A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission.
Mosquito saliva is a vehicle for the transmission of vector borne pathogens such as Plasmodium parasites and different arboviruses. Despite the key role of the salivary glands in the process of disease transmission, knowledge of host-pathogen interactions taking place within this organ is very limit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-10-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010881 |
| _version_ | 1811329317766955008 |
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| author | Dennis Klug Katharina Arnold Raquel Mela-Lopez Eric Marois Stéphanie A Blandin |
| author_facet | Dennis Klug Katharina Arnold Raquel Mela-Lopez Eric Marois Stéphanie A Blandin |
| author_sort | Dennis Klug |
| collection | DOAJ |
| description | Mosquito saliva is a vehicle for the transmission of vector borne pathogens such as Plasmodium parasites and different arboviruses. Despite the key role of the salivary glands in the process of disease transmission, knowledge of host-pathogen interactions taking place within this organ is very limited. To improve the experimental tractability of the salivary glands, we have generated fluorescent reporter lines in the African malaria mosquito Anopheles coluzzii using the salivary gland-specific promoters of the anopheline antiplatelet protein (AAPP), the triple functional domain protein (TRIO) and saglin (SAG) coding genes. Promoter activity was specifically observed in the distal-lateral lobes or in the median lobe of the salivary glands. Besides a comparison of the expression patterns of the selected promoters, the fluorescent probes allowed us to evaluate the inducibility of the selected promoters upon blood feeding and to measure intracellular redox changes. We also combined the aapp-DsRed fluorescent reporter line with a pigmentation-deficient yellow(-) mosquito mutant to assess the feasibility of in vivo microscopy of parasitized salivary glands. This combination allowed locating the salivary gland through the cuticle and imaging of individual sporozoites in vivo, which facilitates live imaging studies of salivary gland colonization by Plasmodium sporozoites. |
| first_indexed | 2024-04-13T15:41:46Z |
| format | Article |
| id | doaj.art-250ef30f9ae3414391959fa75d4c8637 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-13T15:41:46Z |
| publishDate | 2022-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-250ef30f9ae3414391959fa75d4c86372022-12-22T02:41:08ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-10-011810e101088110.1371/journal.ppat.1010881A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission.Dennis KlugKatharina ArnoldRaquel Mela-LopezEric MaroisStéphanie A BlandinMosquito saliva is a vehicle for the transmission of vector borne pathogens such as Plasmodium parasites and different arboviruses. Despite the key role of the salivary glands in the process of disease transmission, knowledge of host-pathogen interactions taking place within this organ is very limited. To improve the experimental tractability of the salivary glands, we have generated fluorescent reporter lines in the African malaria mosquito Anopheles coluzzii using the salivary gland-specific promoters of the anopheline antiplatelet protein (AAPP), the triple functional domain protein (TRIO) and saglin (SAG) coding genes. Promoter activity was specifically observed in the distal-lateral lobes or in the median lobe of the salivary glands. Besides a comparison of the expression patterns of the selected promoters, the fluorescent probes allowed us to evaluate the inducibility of the selected promoters upon blood feeding and to measure intracellular redox changes. We also combined the aapp-DsRed fluorescent reporter line with a pigmentation-deficient yellow(-) mosquito mutant to assess the feasibility of in vivo microscopy of parasitized salivary glands. This combination allowed locating the salivary gland through the cuticle and imaging of individual sporozoites in vivo, which facilitates live imaging studies of salivary gland colonization by Plasmodium sporozoites.https://doi.org/10.1371/journal.ppat.1010881 |
| spellingShingle | Dennis Klug Katharina Arnold Raquel Mela-Lopez Eric Marois Stéphanie A Blandin A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. PLoS Pathogens |
| title | A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. |
| title_full | A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. |
| title_fullStr | A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. |
| title_full_unstemmed | A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. |
| title_short | A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission. |
| title_sort | toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission |
| url | https://doi.org/10.1371/journal.ppat.1010881 |
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