Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study
As an inflammatory disease of the lung, asthma is characterized by bronchoconstriction, mucus hypersecretion, inflammatory mediator release, and eosinophil recruitment. Cupping therapy is an ancient method of treatment for a vast range of ailments. This study aimed to evaluate the anti-asthmatic ef...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-03-01
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| Series: | Traditional and Integrative Medicine |
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| Online Access: | https://jtim.tums.ac.ir/index.php/jtim/article/view/404 |
| _version_ | 1819060538680803328 |
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| author | Abbas Joushan Sadegh Rajabi Khosrow Agin Mohammad Hossein Ayati Fatemeh Jafari Babak Daneshfard Sayyed Shamsadin Athari Zeinab Ghahremani Rasool Choopani |
| author_facet | Abbas Joushan Sadegh Rajabi Khosrow Agin Mohammad Hossein Ayati Fatemeh Jafari Babak Daneshfard Sayyed Shamsadin Athari Zeinab Ghahremani Rasool Choopani |
| author_sort | Abbas Joushan |
| collection | DOAJ |
| description |
As an inflammatory disease of the lung, asthma is characterized by bronchoconstriction, mucus hypersecretion, inflammatory mediator release, and eosinophil recruitment. Cupping therapy is an ancient method of treatment for a vast range of ailments. This study aimed to evaluate the anti-asthmatic effects of wet cupping therapy (WCT) in a mouse model. A total number of 35 Balb/c mice were randomly divided into five groups (n = 7): Negative and positive control groups were administered Phosphate Buffered Saline (PBS) and ovalbumin (OVA), respectively. The remaining three OVA-challenged groups were treated with budesonide, one session, and two sessions of WCT. Finally, eosinophil counts, the gene expressions, and the protein levels of interleukins IL-5, -13, and -33 were measured in bronchoalveolar lavage fluid (BALF) of mice. Lung tissues were removed and kept for histopathological evaluations. Both eosinophil counts and interleukin levels in BALFs were significantly diminished following WCT. Moreover, WCT prevented hyperplastic growth of goblet cells, overproduction of mucus, and inflammation of peribronchial and perivascular areas of lung tissue of mice compared to positive control group. Interestingly, the anti-inflammatory effects of WCT against asthma were comparable to budesonide. Our data suggested that the anti-asthmatic effects of WCT were mediated by reducing eosinophil trafficking and modulating Th2 inflammatory cytokines, leading to the histological changes of the lung. This may propose WCT as an efficient therapeutic approach to mitigate inflammatory complications of asthma.
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| first_indexed | 2024-12-21T14:28:35Z |
| format | Article |
| id | doaj.art-2508ea8c4a744317ae6199c1fda4b627 |
| institution | Directory Open Access Journal |
| issn | 2476-5104 2476-5112 |
| language | English |
| last_indexed | 2024-12-21T14:28:35Z |
| publishDate | 2022-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Traditional and Integrative Medicine |
| spelling | doaj.art-2508ea8c4a744317ae6199c1fda4b6272022-12-21T19:00:34ZengTehran University of Medical SciencesTraditional and Integrative Medicine2476-51042476-51122022-03-017110.18502/tim.v7i1.9062Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological StudyAbbas Joushan0Sadegh Rajabi1Khosrow Agin2Mohammad Hossein Ayati3Fatemeh Jafari4Babak Daneshfard5Sayyed Shamsadin Athari6Zeinab Ghahremani7Rasool Choopani8Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, IranTraditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, IranPulmonologist and Pulmonary Critical Care, Shahid Beheshti University of Medical Sciences, Loghman Hakim General Teaching Hospital, Tehran, IranSchool of Persian Medicine, Tehran University of Medical Sciences, Tehran, IranTraditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, IranChronic Respiratory Diseases Research Center, Natoinal Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Persian Medicine Network (PMN), Universal Scientific Education and Research Network (USERN), Tehran, IranDepartment of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranDepartment of Psychiatric Nursing, School of Nursing and Midwifery, Zanjan University of Medical Sciences, Zanjan, IranTraditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran As an inflammatory disease of the lung, asthma is characterized by bronchoconstriction, mucus hypersecretion, inflammatory mediator release, and eosinophil recruitment. Cupping therapy is an ancient method of treatment for a vast range of ailments. This study aimed to evaluate the anti-asthmatic effects of wet cupping therapy (WCT) in a mouse model. A total number of 35 Balb/c mice were randomly divided into five groups (n = 7): Negative and positive control groups were administered Phosphate Buffered Saline (PBS) and ovalbumin (OVA), respectively. The remaining three OVA-challenged groups were treated with budesonide, one session, and two sessions of WCT. Finally, eosinophil counts, the gene expressions, and the protein levels of interleukins IL-5, -13, and -33 were measured in bronchoalveolar lavage fluid (BALF) of mice. Lung tissues were removed and kept for histopathological evaluations. Both eosinophil counts and interleukin levels in BALFs were significantly diminished following WCT. Moreover, WCT prevented hyperplastic growth of goblet cells, overproduction of mucus, and inflammation of peribronchial and perivascular areas of lung tissue of mice compared to positive control group. Interestingly, the anti-inflammatory effects of WCT against asthma were comparable to budesonide. Our data suggested that the anti-asthmatic effects of WCT were mediated by reducing eosinophil trafficking and modulating Th2 inflammatory cytokines, leading to the histological changes of the lung. This may propose WCT as an efficient therapeutic approach to mitigate inflammatory complications of asthma. https://jtim.tums.ac.ir/index.php/jtim/article/view/404Wet cupping therapyAsthmaInflammatory responseInterleukinsPersian medicine |
| spellingShingle | Abbas Joushan Sadegh Rajabi Khosrow Agin Mohammad Hossein Ayati Fatemeh Jafari Babak Daneshfard Sayyed Shamsadin Athari Zeinab Ghahremani Rasool Choopani Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study Traditional and Integrative Medicine Wet cupping therapy Asthma Inflammatory response Interleukins Persian medicine |
| title | Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study |
| title_full | Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study |
| title_fullStr | Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study |
| title_full_unstemmed | Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study |
| title_short | Wet Cupping Therapy Ameliorates the Inflammatory Responses in Mice Model of Allergic Asthma: An Experimental Histopathological Study |
| title_sort | wet cupping therapy ameliorates the inflammatory responses in mice model of allergic asthma an experimental histopathological study |
| topic | Wet cupping therapy Asthma Inflammatory response Interleukins Persian medicine |
| url | https://jtim.tums.ac.ir/index.php/jtim/article/view/404 |
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