Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis.
Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and pro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3397951?pdf=render |
| _version_ | 1831678542866284544 |
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| author | Federico Carlos Blanco Marcelo Soria María Verónica Bianco Fabiana Bigi |
| author_facet | Federico Carlos Blanco Marcelo Soria María Verónica Bianco Fabiana Bigi |
| author_sort | Federico Carlos Blanco |
| collection | DOAJ |
| description | Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change >2.5 and <-2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis. |
| first_indexed | 2024-12-20T05:00:38Z |
| format | Article |
| id | doaj.art-25045851bb074c0b883cfd90e9b2c544 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T05:00:38Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-25045851bb074c0b883cfd90e9b2c5442022-12-21T19:52:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4106610.1371/journal.pone.0041066Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis.Federico Carlos BlancoMarcelo SoriaMaría Verónica BiancoFabiana BigiMycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change >2.5 and <-2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.http://europepmc.org/articles/PMC3397951?pdf=render |
| spellingShingle | Federico Carlos Blanco Marcelo Soria María Verónica Bianco Fabiana Bigi Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. PLoS ONE |
| title | Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. |
| title_full | Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. |
| title_fullStr | Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. |
| title_full_unstemmed | Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. |
| title_short | Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis. |
| title_sort | transcriptional response of peripheral blood mononuclear cells from cattle infected with mycobacterium bovis |
| url | http://europepmc.org/articles/PMC3397951?pdf=render |
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