From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry a...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/3741 |
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| author | Ilaria Sorrentino Ilaria Stanzione Yannig Nedellec Alessandra Piscitelli Paola Giardina Alan Le Goff |
| author_facet | Ilaria Sorrentino Ilaria Stanzione Yannig Nedellec Alessandra Piscitelli Paola Giardina Alan Le Goff |
| author_sort | Ilaria Sorrentino |
| collection | DOAJ |
| description | A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications. |
| first_indexed | 2024-03-10T19:35:25Z |
| format | Article |
| id | doaj.art-24fadafe05714f228de83c32f50b376f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:35:25Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-24fadafe05714f228de83c32f50b376f2023-11-20T01:44:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012111374110.3390/ijms21113741From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric EnzymeIlaria Sorrentino0Ilaria Stanzione1Yannig Nedellec2Alessandra Piscitelli3Paola Giardina4Alan Le Goff5Department of Molecular Chemistry, University Grenoble Alpes, CNRS, 38000 Grenoble, FranceDepartment of Chemical Sciences, University Federico II, 80126 Naples, ItalyDepartment of Molecular Chemistry, University Grenoble Alpes, CNRS, 38000 Grenoble, FranceDepartment of Chemical Sciences, University Federico II, 80126 Naples, ItalyDepartment of Chemical Sciences, University Federico II, 80126 Naples, ItalyDepartment of Molecular Chemistry, University Grenoble Alpes, CNRS, 38000 Grenoble, FranceA chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications.https://www.mdpi.com/1422-0067/21/11/3741laccasehydrophobingraphene |
| spellingShingle | Ilaria Sorrentino Ilaria Stanzione Yannig Nedellec Alessandra Piscitelli Paola Giardina Alan Le Goff From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme International Journal of Molecular Sciences laccase hydrophobin graphene |
| title | From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme |
| title_full | From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme |
| title_fullStr | From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme |
| title_full_unstemmed | From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme |
| title_short | From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme |
| title_sort | from graphite to laccase biofunctionalized few layer graphene a one pot approach using a chimeric enzyme |
| topic | laccase hydrophobin graphene |
| url | https://www.mdpi.com/1422-0067/21/11/3741 |
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