RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR
Abstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi g...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08813-1 |
| _version_ | 1830286520080138240 |
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| author | Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli |
| author_facet | Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli |
| author_sort | Thais B. Rodrigues |
| collection | DOAJ |
| description | Abstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsRBP) as well as for proteins involved in dsRNA transport and the systemic RNAi. We also compared expression of six potential reference genes that could be used to normalize gene expression and selected gapdh and rpl32 as the most reliable genes among different tissues and stages of ALB. Injection of double-stranded RNA (dsRNA) targeting gene coding for inhibitor of apoptosis (IAP) into larvae and adults resulted in a significant knockdown of this gene and caused the death of 90% of the larvae and 100% of adults. No mortality of both larvae and adults injected with dsRNA targeting gene coding for green fluorescence protein (GFP, as a negative control) was observed. These data suggest that functional RNAi machinery exists in ALB and a potential RNAi-based method could be developed for controlling this insect. |
| first_indexed | 2024-12-19T04:00:49Z |
| format | Article |
| id | doaj.art-24fd3910853f427c9f28f7ec502bc800 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:00:49Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-24fd3910853f427c9f28f7ec502bc8002022-12-21T20:36:41ZengNature PortfolioScientific Reports2045-23222017-08-017111010.1038/s41598-017-08813-1RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCRThais B. Rodrigues0Ramesh Kumar Dhandapani1Jian J. Duan2Subba Reddy Palli3Department of Entomology, University of Kentucky, College of Agriculture, Food and EnvironmentDepartment of Entomology, University of Kentucky, College of Agriculture, Food and EnvironmentUSDA ARS Beneficial Insects Introduction Research UnitDepartment of Entomology, University of Kentucky, College of Agriculture, Food and EnvironmentAbstract Asian Longhorned Beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries including the United States, Canada, and Europe. RNA interference (RNAi) technology is being developed as a novel method for pest management. Here, we identified the ALB core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsRBP) as well as for proteins involved in dsRNA transport and the systemic RNAi. We also compared expression of six potential reference genes that could be used to normalize gene expression and selected gapdh and rpl32 as the most reliable genes among different tissues and stages of ALB. Injection of double-stranded RNA (dsRNA) targeting gene coding for inhibitor of apoptosis (IAP) into larvae and adults resulted in a significant knockdown of this gene and caused the death of 90% of the larvae and 100% of adults. No mortality of both larvae and adults injected with dsRNA targeting gene coding for green fluorescence protein (GFP, as a negative control) was observed. These data suggest that functional RNAi machinery exists in ALB and a potential RNAi-based method could be developed for controlling this insect.https://doi.org/10.1038/s41598-017-08813-1 |
| spellingShingle | Thais B. Rodrigues Ramesh Kumar Dhandapani Jian J. Duan Subba Reddy Palli RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR Scientific Reports |
| title | RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR |
| title_full | RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR |
| title_fullStr | RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR |
| title_full_unstemmed | RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR |
| title_short | RNA interference in the Asian Longhorned Beetle:Identification of Key RNAi Genes and Reference Genes for RT-qPCR |
| title_sort | rna interference in the asian longhorned beetle identification of key rnai genes and reference genes for rt qpcr |
| url | https://doi.org/10.1038/s41598-017-08813-1 |
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