Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury
This study was designed to compare the effects of various doses of botulinum neurotoxin A (BoNT/A) on nerve regeneration. Sixty-five six-week-old rats with sciatic nerve injury were randomly allocated to three experimental groups, a control group, and a sham group. The experimental groups received a...
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MDPI AG
2023-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/12/691 |
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| author | Seokjoon Hwang Minsu Seo Tae Heon Lee Ho Jun Lee Jin-woo Park Bum Sun Kwon Kiyeun Nam |
| author_facet | Seokjoon Hwang Minsu Seo Tae Heon Lee Ho Jun Lee Jin-woo Park Bum Sun Kwon Kiyeun Nam |
| author_sort | Seokjoon Hwang |
| collection | DOAJ |
| description | This study was designed to compare the effects of various doses of botulinum neurotoxin A (BoNT/A) on nerve regeneration. Sixty-five six-week-old rats with sciatic nerve injury were randomly allocated to three experimental groups, a control group, and a sham group. The experimental groups received a single session of intraneural BoNT/A (3.5, 7.0, or 14 U/kg) injection immediately after nerve-crushing injury. The control group received normal intraneural saline injections after sciatic nerve injury. At three, six, and nine weeks after nerve damage, immunofluorescence staining, an ELISA, and toluidine blue staining was used to evaluate the regenerated nerves. Serial sciatic functional index analyses and electrophysiological tests were performed every week for nine weeks. A higher expression of GFAP, S100β, GAP43, NF200, BDNF, and NGF was seen in the 3.5 U/kg and 7.0 U/kg BoNT/A groups. The average area and myelin thickness were significantly greater in the 3.5 U/kg and 7.0 U/kg BoNT/A groups. The sciatic functional index and compound muscle action potential amplitudes exhibited similar trends. These findings indicate that the 3.5 U/kg and 7.0 U/kg BoNT/A groups exhibited better nerve regeneration than the 14 U/kg BoNT/A and control group. As the 3.5 U/kg and the 7.0 U/kg BoNT/A groups exhibited no statistical difference, we recommend using 3.5 U/kg BoNT/A for its cost-effectiveness. |
| first_indexed | 2024-03-08T20:18:52Z |
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| language | English |
| last_indexed | 2024-03-08T20:18:52Z |
| publishDate | 2023-12-01 |
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| series | Toxins |
| spelling | doaj.art-24fd592b00854cd2bbfee317522103b42023-12-22T14:46:17ZengMDPI AGToxins2072-66512023-12-01151269110.3390/toxins15120691Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve InjurySeokjoon Hwang0Minsu Seo1Tae Heon Lee2Ho Jun Lee3Jin-woo Park4Bum Sun Kwon5Kiyeun Nam6Department of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaDepartment of Physical Medicine & Rehabilitation, Dongguk University College of Medicine, Goyang 10326, Republic of KoreaThis study was designed to compare the effects of various doses of botulinum neurotoxin A (BoNT/A) on nerve regeneration. Sixty-five six-week-old rats with sciatic nerve injury were randomly allocated to three experimental groups, a control group, and a sham group. The experimental groups received a single session of intraneural BoNT/A (3.5, 7.0, or 14 U/kg) injection immediately after nerve-crushing injury. The control group received normal intraneural saline injections after sciatic nerve injury. At three, six, and nine weeks after nerve damage, immunofluorescence staining, an ELISA, and toluidine blue staining was used to evaluate the regenerated nerves. Serial sciatic functional index analyses and electrophysiological tests were performed every week for nine weeks. A higher expression of GFAP, S100β, GAP43, NF200, BDNF, and NGF was seen in the 3.5 U/kg and 7.0 U/kg BoNT/A groups. The average area and myelin thickness were significantly greater in the 3.5 U/kg and 7.0 U/kg BoNT/A groups. The sciatic functional index and compound muscle action potential amplitudes exhibited similar trends. These findings indicate that the 3.5 U/kg and 7.0 U/kg BoNT/A groups exhibited better nerve regeneration than the 14 U/kg BoNT/A and control group. As the 3.5 U/kg and the 7.0 U/kg BoNT/A groups exhibited no statistical difference, we recommend using 3.5 U/kg BoNT/A for its cost-effectiveness.https://www.mdpi.com/2072-6651/15/12/691botulinum toxinperipheral nerve injuryneural regenerationfunctional recovery |
| spellingShingle | Seokjoon Hwang Minsu Seo Tae Heon Lee Ho Jun Lee Jin-woo Park Bum Sun Kwon Kiyeun Nam Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury Toxins botulinum toxin peripheral nerve injury neural regeneration functional recovery |
| title | Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_full | Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_fullStr | Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_full_unstemmed | Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_short | Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury |
| title_sort | comparison of the effects of botulinum toxin doses on nerve regeneration in rats with experimentally induced sciatic nerve injury |
| topic | botulinum toxin peripheral nerve injury neural regeneration functional recovery |
| url | https://www.mdpi.com/2072-6651/15/12/691 |
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