Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines
Universal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/3/269 |
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| author | David Salcines-Cuevas Hector Terán-Navarro Ricardo Calderón-Gonzalez Paula Torres-Rodriguez Raquel Tobes Manuel Fresno Jorge Calvo-Montes I. Concepción Pérez Del Molino-Bernal Sonsoles Yañez-Diaz Carmen Alvarez-Dominguez |
| author_facet | David Salcines-Cuevas Hector Terán-Navarro Ricardo Calderón-Gonzalez Paula Torres-Rodriguez Raquel Tobes Manuel Fresno Jorge Calvo-Montes I. Concepción Pérez Del Molino-Bernal Sonsoles Yañez-Diaz Carmen Alvarez-Dominguez |
| author_sort | David Salcines-Cuevas |
| collection | DOAJ |
| description | Universal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides, GAPDH-L1 (<i>Listeria</i>), GAPDH-M1 (<i>Mycobacterium</i>) and GAPDH-S1 (<i>Streptococcus</i>), share 95–98.55% sequence homology, biochemical and MHC binding abilities and, therefore, are good candidates for universal vaccine designs. Here, we used dendritic cells (DC) as vaccine platforms to test GAPDH epitopes that conferred protection against <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> or <i>Streptococcus pneumoniae</i> in our search of epitopes for universal vaccines. DC loaded with GAPDH-L1, GAPDH-M1 or GAPDH-S1 peptides show high immunogenicity measured by the cellular DTH responses in mice, lacked toxicity and were capable of cross-protection immunity against mice infections with each one of the pathogens. Vaccine efficiency correlated with high titers of anti-GAPDH-L1 antibodies in sera of vaccinated mice, a Th1 cytokine pattern and high frequencies of GAPDH-L1-specific CD4+ and CD8+ T cells and IFN-γ producers in the spleens. We concluded that GAPDH-L1 peptide was the best epitope for universal vaccines in the <i>Listeria</i>, <i>Mycobacterium</i> or <i>Streptococcus</i> taxonomic groups, whose pathogenic strains caused relevant morbidities in adults and especially in the elderly. |
| first_indexed | 2024-03-10T13:08:28Z |
| format | Article |
| id | doaj.art-24fa41b5074440188a98730e020226d7 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T13:08:28Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-24fa41b5074440188a98730e020226d72023-11-21T10:54:22ZengMDPI AGVaccines2076-393X2021-03-019326910.3390/vaccines9030269Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal VaccinesDavid Salcines-Cuevas0Hector Terán-Navarro1Ricardo Calderón-Gonzalez2Paula Torres-Rodriguez3Raquel Tobes4Manuel Fresno5Jorge Calvo-Montes6I. Concepción Pérez Del Molino-Bernal7Sonsoles Yañez-Diaz8Carmen Alvarez-Dominguez9Grupo de Oncología y Nanovacunas, Instituto de Investigación Marqués de Valdecilla, 39011 Santander, Cantabria, SpainGrupo de Oncología y Nanovacunas, Instituto de Investigación Marqués de Valdecilla, 39011 Santander, Cantabria, SpainGrupo de Oncología y Nanovacunas, Instituto de Investigación Marqués de Valdecilla, 39011 Santander, Cantabria, SpainGrupo de Oncología y Nanovacunas, Instituto de Investigación Marqués de Valdecilla, 39011 Santander, Cantabria, SpainAlamo Blanco, 18110 Granada, Andalucia, SpainCentro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid & DIOMUNE S.L., Parque Científico de Madrid, 28049 Madrid, SpainServicio de Microbiología, Hospital Universitario Marqués de Valdecilla, 39008 Santander, Cantabria, SpainServicio de Microbiología, Hospital Universitario Marqués de Valdecilla, 39008 Santander, Cantabria, SpainServicio de Dermatología, Hospital Universitario Marqués de Valdecilla, 39008 Santander, Cantabria, SpainFacultad de Educación y Facultad de Ciencias de la Salud, Universidad Internacional de La Rioja, Avda. La Paz 137, 26006 Logroño, La Rioja, SpainUniversal vaccines can be prepared with antigens common to different pathogens. In this regard, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a common virulence factor among pathogenic bacteria of the genera Listeria, Mycobacterium and Streptococcus. Their N-terminal 22 amino acid peptides, GAPDH-L1 (<i>Listeria</i>), GAPDH-M1 (<i>Mycobacterium</i>) and GAPDH-S1 (<i>Streptococcus</i>), share 95–98.55% sequence homology, biochemical and MHC binding abilities and, therefore, are good candidates for universal vaccine designs. Here, we used dendritic cells (DC) as vaccine platforms to test GAPDH epitopes that conferred protection against <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> or <i>Streptococcus pneumoniae</i> in our search of epitopes for universal vaccines. DC loaded with GAPDH-L1, GAPDH-M1 or GAPDH-S1 peptides show high immunogenicity measured by the cellular DTH responses in mice, lacked toxicity and were capable of cross-protection immunity against mice infections with each one of the pathogens. Vaccine efficiency correlated with high titers of anti-GAPDH-L1 antibodies in sera of vaccinated mice, a Th1 cytokine pattern and high frequencies of GAPDH-L1-specific CD4+ and CD8+ T cells and IFN-γ producers in the spleens. We concluded that GAPDH-L1 peptide was the best epitope for universal vaccines in the <i>Listeria</i>, <i>Mycobacterium</i> or <i>Streptococcus</i> taxonomic groups, whose pathogenic strains caused relevant morbidities in adults and especially in the elderly.https://www.mdpi.com/2076-393X/9/3/269adjuvantsglyceraldehyde-3-phosphate-dehydrogenaselisteriosispneumoniatuberculosisvaccines |
| spellingShingle | David Salcines-Cuevas Hector Terán-Navarro Ricardo Calderón-Gonzalez Paula Torres-Rodriguez Raquel Tobes Manuel Fresno Jorge Calvo-Montes I. Concepción Pérez Del Molino-Bernal Sonsoles Yañez-Diaz Carmen Alvarez-Dominguez Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines Vaccines adjuvants glyceraldehyde-3-phosphate-dehydrogenase listeriosis pneumonia tuberculosis vaccines |
| title | Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines |
| title_full | Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines |
| title_fullStr | Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines |
| title_full_unstemmed | Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines |
| title_short | Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of <i>Listeria monocytogenes</i>, <i>Mycobacterium marinum</i> and <i>Streptococcus pneumoniae</i> as Universal Vaccines |
| title_sort | glyceraldehyde 3 phosphate dehydrogenase common peptides of i listeria monocytogenes i i mycobacterium marinum i and i streptococcus pneumoniae i as universal vaccines |
| topic | adjuvants glyceraldehyde-3-phosphate-dehydrogenase listeriosis pneumonia tuberculosis vaccines |
| url | https://www.mdpi.com/2076-393X/9/3/269 |
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