Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia
The present study was undertaken to evaluate [Ca2+]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. The [Ca2+]i reached after the extracellular c...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/2018/02/responsiveness-to-progesterone-and-potassium-channel-blockers-4-aminopyridine-tetraethylammonium-and-free-ca2-contentration-in-spermatozoa-of-patients-with-oligozoospermialeucocytospermia.html |
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| author | R. V. Fafula G. V. Danylovych A. S. Besedina O. V. Melnyk Z. D. Vorobets |
| author_facet | R. V. Fafula G. V. Danylovych A. S. Besedina O. V. Melnyk Z. D. Vorobets |
| author_sort | R. V. Fafula |
| collection | DOAJ |
| description | The present study was undertaken to evaluate [Ca2+]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. The [Ca2+]i reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. There were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [Ca2+]i. was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. The 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [Ca2+]i in normo- and pathozoospermic samples. The mechanisms of progesterone-dependent activation of the Ca2+-entry and the functioning of the voltage gated Ca2+-channels of plasmalemma are disturbed in pathologies – there was no increase in the Ca2+ level in the conditions of K+-depolarization (in the presence of inhibitors of K+-channels). |
| first_indexed | 2024-03-09T07:29:57Z |
| format | Article |
| id | doaj.art-2502b2ae15b14c43a4b5e927b5d4c645 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:29:57Z |
| publishDate | 2018-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-2502b2ae15b14c43a4b5e927b5d4c6452023-12-03T06:27:16ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032018-02-01901485710.15407/ubj90.01.048Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermiaR. V. Fafula0G. V. Danylovych1A. S. Besedina2O. V. Melnyk3Z. D. Vorobets4Danylo Halytsky Lviv National Medical University, Ukraine;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;Danylo Halytsky Lviv National Medical University, Ukraine;Danylo Halytsky Lviv National Medical University, Ukraine;Danylo Halytsky Lviv National Medical University, Ukraine;The present study was undertaken to evaluate [Ca2+]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. The [Ca2+]i reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. There were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [Ca2+]i. was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. The 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [Ca2+]i in normo- and pathozoospermic samples. The mechanisms of progesterone-dependent activation of the Ca2+-entry and the functioning of the voltage gated Ca2+-channels of plasmalemma are disturbed in pathologies – there was no increase in the Ca2+ level in the conditions of K+-depolarization (in the presence of inhibitors of K+-channels).http://ukrbiochemjournal.org/2018/02/responsiveness-to-progesterone-and-potassium-channel-blockers-4-aminopyridine-tetraethylammonium-and-free-ca2-contentration-in-spermatozoa-of-patients-with-oligozoospermialeucocytospermia.htmlcalciuminhibitors of K(+) channelsprogesteronespermatozoa |
| spellingShingle | R. V. Fafula G. V. Danylovych A. S. Besedina O. V. Melnyk Z. D. Vorobets Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia The Ukrainian Biochemical Journal calcium inhibitors of K(+) channels progesterone spermatozoa |
| title | Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia |
| title_full | Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia |
| title_fullStr | Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia |
| title_full_unstemmed | Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia |
| title_short | Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia |
| title_sort | responsiveness to progesterone and potassium channel blockers 4 aminopyridine tetraethylammonium and free ca 2 contentration in spermatozoa of patients with oligozoospermia leucocytospermia |
| topic | calcium inhibitors of K(+) channels progesterone spermatozoa |
| url | http://ukrbiochemjournal.org/2018/02/responsiveness-to-progesterone-and-potassium-channel-blockers-4-aminopyridine-tetraethylammonium-and-free-ca2-contentration-in-spermatozoa-of-patients-with-oligozoospermialeucocytospermia.html |
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