Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China
Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of...
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MDPI AG
2011-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/12/9/5698/ |
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| author | Kaiyun Guan Junbo Yang Zhonglang Wang Weibang Sun Le Zhang |
| author_facet | Kaiyun Guan Junbo Yang Zhonglang Wang Weibang Sun Le Zhang |
| author_sort | Kaiyun Guan |
| collection | DOAJ |
| description | Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species. |
| first_indexed | 2024-04-11T23:26:19Z |
| format | Article |
| id | doaj.art-250ca93ed3de4a44a21d2c9dd703b598 |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-11T23:26:19Z |
| publishDate | 2011-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-250ca93ed3de4a44a21d2c9dd703b5982022-12-22T03:57:17ZengMDPI AGInternational Journal of Molecular Sciences1422-00672011-09-011295698570410.3390/ijms12095698Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest ChinaKaiyun GuanJunbo YangZhonglang WangWeibang SunLe ZhangHibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.http://www.mdpi.com/1422-0067/12/9/5698/Hibiscus aridicolaendangered plantSSR markerspopulation structurepopulation genetics |
| spellingShingle | Kaiyun Guan Junbo Yang Zhonglang Wang Weibang Sun Le Zhang Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China International Journal of Molecular Sciences Hibiscus aridicola endangered plant SSR markers population structure population genetics |
| title | Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China |
| title_full | Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China |
| title_fullStr | Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China |
| title_full_unstemmed | Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China |
| title_short | Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China |
| title_sort | isolation and characterization of microsatellite loci for hibiscus aridicola malvaceae an endangered plant endemic to the dry hot valleys of jinsha river in southwest china |
| topic | Hibiscus aridicola endangered plant SSR markers population structure population genetics |
| url | http://www.mdpi.com/1422-0067/12/9/5698/ |
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