). The significant difference was noticed in the
antimicrobial activity and cytotoxicity of irradiated and nonirradiated irrigants following the storage for 180 d at 40
C.
Conclusion: The e-beam irradiation increased the antimicrobial
activity of irrigants without altering the biocompatibility. |
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| ISSN: | 2249-782X 0973-709X |