DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture
We examined angiogenesis-related gene expression profiles using collagen gel culture and a DNA chip. After isolation of total RNA from cultures before and after capillary tube formation, a mouse whole-genome array study was performed. Seventy-three out of over 35,000 transcripts were expressed after...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2008-01-01
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| Series: | Clinical Medicine Insights: Cardiology |
| Online Access: | https://doi.org/10.4137/CMC.S532 |
| _version_ | 1818455988370407424 |
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| author | Masumi Akita Keiko Fujita |
| author_facet | Masumi Akita Keiko Fujita |
| author_sort | Masumi Akita |
| collection | DOAJ |
| description | We examined angiogenesis-related gene expression profiles using collagen gel culture and a DNA chip. After isolation of total RNA from cultures before and after capillary tube formation, a mouse whole-genome array study was performed. Seventy-three out of over 35,000 transcripts were expressed after capillary tube formation. The majority of genes did not show any significant differences between before and after capillary tube formation. However, there were 7 up-regulated genes; tumor necrosis factor alpha-induced protein (Tnfaip) 2, vascular endothelial growth factor b (Vegfb), corticotropin releasing hormone receptor (Crhr) 2, vascular endothelial growth factor C (Vegfc), fibroblast growth factor (Fgf) 6, protein O-fucosyltransferase (Pofut) 1, hypoxia inducible factor 1 alpha subunit (Hif-1a). Eleven down-regulated genes were identified; ras homolog gene family member B (RhoB), Fgf-2, Thrombospondin (Thbs) 1, Angiogenin (Ang) 3, guanine nucleotide binding protein alpha (Gna) 13, tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a), plasminogen (Plg), Elk3, upstream binding protein (Ubp) 1, integrin alpha V (Itgav), mitogen activated protein kinase (Mapk) 14. These up- and down-regulated genes identified in this study offer the strategies which improve the myocardial substrate during and following a myocardial infarction, such as the regrowth of functional blood vessels to the ischemic myocardium. |
| first_indexed | 2024-12-14T22:19:31Z |
| format | Article |
| id | doaj.art-25118c4b437b49eeb0a0fd89ef54787e |
| institution | Directory Open Access Journal |
| issn | 1179-5468 |
| language | English |
| last_indexed | 2024-12-14T22:19:31Z |
| publishDate | 2008-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Clinical Medicine Insights: Cardiology |
| spelling | doaj.art-25118c4b437b49eeb0a0fd89ef54787e2022-12-21T22:45:32ZengSAGE PublishingClinical Medicine Insights: Cardiology1179-54682008-01-01210.4137/CMC.S532DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel CultureMasumi Akita0Keiko Fujita1Division of Morphological Science, Biomedical Research Center, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, Japan.Department of Anatomy, Saitama Medical University, 38 Moroyama, Iruma-gun, Saitama 350-0495, Japan.We examined angiogenesis-related gene expression profiles using collagen gel culture and a DNA chip. After isolation of total RNA from cultures before and after capillary tube formation, a mouse whole-genome array study was performed. Seventy-three out of over 35,000 transcripts were expressed after capillary tube formation. The majority of genes did not show any significant differences between before and after capillary tube formation. However, there were 7 up-regulated genes; tumor necrosis factor alpha-induced protein (Tnfaip) 2, vascular endothelial growth factor b (Vegfb), corticotropin releasing hormone receptor (Crhr) 2, vascular endothelial growth factor C (Vegfc), fibroblast growth factor (Fgf) 6, protein O-fucosyltransferase (Pofut) 1, hypoxia inducible factor 1 alpha subunit (Hif-1a). Eleven down-regulated genes were identified; ras homolog gene family member B (RhoB), Fgf-2, Thrombospondin (Thbs) 1, Angiogenin (Ang) 3, guanine nucleotide binding protein alpha (Gna) 13, tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a), plasminogen (Plg), Elk3, upstream binding protein (Ubp) 1, integrin alpha V (Itgav), mitogen activated protein kinase (Mapk) 14. These up- and down-regulated genes identified in this study offer the strategies which improve the myocardial substrate during and following a myocardial infarction, such as the regrowth of functional blood vessels to the ischemic myocardium.https://doi.org/10.4137/CMC.S532 |
| spellingShingle | Masumi Akita Keiko Fujita DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture Clinical Medicine Insights: Cardiology |
| title | DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture |
| title_full | DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture |
| title_fullStr | DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture |
| title_full_unstemmed | DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture |
| title_short | DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture |
| title_sort | dna micro array gene expression profiling of angiogenesis in collagen gel culture |
| url | https://doi.org/10.4137/CMC.S532 |
| work_keys_str_mv | AT masumiakita dnamicroarraygeneexpressionprofilingofangiogenesisincollagengelculture AT keikofujita dnamicroarraygeneexpressionprofilingofangiogenesisincollagengelculture |