Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment
ABSTRACT: Vaccination is one of the most effective strategies for preventing infectious diseases but individual vaccine responses are highly heterogeneous. Host genetics and gut microbiota composition are 2 likely drivers of this heterogeneity. We studied 94 animals belonging to 4 lines of laying he...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001883 |
| _version_ | 1797235589069668352 |
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| author | Alexandre Lecoeur Fany Blanc David Gourichon Nicolas Bruneau Thierry Burlot Marie-Hélène Pinard-van der Laan Fanny Calenge |
| author_facet | Alexandre Lecoeur Fany Blanc David Gourichon Nicolas Bruneau Thierry Burlot Marie-Hélène Pinard-van der Laan Fanny Calenge |
| author_sort | Alexandre Lecoeur |
| collection | DOAJ |
| description | ABSTRACT: Vaccination is one of the most effective strategies for preventing infectious diseases but individual vaccine responses are highly heterogeneous. Host genetics and gut microbiota composition are 2 likely drivers of this heterogeneity. We studied 94 animals belonging to 4 lines of laying hens: a White Leghorn experimental line genetically selected for a high antibody response against the Newcastle Disease Virus (NDV) vaccine (ND3) and its unselected control line (CTR), and 2 commercial lines (White Leghorn [LEG] and Rhode Island Red [RIR]). Animals were reared in the same conditions from hatching to 42 d of age, and animals from different genetic lines were mixed. Animals were vaccinated at 22 d of age and their humoral vaccine response against NDV was assessed by hemagglutination inhibition assay and ELISA from blood samples collected at 15, 19, and 21 d after vaccination. The immune parameters studied were the 3 immunoglobulins subtypes A, M, and Y and the blood cell composition was assessed by flow cytometry. The composition of the cecal microbiota was assessed at the end of the experiment by analyzing amplified 16S rRNA gene sequences to obtain amplicon sequence variants (ASV). The 4 lines showed significantly different levels of NDV vaccine response at the 3 measured points, with, logically, a higher response of the genetically selected ND3 line, and intermediate and low responses for the unselected CTR control line and for the 2 commercial lines, respectively. The ND3 line displayed also a higher proportion of immunoglobulins (IgA, IgM, and IgY). The RIR line showed the most different blood cell composition. The 4 lines showed significantly different microbiota characteristics: composition, abundances at all taxonomic levels, and correlations between genera and vaccine response. The tested genetic lines differ for immune parameters and gut microbiota composition and functions. These phenotypic differences can be attributed to genetic differences between lines. Causal relationships between both types of parameters are discussed and will be investigated in further studies. |
| first_indexed | 2024-04-24T16:50:21Z |
| format | Article |
| id | doaj.art-24fb9ce10bd6492888eddc33072211dc |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T16:50:21Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-24fb9ce10bd6492888eddc33072211dc2024-03-29T05:49:28ZengElsevierPoultry Science0032-57912024-05-011035103609Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environmentAlexandre Lecoeur0Fany Blanc1David Gourichon2Nicolas Bruneau3Thierry Burlot4Marie-Hélène Pinard-van der Laan5Fanny Calenge6Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas 78350, France; Corresponding author:Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas 78350, FranceINRAE, PEAT, Nouzilly 37380, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas 78350, FranceNOVOGEN, Plédran 22960, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas 78350, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas 78350, FranceABSTRACT: Vaccination is one of the most effective strategies for preventing infectious diseases but individual vaccine responses are highly heterogeneous. Host genetics and gut microbiota composition are 2 likely drivers of this heterogeneity. We studied 94 animals belonging to 4 lines of laying hens: a White Leghorn experimental line genetically selected for a high antibody response against the Newcastle Disease Virus (NDV) vaccine (ND3) and its unselected control line (CTR), and 2 commercial lines (White Leghorn [LEG] and Rhode Island Red [RIR]). Animals were reared in the same conditions from hatching to 42 d of age, and animals from different genetic lines were mixed. Animals were vaccinated at 22 d of age and their humoral vaccine response against NDV was assessed by hemagglutination inhibition assay and ELISA from blood samples collected at 15, 19, and 21 d after vaccination. The immune parameters studied were the 3 immunoglobulins subtypes A, M, and Y and the blood cell composition was assessed by flow cytometry. The composition of the cecal microbiota was assessed at the end of the experiment by analyzing amplified 16S rRNA gene sequences to obtain amplicon sequence variants (ASV). The 4 lines showed significantly different levels of NDV vaccine response at the 3 measured points, with, logically, a higher response of the genetically selected ND3 line, and intermediate and low responses for the unselected CTR control line and for the 2 commercial lines, respectively. The ND3 line displayed also a higher proportion of immunoglobulins (IgA, IgM, and IgY). The RIR line showed the most different blood cell composition. The 4 lines showed significantly different microbiota characteristics: composition, abundances at all taxonomic levels, and correlations between genera and vaccine response. The tested genetic lines differ for immune parameters and gut microbiota composition and functions. These phenotypic differences can be attributed to genetic differences between lines. Causal relationships between both types of parameters are discussed and will be investigated in further studies.http://www.sciencedirect.com/science/article/pii/S0032579124001883geneticgallus gallusgut microbiotavaccine responseimmunity |
| spellingShingle | Alexandre Lecoeur Fany Blanc David Gourichon Nicolas Bruneau Thierry Burlot Marie-Hélène Pinard-van der Laan Fanny Calenge Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment Poultry Science genetic gallus gallus gut microbiota vaccine response immunity |
| title | Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| title_full | Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| title_fullStr | Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| title_full_unstemmed | Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| title_short | Host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| title_sort | host genetics drives differences in cecal microbiota composition and immune traits of laying hens raised in the same environment |
| topic | genetic gallus gallus gut microbiota vaccine response immunity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001883 |
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