The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model
Background: It has been shown that the Juglans regia leaves have hypoglycemic, diuretic and blood pressure reduction properties. The objective of the present study was to examine the histopathology effects of Juglans regia leaves on diabetes mellitus in rats. Material and Methods: Forty-eight Wistar...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2012-12-01
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| Series: | Iranian South Medical Journal |
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| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-292&slc_lang=en&sid=1 |
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| author | Jamshid Mohammadi Ali Mirzaie Arsalan Azizi Amrollah Roozbehi Hamdollah Delaviz |
| author_facet | Jamshid Mohammadi Ali Mirzaie Arsalan Azizi Amrollah Roozbehi Hamdollah Delaviz |
| author_sort | Jamshid Mohammadi |
| collection | DOAJ |
| description | Background: It has been shown that the Juglans regia leaves have hypoglycemic, diuretic and blood pressure reduction properties. The objective of the present study was to examine the histopathology effects of Juglans regia leaves on diabetes mellitus in rats. Material and Methods: Forty-eight Wistar rats (150- 200 g) were randomized into six groups of 8 animals. Groups III, IV, V and VI received intraperitoneal injection of streptozotocin to make diabetic model. Blood glucose was measured by glucometer after 72 hours. After 10 days, Group II, IV and V received 400, 200 and 400 mg/kg Juglans regia extract, respectively and group VI treated with 4 mg/kg glybenclamid for four weeks. Group I were fed with normal diet and group III received distilled water (diabetic control). The body weight and blood glucose were measured in every week. The number of β cells and diameter of islets of Langerhans were determined using hematoxylin-floxin staining. The collected data was analyzed by the SPSS software using one-way ANOVA. Results: The number of β cells and diameter of islets decreased significantly in diabetic control compare to the normal group. Treatment with 400 mg/kg of Juglans regia extract showed a significant decrease in blood glucose, a significant increase in diameter of islets and number of β cells compared to diabetic control group. The pancreas / body weight ratio increased in diabetic rats compare to the treatment group. Conclusion: The administration of Juglans regia extract can cause recovery of β cells number and improve the size of islets of Langerhans in diabetic model rats. |
| first_indexed | 2024-12-14T05:22:58Z |
| format | Article |
| id | doaj.art-250fcf0baa6f40a6aac10308d0981225 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-14T05:22:58Z |
| publishDate | 2012-12-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-250fcf0baa6f40a6aac10308d09812252022-12-21T23:15:36ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542012-12-01154293302The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats modelJamshid Mohammadi0Ali Mirzaie1Arsalan Azizi2Amrollah Roozbehi3Hamdollah Delaviz4 Herbal Medicine Research Center, Yasouj University of Medical Sceinecs, Yasouj, IRAN Department of Biochemistry, Herbal Medicine Research Center, Yasouj University of Medical Sceinecs, Yasouj, IRAN Department of Pathology, School of Medicine, Yasouj University of Medical Sceinecs, Yasouj, IRAN Department of Anatomy, School of Medicine, Yasouj University of Medical Sceinecs, Yasouj, IRAN Department of Anatomy, School of Medicine, Yasouj University of Medical Sceinecs, Yasouj, IRAN Background: It has been shown that the Juglans regia leaves have hypoglycemic, diuretic and blood pressure reduction properties. The objective of the present study was to examine the histopathology effects of Juglans regia leaves on diabetes mellitus in rats. Material and Methods: Forty-eight Wistar rats (150- 200 g) were randomized into six groups of 8 animals. Groups III, IV, V and VI received intraperitoneal injection of streptozotocin to make diabetic model. Blood glucose was measured by glucometer after 72 hours. After 10 days, Group II, IV and V received 400, 200 and 400 mg/kg Juglans regia extract, respectively and group VI treated with 4 mg/kg glybenclamid for four weeks. Group I were fed with normal diet and group III received distilled water (diabetic control). The body weight and blood glucose were measured in every week. The number of β cells and diameter of islets of Langerhans were determined using hematoxylin-floxin staining. The collected data was analyzed by the SPSS software using one-way ANOVA. Results: The number of β cells and diameter of islets decreased significantly in diabetic control compare to the normal group. Treatment with 400 mg/kg of Juglans regia extract showed a significant decrease in blood glucose, a significant increase in diameter of islets and number of β cells compared to diabetic control group. The pancreas / body weight ratio increased in diabetic rats compare to the treatment group. Conclusion: The administration of Juglans regia extract can cause recovery of β cells number and improve the size of islets of Langerhans in diabetic model rats.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-292&slc_lang=en&sid=1Juglans regia leaf diabetes mellitus β cells rat islets of Langerhans |
| spellingShingle | Jamshid Mohammadi Ali Mirzaie Arsalan Azizi Amrollah Roozbehi Hamdollah Delaviz The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model Iranian South Medical Journal Juglans regia leaf diabetes mellitus β cells rat islets of Langerhans |
| title | The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model |
| title_full | The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model |
| title_fullStr | The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model |
| title_full_unstemmed | The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model |
| title_short | The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model |
| title_sort | effects of hydroalcoholic extract of juglans regia leaf on histological changes of langerhans islet in diabetic rats model |
| topic | Juglans regia leaf diabetes mellitus β cells rat islets of Langerhans |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-292&slc_lang=en&sid=1 |
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