CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study
Background Urothelial carcinoma (UC) of the bladder is a major cause of morbidity and mortality throughout the world. The present study was carried out to see the expression of CD105 and CD34 as marker of angiogenesis in UC and study their relationship with grades and muscle invasion. Methods 50 ca...
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| Format: | Article |
| Language: | English |
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Asian Medical Press Ltd.(H.K.)
2023-07-01
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| Series: | Annals of Urologic Oncology |
| Subjects: | |
| Online Access: | http://auo.asmepress.com/articles/new-24-29.html |
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| author | Sheenam Azad Aditi Bisht Avneesh Malviya Jyotsna Bhateja Seema Acharya |
| author_facet | Sheenam Azad Aditi Bisht Avneesh Malviya Jyotsna Bhateja Seema Acharya |
| author_sort | Sheenam Azad |
| collection | DOAJ |
| description | Background Urothelial carcinoma (UC) of the bladder is a major cause of morbidity and mortality throughout the world. The present study was carried out to see the expression of CD105 and CD34 as marker of angiogenesis in UC and study their relationship with grades and muscle invasion.
Methods 50 cases of histopathologically diagnosed UC were included. CD105 and CD34 IHC staining was performed. Microvessel density (MVD) evaluation by CD105 and CD34 was compared. The number of microvessels were counted in high power in three hot spots. The highest value obtained amongst three fields were reported as MVD. Statistical analysis was done by (SPSS 28.00 version for windows) using independent t-test.
Results There were 45 high grade cases UC 5 low grade cases of UC. Invasive papillary UC was seen in 25 cases (50%) followed by invasive UC seen in 13 cases (26%). Both Lamina propria (LP) invasion and muscularis propria invasion were noted in 16 cases of high-grade urothelial carcinoma. Only LP invasion was seen in 3 cases of low grade and 25 cases of high-grade UC respectively. Evaluation of MVD CD105 and MVD CD34 did not show any significant association of MVD with grade with a p value of 0.214 & 0.429 respectively. Aso there was no significant association of MVD CD105 (p=0.580) & CD34 (p=0.747) with muscularis propria invasion. Overall mean CD105 and CD34 were similar with no statistical difference between the two markers.
Conclusion Both CD105 and CD34 can be used as marker to assess angiogenesis. However, there is no significant association of MVD with grade or muscle invasion in UC. |
| first_indexed | 2024-03-12T22:27:24Z |
| format | Article |
| id | doaj.art-24ff8f161bf741ef895064418c13e666 |
| institution | Directory Open Access Journal |
| issn | 2617-7765 2617-7773 |
| language | English |
| last_indexed | 2024-03-12T22:27:24Z |
| publishDate | 2023-07-01 |
| publisher | Asian Medical Press Ltd.(H.K.) |
| record_format | Article |
| series | Annals of Urologic Oncology |
| spelling | doaj.art-24ff8f161bf741ef895064418c13e6662023-07-22T01:30:57ZengAsian Medical Press Ltd.(H.K.)Annals of Urologic Oncology2617-77652617-77732023-07-01610.32948/auo.2023.07.10CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional StudySheenam Azad0Aditi Bisht1 Avneesh Malviya2Jyotsna Bhateja3Seema Acharya4Department of Pathology, Shri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun, Uttarakhand, India.Department of Pathology, Shri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun, Uttarakhand, India.Department of Pathology, Government Doon Medical College, Dehradun, Uttarakhand, India.Department of Pathology, Shri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun, Uttarakhand, India.Department of Pathology, Shri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun, Uttarakhand, India.Background Urothelial carcinoma (UC) of the bladder is a major cause of morbidity and mortality throughout the world. The present study was carried out to see the expression of CD105 and CD34 as marker of angiogenesis in UC and study their relationship with grades and muscle invasion. Methods 50 cases of histopathologically diagnosed UC were included. CD105 and CD34 IHC staining was performed. Microvessel density (MVD) evaluation by CD105 and CD34 was compared. The number of microvessels were counted in high power in three hot spots. The highest value obtained amongst three fields were reported as MVD. Statistical analysis was done by (SPSS 28.00 version for windows) using independent t-test. Results There were 45 high grade cases UC 5 low grade cases of UC. Invasive papillary UC was seen in 25 cases (50%) followed by invasive UC seen in 13 cases (26%). Both Lamina propria (LP) invasion and muscularis propria invasion were noted in 16 cases of high-grade urothelial carcinoma. Only LP invasion was seen in 3 cases of low grade and 25 cases of high-grade UC respectively. Evaluation of MVD CD105 and MVD CD34 did not show any significant association of MVD with grade with a p value of 0.214 & 0.429 respectively. Aso there was no significant association of MVD CD105 (p=0.580) & CD34 (p=0.747) with muscularis propria invasion. Overall mean CD105 and CD34 were similar with no statistical difference between the two markers. Conclusion Both CD105 and CD34 can be used as marker to assess angiogenesis. However, there is no significant association of MVD with grade or muscle invasion in UC.http://auo.asmepress.com/articles/new-24-29.htmlcd105cd34microvessel densitybladder cancer |
| spellingShingle | Sheenam Azad Aditi Bisht Avneesh Malviya Jyotsna Bhateja Seema Acharya CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study Annals of Urologic Oncology cd105 cd34 microvessel density bladder cancer |
| title | CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study |
| title_full | CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study |
| title_fullStr | CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study |
| title_full_unstemmed | CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study |
| title_short | CD105 and CD34 as Markers of Angiogenesis of Urothelial Carcinoma: A Cross Sectional Study |
| title_sort | cd105 and cd34 as markers of angiogenesis of urothelial carcinoma a cross sectional study |
| topic | cd105 cd34 microvessel density bladder cancer |
| url | http://auo.asmepress.com/articles/new-24-29.html |
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