Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells
Background/Aims: Colon cancer, also known as colorectal cancer (CRC), is one of the most common malignant tumors globally. Although significant advances have been made for developing novel therapeutics, the mechanisms of progression of colorectal cancer are still poorly understood. Methods: In this...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2018-08-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/492992 |
| _version_ | 1811337258386587648 |
|---|---|
| author | Kaiming Wu Jun Ma Yanfeng Zhan Kuanzhi Liu Ziyin Ye Jianhui Chen Kaiwu Xu Hongli Huang Yulong He |
| author_facet | Kaiming Wu Jun Ma Yanfeng Zhan Kuanzhi Liu Ziyin Ye Jianhui Chen Kaiwu Xu Hongli Huang Yulong He |
| author_sort | Kaiming Wu |
| collection | DOAJ |
| description | Background/Aims: Colon cancer, also known as colorectal cancer (CRC), is one of the most common malignant tumors globally. Although significant advances have been made for developing novel therapeutics, the mechanisms of progression of colorectal cancer are still poorly understood. Methods: In this study, we identified down-regulation of microRNA-214 (miR-214) as the contributing factor for CRC. Mitochondrial transcription factor A (TFAM) and miR-214 expression in tumor samples from colorectal cancer patients and cancer cell lines were examined by reverse transcription and real-Time PCR (qPCR) or Western Blotting. Results: Our data demonstrated that miR-214 was significantly down-regulated in the tissue samples from CRC patients as well as CRC derived cell lines. TFAM overexpression was also observed in CRC patients and identified as a target for miR-214. Knockdown of TFAM by miR-214 mimics significantly inhibited the proliferation of CRC cell lines. Also, down-regulation of TFAM inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nuclear translocation and the expression of NF-κB depended genes. Conclusion: In conclusion, our data suggested that down-regulation of MiR-214 contributed to the enhanced TFAM expression and decreased proliferation of CRC cells. |
| first_indexed | 2024-04-13T17:52:12Z |
| format | Article |
| id | doaj.art-25103134806949a480c3bde36c481db6 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-13T17:52:12Z |
| publishDate | 2018-08-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-25103134806949a480c3bde36c481db62022-12-22T02:36:38ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-08-0149254555410.1159/000492992492992Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer CellsKaiming WuJun MaYanfeng ZhanKuanzhi LiuZiyin YeJianhui ChenKaiwu XuHongli HuangYulong HeBackground/Aims: Colon cancer, also known as colorectal cancer (CRC), is one of the most common malignant tumors globally. Although significant advances have been made for developing novel therapeutics, the mechanisms of progression of colorectal cancer are still poorly understood. Methods: In this study, we identified down-regulation of microRNA-214 (miR-214) as the contributing factor for CRC. Mitochondrial transcription factor A (TFAM) and miR-214 expression in tumor samples from colorectal cancer patients and cancer cell lines were examined by reverse transcription and real-Time PCR (qPCR) or Western Blotting. Results: Our data demonstrated that miR-214 was significantly down-regulated in the tissue samples from CRC patients as well as CRC derived cell lines. TFAM overexpression was also observed in CRC patients and identified as a target for miR-214. Knockdown of TFAM by miR-214 mimics significantly inhibited the proliferation of CRC cell lines. Also, down-regulation of TFAM inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nuclear translocation and the expression of NF-κB depended genes. Conclusion: In conclusion, our data suggested that down-regulation of MiR-214 contributed to the enhanced TFAM expression and decreased proliferation of CRC cells.https://www.karger.com/Article/FullText/492992Colorectal cancerTFAMmiR-214ProliferationNF-κB |
| spellingShingle | Kaiming Wu Jun Ma Yanfeng Zhan Kuanzhi Liu Ziyin Ye Jianhui Chen Kaiwu Xu Hongli Huang Yulong He Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells Cellular Physiology and Biochemistry Colorectal cancer TFAM miR-214 Proliferation NF-κB |
| title | Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells |
| title_full | Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells |
| title_fullStr | Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells |
| title_full_unstemmed | Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells |
| title_short | Down-Regulation of MicroRNA-214 Contributed to the Enhanced Mitochondrial Transcription Factor A and Inhibited Proliferation of Colorectal Cancer Cells |
| title_sort | down regulation of microrna 214 contributed to the enhanced mitochondrial transcription factor a and inhibited proliferation of colorectal cancer cells |
| topic | Colorectal cancer TFAM miR-214 Proliferation NF-κB |
| url | https://www.karger.com/Article/FullText/492992 |
| work_keys_str_mv | AT kaimingwu downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT junma downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT yanfengzhan downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT kuanzhiliu downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT ziyinye downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT jianhuichen downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT kaiwuxu downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT honglihuang downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells AT yulonghe downregulationofmicrorna214contributedtotheenhancedmitochondrialtranscriptionfactoraandinhibitedproliferationofcolorectalcancercells |