An Improved Animal Model of Multiple Myeloma Bone Disease
Multiple myeloma (MM) is a plasma cell malignancy that causes an accumulation of terminally differentiated monoclonal plasma cells in the bone marrow, accompanied by multiple myeloma bone disease (MMBD). MM animal models have been developed and enable to interrogate the mechanism of MM tumorigenesis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/17/4277 |
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| author | Syed Hassan Mehdi Carol A Morris Jung Ae Lee Donghoon Yoon |
| author_facet | Syed Hassan Mehdi Carol A Morris Jung Ae Lee Donghoon Yoon |
| author_sort | Syed Hassan Mehdi |
| collection | DOAJ |
| description | Multiple myeloma (MM) is a plasma cell malignancy that causes an accumulation of terminally differentiated monoclonal plasma cells in the bone marrow, accompanied by multiple myeloma bone disease (MMBD). MM animal models have been developed and enable to interrogate the mechanism of MM tumorigenesis. However, these models demonstrate little or no evidence of MMBD. We try to establish the MMBD model with severe bone lesions and easily accessible MM progression. 1 × 10<sup>6</sup> luciferase-expressing 5TGM1 cells were injected into 8–12 week-old NOD SCID gamma mouse (NSG) and C57BL/KaLwRij mouse via the tail vein. Myeloma progression was assessed weekly via in vivo bioluminescence (BL) imaging using IVIS-200. The spine and femur/tibia were extracted and scanned by the micro-computer tomography for bone histo-morphometric analyses at the postmortem. The median survivals were 56 days in NSG while 44.5 days in C57BL/KaLwRij agreed with the BL imaging results. Histomorphic and DEXA analyses demonstrated that NSG mice have severe bone resorption that occurred at the lumbar spine but no significance at the femur compared to C57BL/KaLwRij mice. Based on these, we conclude that the systemic 5TGM1 injected NSG mouse slowly progresses myeloma and develops more severe MMBD than the C57BL/KaLwRij model. |
| first_indexed | 2024-03-10T08:14:21Z |
| format | Article |
| id | doaj.art-24f7bec2b74e4803b06d42262f9cfa30 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T08:14:21Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-24f7bec2b74e4803b06d42262f9cfa302023-11-22T10:24:55ZengMDPI AGCancers2072-66942021-08-011317427710.3390/cancers13174277An Improved Animal Model of Multiple Myeloma Bone DiseaseSyed Hassan Mehdi0Carol A Morris1Jung Ae Lee2Donghoon Yoon3Myeloma Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAGraduate Program in Interdisciplinary Biomedical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA 01605, USAMyeloma Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAMultiple myeloma (MM) is a plasma cell malignancy that causes an accumulation of terminally differentiated monoclonal plasma cells in the bone marrow, accompanied by multiple myeloma bone disease (MMBD). MM animal models have been developed and enable to interrogate the mechanism of MM tumorigenesis. However, these models demonstrate little or no evidence of MMBD. We try to establish the MMBD model with severe bone lesions and easily accessible MM progression. 1 × 10<sup>6</sup> luciferase-expressing 5TGM1 cells were injected into 8–12 week-old NOD SCID gamma mouse (NSG) and C57BL/KaLwRij mouse via the tail vein. Myeloma progression was assessed weekly via in vivo bioluminescence (BL) imaging using IVIS-200. The spine and femur/tibia were extracted and scanned by the micro-computer tomography for bone histo-morphometric analyses at the postmortem. The median survivals were 56 days in NSG while 44.5 days in C57BL/KaLwRij agreed with the BL imaging results. Histomorphic and DEXA analyses demonstrated that NSG mice have severe bone resorption that occurred at the lumbar spine but no significance at the femur compared to C57BL/KaLwRij mice. Based on these, we conclude that the systemic 5TGM1 injected NSG mouse slowly progresses myeloma and develops more severe MMBD than the C57BL/KaLwRij model.https://www.mdpi.com/2072-6694/13/17/4277murine modelmultiple myeloma bone diseasesevere osteolytic lesions |
| spellingShingle | Syed Hassan Mehdi Carol A Morris Jung Ae Lee Donghoon Yoon An Improved Animal Model of Multiple Myeloma Bone Disease Cancers murine model multiple myeloma bone disease severe osteolytic lesions |
| title | An Improved Animal Model of Multiple Myeloma Bone Disease |
| title_full | An Improved Animal Model of Multiple Myeloma Bone Disease |
| title_fullStr | An Improved Animal Model of Multiple Myeloma Bone Disease |
| title_full_unstemmed | An Improved Animal Model of Multiple Myeloma Bone Disease |
| title_short | An Improved Animal Model of Multiple Myeloma Bone Disease |
| title_sort | improved animal model of multiple myeloma bone disease |
| topic | murine model multiple myeloma bone disease severe osteolytic lesions |
| url | https://www.mdpi.com/2072-6694/13/17/4277 |
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