Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B
Abstract Chronic hepatitis B is a significant public health problem and complex pathologic process, and unraveling the underlying mechanisms and pathophysiology is of great significance. Data independent acquisition mass spectrometry (DIA-MS) is a label-free quantitative proteomics method that has b...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Proteome Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12953-023-00209-6 |
| _version_ | 1797806829480181760 |
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| author | Bo Wang Qian Zhang Lili Wu Cunliang Deng Meiyan Luo Yu Xie Gang Wu Wen Chen Yunjian Sheng Peng Zhu Gang Qin |
| author_facet | Bo Wang Qian Zhang Lili Wu Cunliang Deng Meiyan Luo Yu Xie Gang Wu Wen Chen Yunjian Sheng Peng Zhu Gang Qin |
| author_sort | Bo Wang |
| collection | DOAJ |
| description | Abstract Chronic hepatitis B is a significant public health problem and complex pathologic process, and unraveling the underlying mechanisms and pathophysiology is of great significance. Data independent acquisition mass spectrometry (DIA-MS) is a label-free quantitative proteomics method that has been successfully applied to the study of a wide range of diseases. The aim of this study was to apply DIA-MS for proteomic analysis of patients with chronic hepatitis B. We performed comprehensive proteomics analysis of protein expression in serum samples from HBV patients and healthy controls by using DIA-MS. Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein network analysis were performed on differentially expressed proteins and were further combined with literature analysis. We successfully identified a total of 3786 serum proteins with a high quantitative performance from serum samples in this study. We identified 310 differentially expressed proteins (DEPs) (fold change > 1.5 and P value < 0.05 as the criteria for a significant difference) between HBV and healthy samples. A total of 242 upregulated proteins and 68 downregulated proteins were among the DEPs. Some protein expression levels were significantly elevated or decreased in patients with chronic hepatitis B, indicating a relation to chronic liver disease, which should be further investigated. |
| first_indexed | 2024-03-13T06:13:18Z |
| format | Article |
| id | doaj.art-25093bfbc64a40458e4c7664bb6f9772 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-03-13T06:13:18Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-25093bfbc64a40458e4c7664bb6f97722023-06-11T11:06:05ZengBMCProteome Science1477-59562023-06-0121111110.1186/s12953-023-00209-6Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis BBo Wang0Qian Zhang1Lili Wu2Cunliang Deng3Meiyan Luo4Yu Xie5Gang Wu6Wen Chen7Yunjian Sheng8Peng Zhu9Gang Qin10Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityDepartment of Gastroenterology, Suining First Pepole’s HospitalDepartment of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityCollege of Graduate, Southwest Medical UniversityCollege of Graduate, Southwest Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityDepartment of Infectious Diseases, The Affiliated Hospital of Southwest Medical UniversityDepartment of Gastroenterology, Suining First Pepole’s HospitalDepartment of Gastroenterology, Suining First Pepole’s HospitalAbstract Chronic hepatitis B is a significant public health problem and complex pathologic process, and unraveling the underlying mechanisms and pathophysiology is of great significance. Data independent acquisition mass spectrometry (DIA-MS) is a label-free quantitative proteomics method that has been successfully applied to the study of a wide range of diseases. The aim of this study was to apply DIA-MS for proteomic analysis of patients with chronic hepatitis B. We performed comprehensive proteomics analysis of protein expression in serum samples from HBV patients and healthy controls by using DIA-MS. Gene Ontology (GO) terms, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein network analysis were performed on differentially expressed proteins and were further combined with literature analysis. We successfully identified a total of 3786 serum proteins with a high quantitative performance from serum samples in this study. We identified 310 differentially expressed proteins (DEPs) (fold change > 1.5 and P value < 0.05 as the criteria for a significant difference) between HBV and healthy samples. A total of 242 upregulated proteins and 68 downregulated proteins were among the DEPs. Some protein expression levels were significantly elevated or decreased in patients with chronic hepatitis B, indicating a relation to chronic liver disease, which should be further investigated.https://doi.org/10.1186/s12953-023-00209-6Chronic hepatitis BHepatitis B virusCHBDIA-MS |
| spellingShingle | Bo Wang Qian Zhang Lili Wu Cunliang Deng Meiyan Luo Yu Xie Gang Wu Wen Chen Yunjian Sheng Peng Zhu Gang Qin Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B Proteome Science Chronic hepatitis B Hepatitis B virus CHB DIA-MS |
| title | Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B |
| title_full | Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B |
| title_fullStr | Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B |
| title_full_unstemmed | Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B |
| title_short | Data-independent acquisition-based mass spectrometry(DIA-MS) for quantitative analysis of patients with chronic hepatitis B |
| title_sort | data independent acquisition based mass spectrometry dia ms for quantitative analysis of patients with chronic hepatitis b |
| topic | Chronic hepatitis B Hepatitis B virus CHB DIA-MS |
| url | https://doi.org/10.1186/s12953-023-00209-6 |
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