The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses
Sisal is a common name for different plant varieties in the genus <i>Agave</i> (especially <i>Agave sisalana</i>) used for high-quality natural leaf fiber extraction. Despite the economic value of these plants, we still lack information about the diversity of viruses (virome)...
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1704 |
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| author | Gabriel Quintanilha-Peixoto Paula Luize Camargos Fonseca Fábio Trigo Raya Marina Pupke Marone Dener Eduardo Bortolini Piotr Mieczkowski Roenick Proveti Olmo Marcelo Falsarella Carazzolle Christian A. Voigt Ana Cristina Fermino Soares Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto Eric Roberto Guimarães Rocha Aguiar |
| author_facet | Gabriel Quintanilha-Peixoto Paula Luize Camargos Fonseca Fábio Trigo Raya Marina Pupke Marone Dener Eduardo Bortolini Piotr Mieczkowski Roenick Proveti Olmo Marcelo Falsarella Carazzolle Christian A. Voigt Ana Cristina Fermino Soares Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto Eric Roberto Guimarães Rocha Aguiar |
| author_sort | Gabriel Quintanilha-Peixoto |
| collection | DOAJ |
| description | Sisal is a common name for different plant varieties in the genus <i>Agave</i> (especially <i>Agave sisalana</i>) used for high-quality natural leaf fiber extraction. Despite the economic value of these plants, we still lack information about the diversity of viruses (virome) in non-<i>tequilana</i> species from the genus <i>Agave</i>. In this work, by associating RNA and DNA deep sequencing we were able to identify 25 putative viral species infecting <i>A. sisalana, A. fourcroydes,</i> and <i>Agave</i> hybrid 11648, including one strain of <i>Cowpea Mild Mottle Virus</i> (CPMMV) and 24 elements likely representing new viruses. Phylogenetic analysis indicated they belong to at least six viral families: Alphaflexiviridae, Betaflexiviridae, Botourmiaviridae, Closteroviridae, Partitiviridae, Virgaviridae, and three distinct unclassified groups. We observed higher viral taxa richness in roots when compared to leaves and stems. Furthermore, leaves and stems are very similar diversity-wise, with a lower number of taxa and dominance of a single viral species. Finally, approximately 50% of the identified viruses were found in all Agave organs investigated, which suggests that they likely produce a systemic infection. This is the first metatranscriptomics study focused on viral identification in species from the genus <i>Agave</i>. Despite having analyzed symptomless individuals, we identified several viruses supposedly infecting <i>Agave</i> species, including organ-specific and systemic species. Surprisingly, some of these putative viruses are probably infecting microorganisms composing the plant microbiota. Altogether, our results reinforce the importance of unbiased strategies for the identification and monitoring of viruses in plant species, including those with asymptomatic phenotypes. |
| first_indexed | 2024-03-10T08:33:54Z |
| format | Article |
| id | doaj.art-24f9dd087e6a459f8747edf97f48bde9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:33:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-24f9dd087e6a459f8747edf97f48bde92023-11-22T08:47:37ZengMDPI AGMicroorganisms2076-26072021-08-0198170410.3390/microorganisms9081704The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific VirusesGabriel Quintanilha-Peixoto0Paula Luize Camargos Fonseca1Fábio Trigo Raya2Marina Pupke Marone3Dener Eduardo Bortolini4Piotr Mieczkowski5Roenick Proveti Olmo6Marcelo Falsarella Carazzolle7Christian A. Voigt8Ana Cristina Fermino Soares9Gonçalo Amarante Guimarães Pereira10Aristóteles Góes-Neto11Eric Roberto Guimarães Rocha Aguiar12Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilInstitute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilDepartment of Genetics and Evolution, Institute of Biology, Universidade Estadual de Campinas, Campinas 13083-872, BrazilDepartment of Genetics and Evolution, Institute of Biology, Universidade Estadual de Campinas, Campinas 13083-872, BrazilInstitute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilHigh-Throughput Sequencing Facility, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27516, USAInstitute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilDepartment of Genetics and Evolution, Institute of Biology, Universidade Estadual de Campinas, Campinas 13083-872, BrazilBASF Innovation Center Gent, 9052 Gent, BelgiumCenter of Agricultural, Environmental and Biological Sciences, Universidade Federal do Recôncavo da Bahia, Cruz das Almas 44380-000, BrazilDepartment of Genetics and Evolution, Institute of Biology, Universidade Estadual de Campinas, Campinas 13083-872, BrazilInstitute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, BrazilCenter of Biotechnology and Genetics, Department of Biological Science, Universidade Estadual de Santa Cruz, Ilhéus 45662-900, BrazilSisal is a common name for different plant varieties in the genus <i>Agave</i> (especially <i>Agave sisalana</i>) used for high-quality natural leaf fiber extraction. Despite the economic value of these plants, we still lack information about the diversity of viruses (virome) in non-<i>tequilana</i> species from the genus <i>Agave</i>. In this work, by associating RNA and DNA deep sequencing we were able to identify 25 putative viral species infecting <i>A. sisalana, A. fourcroydes,</i> and <i>Agave</i> hybrid 11648, including one strain of <i>Cowpea Mild Mottle Virus</i> (CPMMV) and 24 elements likely representing new viruses. Phylogenetic analysis indicated they belong to at least six viral families: Alphaflexiviridae, Betaflexiviridae, Botourmiaviridae, Closteroviridae, Partitiviridae, Virgaviridae, and three distinct unclassified groups. We observed higher viral taxa richness in roots when compared to leaves and stems. Furthermore, leaves and stems are very similar diversity-wise, with a lower number of taxa and dominance of a single viral species. Finally, approximately 50% of the identified viruses were found in all Agave organs investigated, which suggests that they likely produce a systemic infection. This is the first metatranscriptomics study focused on viral identification in species from the genus <i>Agave</i>. Despite having analyzed symptomless individuals, we identified several viruses supposedly infecting <i>Agave</i> species, including organ-specific and systemic species. Surprisingly, some of these putative viruses are probably infecting microorganisms composing the plant microbiota. Altogether, our results reinforce the importance of unbiased strategies for the identification and monitoring of viruses in plant species, including those with asymptomatic phenotypes.https://www.mdpi.com/2076-2607/9/8/1704viromemetatranscriptomics<i>Agave</i> |
| spellingShingle | Gabriel Quintanilha-Peixoto Paula Luize Camargos Fonseca Fábio Trigo Raya Marina Pupke Marone Dener Eduardo Bortolini Piotr Mieczkowski Roenick Proveti Olmo Marcelo Falsarella Carazzolle Christian A. Voigt Ana Cristina Fermino Soares Gonçalo Amarante Guimarães Pereira Aristóteles Góes-Neto Eric Roberto Guimarães Rocha Aguiar The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses Microorganisms virome metatranscriptomics <i>Agave</i> |
| title | The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses |
| title_full | The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses |
| title_fullStr | The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses |
| title_full_unstemmed | The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses |
| title_short | The Sisal Virome: Uncovering the Viral Diversity of <i>Agave</i> Varieties Reveals New and Organ-Specific Viruses |
| title_sort | sisal virome uncovering the viral diversity of i agave i varieties reveals new and organ specific viruses |
| topic | virome metatranscriptomics <i>Agave</i> |
| url | https://www.mdpi.com/2076-2607/9/8/1704 |
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