The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis
BackgroundThe long non-coding RNA (lncRNA) RP9 pseudogene (RP9P) is a pseudogene-derived lncRNA that has never been reported in cancer, and its function underlying tumorigenesis in colorectal cancer (CRC) remains unknown.MethodsRP9P and miR-133a-3p were filtered through bioinformatics analysis. The...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.843064/full |
| _version_ | 1818195536913629184 |
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| author | Zhichao Jin Zhichao Jin Baoxinzi Liu Baoxinzi Liu Bofan Lin Bofan Lin Ran Yang Ran Yang Cunen Wu Cunen Wu Weiwei Xue Weiwei Xue Xi Zou Xi Zou Jun Qian Jun Qian |
| author_facet | Zhichao Jin Zhichao Jin Baoxinzi Liu Baoxinzi Liu Bofan Lin Bofan Lin Ran Yang Ran Yang Cunen Wu Cunen Wu Weiwei Xue Weiwei Xue Xi Zou Xi Zou Jun Qian Jun Qian |
| author_sort | Zhichao Jin |
| collection | DOAJ |
| description | BackgroundThe long non-coding RNA (lncRNA) RP9 pseudogene (RP9P) is a pseudogene-derived lncRNA that has never been reported in cancer, and its function underlying tumorigenesis in colorectal cancer (CRC) remains unknown.MethodsRP9P and miR-133a-3p were filtered through bioinformatics analysis. The level of RP9P, miR-133a-3p, and FOXQ1 in CRC cell lines was detected by real-time PCR. Cell Counting Kit-8 and flow cytometric analyses were used to detect cell proliferation and apoptosis, respectively. Interactions between RP9P, miR-133a-3p, and FOXQ1 were confirmed by a dual-luciferase reporter assay.ResultsRP9P was overexpressed in CRC compared to normal control tissues and cells. Knockdown of RP9P inhibited CRC cell viability. RP9P directly interacted with miR-133a-3p, and miR-133a-3p downregulation abrogated the tumor-suppressing effect of RP9P knockdown. miR-133a-3p directly targeted FOXQ, which was positively regulated by RP9P. RP9P knockdown decreased FOXQ1 expression levels in CRC cells by directly targeting miR-133a-3p via a sponge mechanism. In addition, in vivo experiments in a xenograft model revealed that downregulated RP9P expression inhibited CRC cell tumorigenesis.ConclusionRP9P promotes colorectal cancer progression by regulating the miR-133a-3p/FOXQ1 axis. |
| first_indexed | 2024-12-12T01:19:45Z |
| format | Article |
| id | doaj.art-2504e3080d384391b90b48d550f2d112 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T01:19:45Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-2504e3080d384391b90b48d550f2d1122022-12-22T00:43:16ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-05-011210.3389/fonc.2022.843064843064The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 AxisZhichao Jin0Zhichao Jin1Baoxinzi Liu2Baoxinzi Liu3Bofan Lin4Bofan Lin5Ran Yang6Ran Yang7Cunen Wu8Cunen Wu9Weiwei Xue10Weiwei Xue11Xi Zou12Xi Zou13Jun Qian14Jun Qian15Department of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaDepartment of Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Collaborative Innovation Center of TCM Cancer Prevention and Treatment, Nanjing, ChinaBackgroundThe long non-coding RNA (lncRNA) RP9 pseudogene (RP9P) is a pseudogene-derived lncRNA that has never been reported in cancer, and its function underlying tumorigenesis in colorectal cancer (CRC) remains unknown.MethodsRP9P and miR-133a-3p were filtered through bioinformatics analysis. The level of RP9P, miR-133a-3p, and FOXQ1 in CRC cell lines was detected by real-time PCR. Cell Counting Kit-8 and flow cytometric analyses were used to detect cell proliferation and apoptosis, respectively. Interactions between RP9P, miR-133a-3p, and FOXQ1 were confirmed by a dual-luciferase reporter assay.ResultsRP9P was overexpressed in CRC compared to normal control tissues and cells. Knockdown of RP9P inhibited CRC cell viability. RP9P directly interacted with miR-133a-3p, and miR-133a-3p downregulation abrogated the tumor-suppressing effect of RP9P knockdown. miR-133a-3p directly targeted FOXQ, which was positively regulated by RP9P. RP9P knockdown decreased FOXQ1 expression levels in CRC cells by directly targeting miR-133a-3p via a sponge mechanism. In addition, in vivo experiments in a xenograft model revealed that downregulated RP9P expression inhibited CRC cell tumorigenesis.ConclusionRP9P promotes colorectal cancer progression by regulating the miR-133a-3p/FOXQ1 axis.https://www.frontiersin.org/articles/10.3389/fonc.2022.843064/fullcolorectal cancerRP9PmiR-133a-3pceRNAlncRNA |
| spellingShingle | Zhichao Jin Zhichao Jin Baoxinzi Liu Baoxinzi Liu Bofan Lin Bofan Lin Ran Yang Ran Yang Cunen Wu Cunen Wu Weiwei Xue Weiwei Xue Xi Zou Xi Zou Jun Qian Jun Qian The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis Frontiers in Oncology colorectal cancer RP9P miR-133a-3p ceRNA lncRNA |
| title | The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis |
| title_full | The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis |
| title_fullStr | The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis |
| title_full_unstemmed | The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis |
| title_short | The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis |
| title_sort | novel lncrna rp9p promotes colorectal cancer progression by modulating mir 133a 3p foxq1 axis |
| topic | colorectal cancer RP9P miR-133a-3p ceRNA lncRNA |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.843064/full |
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