Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L.
Background. The knowledge of genetic control of vernalization response in the ultra-early accessions can facilitate bread wheat breeding for a high adaptive capacity. Materials and methods. The study involved the ultra-early lines Rico (k-65588) and Rimax (k-67257) as the earliest maturing lines in...
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| Format: | Article |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2022-01-01
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| Series: | Биотехнология и селекция растений |
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| Online Access: | https://biosel.elpub.ru/jour/article/view/128 |
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| author | B. V. Rigin E. V. Zuev I. I Matvienko A. S. Andreeva |
| author_facet | B. V. Rigin E. V. Zuev I. I Matvienko A. S. Andreeva |
| author_sort | B. V. Rigin |
| collection | DOAJ |
| description | Background. The knowledge of genetic control of vernalization response in the ultra-early accessions can facilitate bread wheat breeding for a high adaptive capacity. Materials and methods. The study involved the ultra-early lines Rico (k-65588) and Rimax (k-67257) as the earliest maturing lines in the VIR bread wheat collection, as well as 10 Rifor lines (k-67120, k-67121, k-67250-67256) with a high rate of development before heading. A late ripening accession ‘Forlani Roberto’ (k-42641) and ‘Leningradskaya 6’ variety (k-64900), regionally adapted to Northwestern Russia, were also studied. The alleles of the Vrn and Ppd genes were identified by the PCR analysis using the allele-specific primers published in literature sources. The response to vernalization (30 days at 3°C) and a short 12-hour day were determined using a methodology accepted at VIR. Results. The ultra-early lines respond to a short 12-hour day and 30-day vernalization very poorly. The genotype of ultra-early wheat lines is mainly represented by three genes, Vrn-A1, Vrn-B1a, and Vrn-D1, which ensure insensitivity to vernalization alongside with the expression of Ppd-D1a, which controls the response to photoperiod. The ultra-early lines Rifor 4 and Rifor 5 have a recessive allele vrn-A1a, like the original ‘Forlani Roberto’ accession. The lines Rifor 4 and Rifor 5 are vernalization-insensitive under the long day and have a very weak response under the short day (3.5±0.42 days and 4.0±0.61 days, respectively). However, ‘Forlani Roberto’ with the vrn-A1a gene responds to vernalization in the same way under any photoperiod (12.3±1.58 days and 12.2±0.74 days). Conclusion The ultra-early lines of bread wheat Rifor 4 and Rifor 5 with the vrn-A1a gene can have no response to vernalization or have a low level response. This effect can be a reason for the formation of a complex of modifier genes along with the dominant gene Vrn-D1, which forms during the hybridization of F7-8 Rico × Forlani Roberto. The ultra-early lines of bread wheat Rico, Rimax and Rifor (k-67120, k-67121, k-67250-67256) can serve as effective sources of genes for earliness in common wheat breeding. |
| first_indexed | 2024-03-08T05:52:24Z |
| format | Article |
| id | doaj.art-25081303b88746ceb65e0f06b5751173 |
| institution | Directory Open Access Journal |
| issn | 2658-6266 2658-6258 |
| language | English |
| last_indexed | 2024-03-08T05:52:24Z |
| publishDate | 2022-01-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Биотехнология и селекция растений |
| spelling | doaj.art-25081303b88746ceb65e0f06b57511732024-02-05T06:53:48ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesБиотехнология и селекция растений2658-62662658-62582022-01-0143263610.30901/2658-6266-2021-3-o284Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L.B. V. Rigin0E. V. Zuev1I. I Matvienko2A. S. Andreeva3N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesBackground. The knowledge of genetic control of vernalization response in the ultra-early accessions can facilitate bread wheat breeding for a high adaptive capacity. Materials and methods. The study involved the ultra-early lines Rico (k-65588) and Rimax (k-67257) as the earliest maturing lines in the VIR bread wheat collection, as well as 10 Rifor lines (k-67120, k-67121, k-67250-67256) with a high rate of development before heading. A late ripening accession ‘Forlani Roberto’ (k-42641) and ‘Leningradskaya 6’ variety (k-64900), regionally adapted to Northwestern Russia, were also studied. The alleles of the Vrn and Ppd genes were identified by the PCR analysis using the allele-specific primers published in literature sources. The response to vernalization (30 days at 3°C) and a short 12-hour day were determined using a methodology accepted at VIR. Results. The ultra-early lines respond to a short 12-hour day and 30-day vernalization very poorly. The genotype of ultra-early wheat lines is mainly represented by three genes, Vrn-A1, Vrn-B1a, and Vrn-D1, which ensure insensitivity to vernalization alongside with the expression of Ppd-D1a, which controls the response to photoperiod. The ultra-early lines Rifor 4 and Rifor 5 have a recessive allele vrn-A1a, like the original ‘Forlani Roberto’ accession. The lines Rifor 4 and Rifor 5 are vernalization-insensitive under the long day and have a very weak response under the short day (3.5±0.42 days and 4.0±0.61 days, respectively). However, ‘Forlani Roberto’ with the vrn-A1a gene responds to vernalization in the same way under any photoperiod (12.3±1.58 days and 12.2±0.74 days). Conclusion The ultra-early lines of bread wheat Rifor 4 and Rifor 5 with the vrn-A1a gene can have no response to vernalization or have a low level response. This effect can be a reason for the formation of a complex of modifier genes along with the dominant gene Vrn-D1, which forms during the hybridization of F7-8 Rico × Forlani Roberto. The ultra-early lines of bread wheat Rico, Rimax and Rifor (k-67120, k-67121, k-67250-67256) can serve as effective sources of genes for earliness in common wheat breeding.https://biosel.elpub.ru/jour/article/view/128spring bread wheatultra-earlinessrifor linesvernalization responsephotoperiod<i>vrn</i> and <i>ppd</i> gene allelespcr analysisbreeding |
| spellingShingle | B. V. Rigin E. V. Zuev I. I Matvienko A. S. Andreeva Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. Биотехнология и селекция растений spring bread wheat ultra-earliness rifor lines vernalization response photoperiod <i>vrn</i> and <i>ppd</i> gene alleles pcr analysis breeding |
| title | Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. |
| title_full | Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. |
| title_fullStr | Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. |
| title_full_unstemmed | Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. |
| title_short | Molecular labeling of <i>Vrn</i>, <i>Ppd</i> genes and vernalization response of the ultra-early lines of spring bread wheat <i>Triticum aestivum</i> L. |
| title_sort | molecular labeling of i vrn i i ppd i genes and vernalization response of the ultra early lines of spring bread wheat i triticum aestivum i l |
| topic | spring bread wheat ultra-earliness rifor lines vernalization response photoperiod <i>vrn</i> and <i>ppd</i> gene alleles pcr analysis breeding |
| url | https://biosel.elpub.ru/jour/article/view/128 |
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