EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone
Current pharmacological therapies against demyelinating diseases are not quite satisfactory to promote remyelination. Epidermal growth factor (EGF) can expand the population of oligodendrocyte precursor cells (OPCs) that may help with the remyelination process, but its delivery into the injured tiss...
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MDPI AG
2022-02-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/3/333 |
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| author | Eduardo Lira-Diaz Jesus Monroy-Rodriguez Maria G. Gonzalez-Pedroza Raul A. Morales-Luckie Luis Castro-Sánchez Oscar Gonzalez-Perez |
| author_facet | Eduardo Lira-Diaz Jesus Monroy-Rodriguez Maria G. Gonzalez-Pedroza Raul A. Morales-Luckie Luis Castro-Sánchez Oscar Gonzalez-Perez |
| author_sort | Eduardo Lira-Diaz |
| collection | DOAJ |
| description | Current pharmacological therapies against demyelinating diseases are not quite satisfactory to promote remyelination. Epidermal growth factor (EGF) can expand the population of oligodendrocyte precursor cells (OPCs) that may help with the remyelination process, but its delivery into the injured tissue is still a biomedical challenge. Gold nanoparticles (GNPs) may be a useful tool for drug delivery into the brain. To evaluate remyelination in the septal nucleus, we administered intracerebral GNPs coupled with EGF (EGF–GNPs). C57BL6/J mice were demyelinated with 0.4% cuprizone (CPZ) and divided into several groups: Sham, Ctrl, GNPs, EGF, and EGF–GNPs. We evaluated the remyelination process at two time-points: 2 weeks and 3 weeks post-injection (WPI) of each treatment. We used the rotarod for evaluating motor coordination. Then, we did a Western blot analysis myelin-associated proteins: CNPase, MAG, MOG, and MBP. EGF–GNPs increase the expression of CNPase, MAG, and MOG at 2 WPI. At 3 WPI, we found that the EGF–GNPs treatment improves motor coordination and increases MAG, MOG, and MBP. EGF–GNPs enhance the expression of myelin-associated proteins and improve the motor coordination in mice. Thus, EGF-associated GNPs may be a promising pharmacological vehicle for delivering long-lasting drugs into the brain. |
| first_indexed | 2024-03-09T13:34:49Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T13:34:49Z |
| publishDate | 2022-02-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-24f73c4b32514accbe586841328af9fb2023-11-30T21:13:14ZengMDPI AGLife2075-17292022-02-0112333310.3390/life12030333EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by CuprizoneEduardo Lira-Diaz0Jesus Monroy-Rodriguez1Maria G. Gonzalez-Pedroza2Raul A. Morales-Luckie3Luis Castro-Sánchez4Oscar Gonzalez-Perez5Laboratory of Neuroscience, School of Psychology, University of Colima, Colima 28040, MexicoUniversity Center for Biomedical Research, University of Colima, Colima 28045, MexicoDepartment of Biotechnology, School of Sciences, Autonomous University of the State of Mexico, Toluca 50200, MexicoDepartment of Nanomaterials, Joint Center for Research in Sustainable Chemistry UAEMex—UNAM (CCIQS), Autonomous University of the State of Mexico (UAEMex), Toluca 50200, MexicoUniversity Center for Biomedical Research, University of Colima, Colima 28045, MexicoLaboratory of Neuroscience, School of Psychology, University of Colima, Colima 28040, MexicoCurrent pharmacological therapies against demyelinating diseases are not quite satisfactory to promote remyelination. Epidermal growth factor (EGF) can expand the population of oligodendrocyte precursor cells (OPCs) that may help with the remyelination process, but its delivery into the injured tissue is still a biomedical challenge. Gold nanoparticles (GNPs) may be a useful tool for drug delivery into the brain. To evaluate remyelination in the septal nucleus, we administered intracerebral GNPs coupled with EGF (EGF–GNPs). C57BL6/J mice were demyelinated with 0.4% cuprizone (CPZ) and divided into several groups: Sham, Ctrl, GNPs, EGF, and EGF–GNPs. We evaluated the remyelination process at two time-points: 2 weeks and 3 weeks post-injection (WPI) of each treatment. We used the rotarod for evaluating motor coordination. Then, we did a Western blot analysis myelin-associated proteins: CNPase, MAG, MOG, and MBP. EGF–GNPs increase the expression of CNPase, MAG, and MOG at 2 WPI. At 3 WPI, we found that the EGF–GNPs treatment improves motor coordination and increases MAG, MOG, and MBP. EGF–GNPs enhance the expression of myelin-associated proteins and improve the motor coordination in mice. Thus, EGF-associated GNPs may be a promising pharmacological vehicle for delivering long-lasting drugs into the brain.https://www.mdpi.com/2075-1729/12/3/333epidermal growth factorremyelinationgold nanoparticlesCNPaseMAGMOG |
| spellingShingle | Eduardo Lira-Diaz Jesus Monroy-Rodriguez Maria G. Gonzalez-Pedroza Raul A. Morales-Luckie Luis Castro-Sánchez Oscar Gonzalez-Perez EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone Life epidermal growth factor remyelination gold nanoparticles CNPase MAG MOG |
| title | EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone |
| title_full | EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone |
| title_fullStr | EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone |
| title_full_unstemmed | EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone |
| title_short | EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone |
| title_sort | egf coupled gold nanoparticles increase the expression of cnpase and the myelin associated proteins mag mog and mbp in the septal nucleus demyelinated by cuprizone |
| topic | epidermal growth factor remyelination gold nanoparticles CNPase MAG MOG |
| url | https://www.mdpi.com/2075-1729/12/3/333 |
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